Difference between revisions of "Template:SUIT text D061"

Revision as of 15:16, 19 July 2019

SUIT-003 O2 ce D061 protocol is a control for the SUIT-003 O2 ce D060, with the addition of the carrier DMSO instead of the plasma membrane-permeable succinate (MitoKit-CII/Succinate-nv) and plasma membrane-permeable malonate (MitoKit-CII/Malonate-nv). Moreover, the SUIT-003 O2 ce D061 protocol allows evaluating the coupling control of living cells. Respiratory capacities are tested in a sequence of coupling states: ROUTINE and ET. Optionally, to study LEAK-respiration, the phosphorylation system might be inhibited by oligomycin. The final concentration of oligomycin has to be carefully optimized for various cell types, to minimize the inhibitory effect on the electron transfer system which would lead to an underestimation of ET-capacity.

Revision as of 15:13, 19 July 2019 (view source) Antunes Diana (talk \| contribs) (Created page with "SUIT-003 O2 ce D061 protocol is a control for the SUIT-003 O2 ce D060, with the addition of the carrier DMSO instead of the plasma membrane-...")		Revision as of 15:16, 19 July 2019 (view source) Iglesias-Gonzalez Javier (talk \| contribs) Newer edit →
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	SUIT-003 O2 ce D061 protocol is a control for the [[SUIT-003 O2 ce D060]], with the addition of the [[Carrier control titrations\|carrier]] DMSO instead of the plasma membrane-permeable succinate ([[[MitoKit-CII/Succinate-nv]]) and plasma membrane-permeable malonate ([[MitoKit-CII/Malonate-nv]]). Moreover, the SUIT-003 O2 ce D061 protocol allows evaluating the [[coupling control state \|coupling control]] of living cells. Respiratory capacities are tested in a sequence of coupling states: [[ROUTINE]] and [[ET]]. Optionally, to study [[LEAK-respiration]], the [[phosphorylation system]] might be inhibited by [[oligomycin]]. The final concentration of [[oligomycin]] has to be carefully optimized for various cell types, to minimize the inhibitory effect on the [[Electron transfer-pathway\|electron transfer system]] which would lead to an underestimation of [[ET-capacity]].		SUIT-003 O2 ce D061 protocol is a control for the [[SUIT-003 O2 ce D060]], with the addition of the [[Carrier control titrations\|carrier]] DMSO instead of the plasma membrane-permeable succinate ([[MitoKit-CII/Succinate-nv]]) and plasma membrane-permeable malonate ([[MitoKit-CII/Malonate-nv]]). Moreover, the SUIT-003 O2 ce D061 protocol allows evaluating the [[coupling control state \|coupling control]] of living cells. Respiratory capacities are tested in a sequence of coupling states: [[ROUTINE]] and [[ET]]. Optionally, to study [[LEAK-respiration]], the [[phosphorylation system]] might be inhibited by [[oligomycin]]. The final concentration of [[oligomycin]] has to be carefully optimized for various cell types, to minimize the inhibitory effect on the [[Electron transfer-pathway\|electron transfer system]] which would lead to an underestimation of [[ET-capacity]].