Difference between revisions of "Template:Current week ecosystem agenda"

Latest revision as of 16:15, 6 May 2024

Oroboros declares solidarity against the Russian aggression-war in Ukraine and against all aggression-wars worldwide. - »More details

-» A future for Ukraine is the future of its science

2024 Week 19

May-06 Mo

What is the effective concentration of digitonin for complete plasma membrane permeabilization?

It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane.
- »Digitonin«, »SUIT-010«
- communicated by »Schmitt Sabine« and »Timon-Gomez Alba«

May-07 Tu

Tributes to pioneers in bioenergetics will provide a fresh look at concepts in a state of flow rather than as fixed paradigms, which tend to be forgotten or distorted without wandering in the garden of memories.

- »Tributes to pioneers in bioenergetics - wandering in the garden of memories« - a new BEC Publication Series

May-08 We

May-09 Th

"The methodology exploited in this work opens the possibility to extrapolate the photosynthetic functionality of microalgae in dense cultures typical of industrial cultivation plants already at the lab-scale"

Vera-Vives AM, Michelberger T, Morosinotto T, Perin G (2024) Assessment of photosynthetic activity in dense microalgae cultures using oxygen production. Plant Physiol Biochem »Bioblast link«

from the O2k-Network »IT Padova Morosinotto T«

May-10 Fr

Next Week

2024 Week 20

May-13 Mo

Do you see fluctuations of O₂ slope negative following a pattern similar to the Peltier power, but without block temperature variation?

Placing the O2k under direct airflow of an air conditioning equipment may lead to disturbances in the O₂ slope negative.
- »Fluctuations of O₂ slope negative«
- communicated by »Cardoso Luiza« and »Grings Mateus«

May-14 Tu

May-15 We

May-16 Th

"The sample holder protects (...) fragile samples from potential damage caused by stirring the medium."

- »MiPNet27.07 Sample Holder«
- communicated by »Willis Jaime R« and »Timon-Gomez Alba«

May-17 Fr

@@ Line 1: / Line 1: @@
 {{Template:Science for Ukraine}}
-Week 11
-* '''Mar-11 Mo'''
+Week 19
+* '''May-06 Mo'''
 :{|
 | width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
-|''' Why is it important to perform respirometry with permeabilized muscle fibers (pfi) under a high [O<sub>2</sub>] regime? '''<br>
+|''' What is the effective concentration of digitonin for complete plasma membrane permeabilization?'''<br>
-Long diffusion distances in the pfi result in an O<sub>2</sub> diffusion limitation, which is overcome by increasing the O<sub>2</sub> partial pressure inside the chamber. Proper instrumental background correction is a must and O<sub>2</sub> dependence of ROS production should be considered.   <br>
+It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane. <br>
-- »'''[[Permeabilized muscle fibers]]'''«, »'''[[Oxygen flux - instrumental background]]'''« <br>
+- »'''[[Digitonin]]'''«, »'''[[SUIT-010]]'''« <br>
-- communicated by »[[Grings Mateus]]« and »[[Cardoso Luiza]]«
+- communicated by »[[Schmitt Sabine]]« and »[[Timon-Gomez Alba]]«
 |}
 ----
-* '''Mar-12 Tu'''
+* '''May-07 Tu'''
-----
-* '''Mar-13 We'''
 :{|
-| width="100" align="center" |[[Image:BEC-logo.png|100px|link=https://www.bioenergetics-communications.org/index.php/bec |Bioenergetics Communications]]
+| width="110" align="center" | [[File:Tributes to pioneers icon.jpg|left|80px|BEC Tributes to pioneers in bioenergetics|link=Tributes to pioneers in bioenergetics]]
-|'''''Rewarded'' Open Access - compensating authors'''<br>
+|'''Tributes to pioneers in bioenergetics''' will provide a fresh look at concepts in a state of flow rather than as fixed paradigms, which tend to be forgotten or distorted without wandering in the garden of memories.<br>
-Together with the MiP''society'', BEC is raising funds to reward the first 10 papers accepted for publication in BEC in 2024: 600 EUR to attend EBEC2024 and 2-year MiP''membership''.
+- »'''[[Tributes to pioneers in bioenergetics |Tributes to pioneers in bioenergetics - wandering in the garden of memories]]'''« - a new BEC Publication Series<br>
--»[https://whydonate.com/en/fundraising/BEC Support open access in science]«<br>
-- »[https://www.bioenergetics-communications.org/index.php/bec/about/submissions Submit a manuscript]«
 |}
 ----
-* '''Mar-14 Th'''
+* '''May-08 We'''
+----
+* '''May-09 Th'''
 :{|
 | width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|'''Mitochondrial ultrastructure and respiration are affected during EDL [''Extensor digitorum longus'' muscle] aging.''' <br>
+|'''"The methodology exploited in this work opens the possibility to extrapolate the photosynthetic functionality of microalgae in dense cultures typical of industrial cultivation plants already at the lab-scale"''' <br>
-* Fernando R, Shindyapina AV, Ost M, Santesmasses D, Hu Y, Tyshkovskiy A, Yim SH, Weiss J, Gladyshev VN, Grune T, Castro JP (2023) Downregulation of mitochondrial metabolism is a driver for fast skeletal muscle loss during mouse aging.  [[Fernando 2023 Commun Biol|»Bioblast link«]]
+* Vera-Vives AM, Michelberger T, Morosinotto T, Perin G (2024) Assessment of photosynthetic activity in dense microalgae cultures using oxygen production. Plant Physiol Biochem [[Vera-Vives 2024 Plant Physiol Biochem|»Bioblast link«]]
-:from the O2k-Network »[[DE Leipzip Ost M]]«
+:from the O2k-Network »[[IT Padova Morosinotto T]]«
 |}
 ----
-* '''Mar-15 Fr'''
+* '''May-10 Fr'''
 ----
+'''<big>Next Week</big>'''
-'''<big>Next Week</big>'''
+Week 20
-Week 12
-* '''Mar-18 Mo'''
+* '''May-13 Mo'''
-::::: '''[[EU-METAHEART MC2 Meeting 2024 Antalya TR]]''' Mar 18-21
-----
-* '''Mar-19 Tu'''
 :{|
-| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Info: take care]]
-|''' What if ''Alert: Error in titrations!'' appears in your O2 analysis template file?  '''<br>
+|'''Do you see fluctuations of O<sub>2</sub> slope negative following a pattern similar to the Peltier power, but without block temperature variation?'''<br>
-The O2 analysis template recognizes discrepancies between the protocol selected in the first tab and the imported marks. Check the titration sequence and mark names against the protocol copied from the DLD file.<br>
+Placing the O2k under direct airflow of an air conditioning equipment may lead to disturbances in the O<sub>2</sub> slope negative.
-- »'''[[MiPNet24.06 Oxygen flux analysis - DatLab 7.4|O<sub>2</sub> flux analysis]]'''«<br>
+<br>
-- communicated by »[[Timon-Gomez Alba]]« and »[[Leo Elettra]]«
+- »'''[[Oxygen_flux_-_instrumental_background#Fluctuations_of_O2_slope_negative|Fluctuations of O<sub>2</sub> slope negative]]'''«<br>
+- communicated by »[[Cardoso Luiza]]« and »[[Grings Mateus]]«
 |}
 ----
-* '''Mar-20 We'''
+* '''May-14 Tu'''
+----
+* '''May-15 We'''
 ----
-* '''Mar-21 Th'''
+* '''May-16 Th'''
 :{|
 | width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|''' Do you handle well your Hamilton syringes? What is a cleaning cycle?'''<br>
+|''' "The sample holder protects (...) fragile samples from potential damage caused by stirring the medium."'''<br>
-"A syringe cleaning cycle consists of filling an empty syringe with a washing solution while immersing [...] also the base of the barrel into the washing solution, without fully removing the plunger, and emptying the syringe into the waste beaker". <br>
+- »'''[[MiPNet27.07 Sample Holder]]'''«<br>
-- »'''[[MiPNet19.14 SOP Hamilton microsyringes]]'''«<br>
+- communicated by »[[Willis Jaime R]]« and »[[Timon-Gomez Alba]]«
-- communicated by »[[Baglivo Eleonora]]« and »[[Timon-Gomez Alba]]«
 |}
 ----
-* '''Mar-22 Fr'''
+* '''May-17 Fr'''
 ----