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Difference between revisions of "Talk:WG1 MitoEAGLE protocols, terminology, documentation"

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== Participants ==
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== Comments and discussion ==
::::# '' We are mostly interested in two WG: (1) Since we are working mostly  with heart and muscles, we are interested in WG 2 - MITOEAGLE data  repository in muscle and other tissues. (2) WG 1 - Standard operating  procedures and user requirement document: Protocols, terminology,  documentation. Since we have an expertise in fatty acid metabolism, we  are specially interested in experimental protocols for the evaluation of  mitochondrial capacities regarding fatty acid metabolism. Of course our  parts of the WG are also in our interest.'' - '''[[Makrecka-Kuka M| Marina Makrecka-Kuka]], [[Liepinsh E|Edgars Liepinsh]]''' (2016).
::::# '' My role in Mito-EAGLE would be mainly in WG 2 and 4, with a focus on  skeletal and cardiac muscle and cultured cardiomyocytes and endothelial  cells. I would particularly like to bridge the gap between the group  leaders and young investigators, trying to represent the young people  who are the active users of the OROBOROS equipment. This will fit in  nicely with the goals in WG1. ... Major congratulations on obtaining  this beautiful collaborative grant! Very glad to see this network  getting the recognition it deserves! Looking forward to being part of  this!'' - '''[[Wuest RC| Rob C Wuest]]''' (2016).
::::# ''Please, find enclosed my declaration to participate with my team in the following workgroups: WG1, WG2, WG3, WG4. We are now involved and have been in not a distant past – in studying of the following cell /tissue types: 
::::## ''Blood cells, with particular emphasis on platelets and lymphocytes – isolated from animals and humans.
::::## ''Endothelial cells, fibroblasts, neurocytes (neuroblastoma), astrocytes, cancer cell lines – predominantly cultured.
::::## ''Hepatocytes, cardiomyocytes, cell isolated from brain – isolated from lab animals. - '''[[Watala C| Cezary Watala]]''' (2016).
::::# ''I have already contacted the portuguese CNC to nominate me for  the MC of theaction. Concerning my active participation, taking into  account the type of work we do in my lab I could be included in all WGs.  As you know we work with mitochondria from liver, muscle and fat, as  well as cultured cells from different origins (WG2-4). The elaboration  of SOPs (WG1) we can certainly contribute (see the book I edited on  Mitochondrial Bioenergetics and in which you contribute a chapter). I  believe that everybody can be part of the WG5, but as the coordinators  of the action, you and Sandra should decide what is best for the success  of the action.'' - '''[[Palmeira C |Carlos Palmeira]]''' (2016).
::::# ''I would propose my participation in the WG1: Standard operating procedures and user requirement document: Protocols, terminology, documentation, considering that the goal of WG1 is to establish an integrated and analytic environment linking mitochondrial respiration with external data (cell physiology, anthropometry, spiroergometry, lifestyle, genomics) in the context of physical fitness evaluation and clinical diagnostics. I could give my active contribution in the phase of making platform that will aggregate the Action’s complete knowledge and provides the ability to make diagnostic profiling associated with diseases in particular tasks:
::::## ''Develop reccomendations for quality control, data reporting and data sharing beyond the published record. Develop concepts on Open Access and institutionalised service for data management, data mining and health-care conforming standards of data interpretation 
::::## ''Provide a summary on strategic dissemination and an education programme for MITOEAGLE.
::::## ''Considering that we are in process of buying the oxygraph, thus broading capacities of my lab, I would be happy to open the door of my new lab for the innovative inter-laboratory  ring test experiments and together with my team contribute in that phase of the MITOEAGLE Action. I look forward to the establishment of MitoGlobal EAGLE network where, as stated in the MoU,  researchers will collaborate on mapping mitochondrial physiology and medicine. The creation of such a unique well-coordinated network of senior researchers and young investigators will definitely put an added value to existing data and publications, will increase quality and validity of the research, will bring new possibilities of data sharing and data mining  and will hopefully bring us to the era of more economical research by saving resources through standardised and harmnonised protocols, where the measuremnts will not be wasted due to lack of comparability.'' - '''[[Lalic Nebojsa|Nebojsa Lalic]]''' (2016).
::::# ''Our research interest is oriented to reproductive endocrinology; especially to mechanisms that govern steroidogenesis of testicular Leydig cells in disturbed homeostasis. We use several in vivo models:'' 
::::## ''stress -/+ systemic or intratesticular blockade of different stress receptors (alpha1ADR; betaADR; GR)
::::## ''hypogonadal-hypogonadism -/+ testosterone-replacement therapy 
::::## ''androgens anabolics treatment -/+ blockade od AR
::::## ''aged rats -/+ Viagra-treatment 
::::## ''biological clock'' - '''[[Andric S|Silvana Andric]]''' (2016).
::::# ''I took a look to the MoU and the different working groups. You know well my research focus and having this into account I am interested in actions related to Working group 1 (Respirometric reference protocols, Interlaboratory proficiency test, Instrumental platforms). Working group 2 and 3: I have generated multiple mitochondrial respiration data sets in different mouse tissues (mainly in skeletal muscle, hypothalamus, liver and white adipose tissue). Currently we are also performing these assays in white adipose tissue from human patients. Hence I am interested in all milestones of WG 2 and 3. WG 5 is related to WG 1 and as you know I have interest in training, dissemination and also together with my collaborators (if needed) we could help with data sets and a data interpretation.  Overall, I have a general interest on this Action and my active role in MITOEAGLE could be discussed. I could be of help participating in some of the tasks where my expertise and interest fit best. We will be forming and interdisciplinary research group with four group leaders: JC Perales, R Guimera, M Sales-Pardo and PM Garcia-Roves with two different affiliations, University of Barcelona (JCP and PMG-R - Energy metabolism) and University Rovira I Virgili (RG and MS – Data base, statistics, data mining).'' - '''[[Garcia-Roves PM|Pablo M Garcia-Roves]]''' (2016).
::::# ''I will be pleased to contribute to the tasks of WG1 by developing interlaboratory proficiency tests and instrumental platform comparison. Moreover, as a (potential) MC member of MitoEAGLE I can also  offer visibility to the Action itself (i.e. WG5: MITOEAGLE dissemination  and training, according to mitoglobal.org webpages)  by establishing  new interactions with the International Coenzyme Q10 Association (ICQA)  where I act as a member of the Executive Committee. ICQA organizes  biennal conferences on  new highlights of Coenzyme Q research. In 2015,  we held the conference in Bologna  (http://conference2015.wix.com/icqaconference) and we included in the  program also some scientific sessions dedicated to mitochondrial  bioenergetics and diseases. I will be pleased to enforce cooperation  between ICQA and MitoEAGLE.'' - '''[[Genova ML| Maria Luisa Genova]]''' (2016).
::::# ''We found the scientific content and objectives of the call very  interesting and compatible with our wide expertise on oxysterol analysis  in food and biological systems. Our group has a large experience on  lipid composition and their degradation products (volatile and  non-volatile), as well as on sensory analysis.    Moreover, we are currently involved in the H2020-GERI-2014-1 project  "PLOTINA" ("Promoting gender balance and inclusion in research,  innovation    and training") with Prof. Gallina Toschi (in cc) as coordinator; the  project actually includes gendered case studies where gender and sex  variables are    considered. You can find further information about our expertises, publications and  the PLOTINA project at the following webpages: https://www.unibo.it/sitoweb/maria.rodriguez/, https://www.unibo.it/sitoweb/tullia.gallinatoschi/, http://www.unibo.it/en/research/projects-and-initiatives/research-projects-horizon-2020-1/plotina'' - '''[[Rodriguez Estrada MT| Maria Teresa Rodriguez Estrada]]''' (2016).
::::# ''As you know, our current/developing interests are with "evolution" (species comparisons) and "environment" (hypoxia and cold exposure), but have also experience and interest in assessing effects of "lifestyle" (diet, exercise) and "aging" as well (in rodents).'' - '''[[Chicco AJ|Adam J Chicco]]''' (2016).
::::# ''Congratulations on the considerable progress with MITOEAGLE! I am keen to participate in this exciting project and I’m sure I can make a valuable contribution to it's success. I have extensive experience performing respirometry assays in human muscle biopsies, myoblasts/tubes and mouse tissues in the contexts of aging, obesity, exercise and inactivity. I feel that I could contribute to any of the five working groups, but particularly to WG2, WG4, and WG5. I am also happy and willing to serve on the Management Committee if needed. Again, congratulations on organizing such an exciting project.'' - '''[[Coen PM|Paul M Coen]]''' (2016).
::::# ''I would be interested to be involved with WG1 and WG2. In particular, I am interested in contributing to developing protocols for multicenter quality control among labs, and for permeabilized muscle bundle respirometry.'' - '''[[Rossiter HB| Harry B Rossiter]]''' (2016).
::::# ''I think that we could contribute via participation in the evaluation of the protocols, and helping to develop them further. In addition, we have been working on the establishing a database that would allow us to keep protocol details, cell isolation and several other aspects. There is nothing very advanced, but we could contribute to establishing such data collection practice. In addition, we, as opposed to many others, happen to use Strathkelvin respirometer. This alternative hardware would allow us to verify whether the protocols work on them as well.'' - '''[[Vendelin M|Marko Vendelin]]''' (2016).

Revision as of 11:46, 17 January 2018