User talk:Spitzer Gudrun: Difference between revisions
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(Created page with "= Asc gutest 1= == Application in HRR == :::: '''As: Ascorbate sodium salt''' (Sodium L-ascorbate); Sigma A 7631, 100 g, store at RT; FW = 198.1. :::: <span style="colo...") |
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:::»'''O2k manual titrations:''' [[MiPNet09.12]] | :::»'''O2k manual titrations:''' [[MiPNet09.12]] | ||
::::* Titration volume ('''2-mL O2k-chamber'''): 5 µL 800mM using a 25 µL Hamilton syringe. | ::::* Titration volume ('''2-mL O2k-chamber'''): 5 µL of 800mM stock solution using a 25 µL Hamilton syringe. | ||
::::* Titration volume ('''0.5-mL O2k-chamber'''): 1 µL 1000mM using a 10 µL Hamilton syringe | ::::* Titration volume ('''0.5-mL O2k-chamber'''): 1 µL of 1000mM stock solution using a 10 µL Hamilton syringe | ||
::::* Final concentration: 2 mM. | ::::* Final concentration: 2 mM. | ||
= Asc gutest 2 = | = Asc gutest 2 = | ||
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:::»'''O2k manual titrations:''' [[MiPNet09.12]] | :::»'''O2k manual titrations:''' [[MiPNet09.12]] | ||
::::* Titration volume ('''2-mL O2k-chamber'''): 5 µL 800 mM using a 25 µL Hamilton syringe. | ::::* Titration volume ('''2-mL O2k-chamber'''): 5 µL of 800 mM stock solution using a 25 µL Hamilton syringe. | ||
::::* Final concentration: 2 mM. | ::::* Final concentration: 2 mM. | ||
| Line 58: | Line 58: | ||
:::»'''O2k manual titrations:''' [[MiPNet09.12]] | :::»'''O2k manual titrations:''' [[MiPNet09.12]] | ||
::::* Titration volume ('''0.5-mL O2k-chamber'''): 1 µL 1000 mM using a 10 µL Hamilton syringe. | ::::* Titration volume ('''0.5-mL O2k-chamber'''): 1 µL of 1000 mM stock solution using a 10 µL Hamilton syringe. | ||
::::* Final concentration: 2 mM. | ::::* Final concentration: 2 mM. | ||
Revision as of 08:54, 22 October 2020
Asc gutest 1
Application in HRR
- As: Ascorbate sodium salt (Sodium L-ascorbate); Sigma A 7631, 100 g, store at RT; FW = 198.1.
- Caution: Light sensitive (store solution in a dark glass vial).
'
- Preparation of 800 mM stock solution (dissolved in H2O) for use in 2-mL O2k-chamber:
- Weigh 1.584 g of ascorbate sodium salt and dissolve in 10 mL H2O
- To prevent autoxidation, adjust pH to approx. 6 with ascorbic acid (a 137.6 mg.mL-1 solution of pH 2).
- Divide into 0.2 mL portions.
- Store frozen at -20 °C.
- Preparation of 1000 mM stock solution (dissolved in H2O) for use in 0.5-mL O2k-chamber:
- Weigh 1.980 g of ascorbate sodium salt and dissolve in 10 mL H2O
- To prevent autoxidation, adjust pH to approx. 6 with ascorbic acid (a 137.6 mg.mL-1 solution of pH 2).
- Divide into 0.2 mL portions.
- Store frozen at -20 °C.
- »O2k manual titrations: MiPNet09.12
- Titration volume (2-mL O2k-chamber): 5 µL of 800mM stock solution using a 25 µL Hamilton syringe.
- Titration volume (0.5-mL O2k-chamber): 1 µL of 1000mM stock solution using a 10 µL Hamilton syringe
- Final concentration: 2 mM.
Asc gutest 2
Application in HRR
- As: Ascorbate sodium salt (Sodium L-ascorbate); Sigma A 7631, 100 g, store at RT; FW = 198.1.
- Caution: Light sensitive (store solution in a dark glass vial).
- Preparation of 800 mM stock solution (dissolved in H2O) for 2-mL O2k-chamber:
- Weigh 1.584 g of ascorbate sodium salt and dissolve in 10 mL H2O
- To prevent autoxidation, adjust pH to approx. 6 with ascorbic acid (a 137.6 mg.mL-1 solution of pH 2).
- Divide into 0.2 mL portions.
- Store frozen at -20 °C.
- »O2k manual titrations: MiPNet09.12
- Titration volume (2-mL O2k-chamber): 5 µL of 800 mM stock solution using a 25 µL Hamilton syringe.
- Final concentration: 2 mM.
- Preparation of 1000 mM stock solution (dissolved in H2O) for 0.5-mL O2k-chamber:
- Weigh 1.980 g of ascorbate sodium salt and dissolve in 10 mL H2O
- To prevent autoxidation, adjust pH to approx. 6 with ascorbic acid (a 137.6 mg.mL-1 solution of pH 2).
- Divide into 0.2 mL portions.
- Store frozen at -20 °C.
- »O2k manual titrations: MiPNet09.12
- Titration volume (0.5-mL O2k-chamber): 1 µL of 1000 mM stock solution using a 10 µL Hamilton syringe.
- Final concentration: 2 mM.
