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Difference between revisions of "Talk:Bioblast alert 2020"

From Bioblast
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== In prep ==
== In prep ==
:::: [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]  '''Mitochondrial respiration medium MiR05 wins - KCl inhibits'''


::::::: - from the O2k-Network »[[US TN Nashville Wasserman DH]]« and »[[US MI East Lansing Bazil JN]]«
::::::* Wollenman LC, Vander Ploeg MR, Miller ML, Zhang Y, Bazil JN (2017) The effect of respiration buffer composition on mitochondrial metabolism and function. PLOS ONE 12:e0187523.  - [[Wollenman 2017 PLOS ONE |»Bioblast link«]]
<br />
[[File:CS.png|right|100px|Citrate synthase]]
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| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]
| width="110" align="center" | [[File:O2k-Webinars icon.png|left|80px|MitoPedia]]
| [[Citrate synthase]] activity is commonly applied as a mitochondrial marker - and erroneous applications are common in the literature.
| '''Did you miss the O2k-Webinar on O2k-Applications: simultaneous measurement of O₂ flux and H₂O₂ production presented by [[Komlodi Timea|Dr. Tímea Komlódi]]?''' <br>
- »'''[[MiPNet17.04 CitrateSynthase]]'''«
Watch the recorded presentation. »'''[https://www.youtube.com/watch?v=IJhhvhn_mEM&feature=youtu.be O2k-Webinar: Simultaneous measurement of O<sub>2</sub> and H<sub>2</sub>O<sub>2</sub> production]'''«<br>
Read the follow-up questions and answers. »'''[https://wiki.oroboros.at/index.php/Talk:O2k-Webinars O2k-Webinar Q&A ]'''«
|}
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:{|
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| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
| ETC - the electron transport chain - is the most frequently applied misnomer in bioenergetics. Y Hatefi in the team of DE Green clarified this 58 years ago, introducing the concept of the [[Electron transfer-pathway |electron transfer system]].
| '''Do you know how to select the proper fluorescence sensors and filter set?''' <br>
- »'''[[Hatefi 1962 J Biol Chem-XLII]]'''«
Using different fluorescence sensors, LED and photodiode filters, the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] (or [https://www.oroboros.at/index.php/product/o2k-fluo-led2-module/ O2k-Fluo LED2-Module]) allows for measurement of [[hydrogen peroxide]], [[ATP]], [[mitochondrial membrane potential]], [[calcium]], and numerous other applications open for O2k-user innovation.<br>
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[[O2k-Fluo_LED2-Module#Setup_of_the_O2k-Fluo_LED2-Module|Selecting a Filter Set]]'''«
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::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Physical activity has positive effects on mitochondria from patients with occupational burnout'''
::::::: - from the O2k-Network »[[CH Basel Eckert A]]«
::::::* Brand S, Ebner K, Mikoteit T, Lejri I, Gerber M, Beck J, Holsboer-Trachsler E, Eckert A (2020) Influence of regular physical activity on mitochondrial activity and symptoms of burnout - an interventional pilot study. J Clin Med 9:E667. - [[Brand 2020 J Clin Med|»Bioblast link«]]


== On hold ==
== On hold ==

Revision as of 13:56, 5 August 2020

In prep

MitoPedia
Did you miss the O2k-Webinar on O2k-Applications: simultaneous measurement of O₂ flux and H₂O₂ production presented by Dr. Tímea Komlódi?

Watch the recorded presentation. »O2k-Webinar: Simultaneous measurement of O2 and H2O2 production«
Read the follow-up questions and answers. »O2k-Webinar Q&A «

MitoPedia
Do you know how to select the proper fluorescence sensors and filter set?

Using different fluorescence sensors, LED and photodiode filters, the O2k-Fluo Smart-Module (or O2k-Fluo LED2-Module) allows for measurement of hydrogen peroxide, ATP, mitochondrial membrane potential, calcium, and numerous other applications open for O2k-user innovation.
- »Selecting the Smart Fluo-Sensor« and »Selecting a Filter Set«

On hold