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Difference between revisions of "Superoxide dismutase"

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[[File:O2-.jpg|right|60px|Superoxide anion]]
[[File:O2-.jpg|right|60px|Superoxide anion]]
== Application in [[HRFR]] ==
== Application in [[HRR]] ==
::::* Add SOD for conversion of O<sub>2</sub><sup>•-</sup> and [[MiPNet20.14_AmplexRed_H2O2-production|measurement of H<sub>2</sub>O<sub>2</sub> with Amplex ultrared]].
::::* Add SOD for conversion of O<sub>2</sub><sup>•-</sup> and [[MiPNet20.14_AmplexRed_H2O2-production|measurement of H<sub>2</sub>O<sub>2</sub> with Amplex ultrared]].
::::* '''SOD: Superoxide dismutase from bovine liver''', Sigma S8160, 15 KU, store at -20°C; MW = 32.000,00 g/mol


:::: '''SOD: Superoxide dismutase from bovine liver''', Sigma S8409, 15 KU, store at -20°C; MW = 32.000,00 g/mol
::: '''Preparation of 5000 U/mL stock solution''' (dissolved in H<sub>2</sub>O)
 
:::: '''Preparation of 5000 U/ml stock solution''' (dissolved in H<sub>2</sub>O):
 
::: '''Preparation'''


::::# Weigh 2 mg of superoxide dismutase.
::::# Weigh 2 mg of superoxide dismutase.
::::# Dissolve in 1134 µl H<sub>2</sub>O.  
::::# Dissolve in 1134 µL H<sub>2</sub>O.  
::::# Divide into 30 µl portions into 0.2 ml Eppendorf tubes.
::::# Divide into 30 µL portions into 0.2 mL Eppendorf tubes.
::::# Store frozen at -20 °C in the desiccator.
::::# Store frozen at -20 °C in the desiccator.

Revision as of 12:42, 17 March 2019


high-resolution terminology - matching measurements at high-resolution


Superoxide dismutase

Description

Mammalian superoxide dismutase (SOD) exists in three forms, of which the Mn-SOD occurs in mitochondria (mtSOD, SOD2; 93 kD homotetramer) and many bacteria, in contrast to the Cu-Zn forms of SOD (cytosolic SOD1, extracellular SOD3 anchored to the extracellular matrix and cell surface). Superoxide anion (O2•-) is a major reactive oxygen species (ROS) which is dismutated by SOD to oxygen and H2O2.

Abbreviation: SOD

Reference: Fridovich_1997_J Biol Chem


MitoPedia topics: Enzyme 

Superoxide anion

Application in HRR

Preparation of 5000 U/mL stock solution (dissolved in H2O)
  1. Weigh 2 mg of superoxide dismutase.
  2. Dissolve in 1134 µL H2O.
  3. Divide into 30 µL portions into 0.2 mL Eppendorf tubes.
  4. Store frozen at -20 °C in the desiccator.