Takahashi 2014 Exp Gerontol: Difference between revisions
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{{Labeling | {{Labeling | ||
|area=Respiration, Genetic knockout;overexpression, Gender | |area=Respiration, Genetic knockout;overexpression, Gender | ||
|diseases=Aging;senescence | |||
|organism=Mouse | |organism=Mouse | ||
|tissues=Skeletal muscle, Liver | |tissues=Skeletal muscle, Liver | ||
|preparations=Isolated mitochondria | |preparations=Isolated mitochondria | ||
|couplingstates=LEAK, OXPHOS | |couplingstates=LEAK, OXPHOS | ||
|pathways=N, NS | |pathways=N, NS | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional=JP, | |||
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Latest revision as of 17:37, 25 February 2020
Takahashi K, Noda Y, Ohsawa I, Shirasawa T, Takahashi M (2014) Extended lifespan, reduced body size and leg skeletal muscle mass, and decreased mitochondrial function in clk-1 transgenic mice. Exp Gerontol 58:146-53. |
Takahashi K, Noda Y, Ohsawa I, Shirasawa T, Takahashi M (2014) Exp Gerontol
Abstract: Mutational inactivation of clk-1, which encodes an enzyme necessary for the biosynthesis of coenzyme Q (CoQ), extends the lifespan of Caenorhabditis elegans. However, whether mammalian clk-1 regulates the lifespan of mice is not known because clk-1-deficiencies are embryonic lethal. Here, we investigated the lifespan of clk-1 transgenic mice (Tg96/I), which were rescued from embryonic lethality via the transgenic expression of mouse clk-1. Tg96/I mice lived longer and had smaller bodies than wild-type mice, but Tg96/I mice had CoQ levels equivalent to wild-type mice. The small-sized Tg96/I mice exhibited reduced whole-body oxygen consumption (VO2) during the dark period, and lean leg skeletal muscles with reduced mitochondrial VO2 and ATP content compared with wild-type mice. These findings indicate a close relationship between lifespan extension and decreased mitochondrial function, which was induced by the transgenic expression of clk-1, in leg skeletal muscles that exhibit high metabolic activity. โข Keywords: Lifespan, Mitochondrial function, clk-1, Coenzyme Q, Oxygen consumption, Leg skeletal muscles
โข O2k-Network Lab: JP Tokyo Tanaka M
Labels: MiParea: Respiration, Genetic knockout;overexpression, Gender
Pathology: Aging;senescence
Organism: Mouse Tissue;cell: Skeletal muscle, Liver Preparation: Isolated mitochondria
Coupling state: LEAK, OXPHOS
Pathway: N, NS
HRR: Oxygraph-2k
JP