Difference between revisions of "Superoxide dismutase"

Revision as of 11:15, 22 August 2017

high-resolution terminology - matching measurements at high-resolution

Superoxide dismutase

Description

Mammalian superoxide dismutase (SOD) exists in three forms, of which the Mn-SOD occurs in mitochondria (mtSOD, SOD2; 93 kD homotetramer) and many bacteria, in contrast to the Cu-Zn forms of SOD (cytosolic SOD1, extracellular SOD3 anchored to the extracellular matrix and cell surface). Superoxide anion (O₂^•-) is a major reactive oxygen species (ROS) which is dismutated by SOD to oxygen and H₂O₂.

Abbreviation: SOD

Reference: Fridovich_1997_J Biol Chem

MitoPedia topics: Enzyme

Application in HRFR

Add SOD for conversion of O₂^•- and measurement of H₂O₂ with Amplex ultrared.

SOD: Superoxide dismutase from bovine liver, Sigma S8409, 15 KU, store at -20°C; MW = 32.000,00 g/mol

Preparation of 5000 U/ml stock solution (dissolved in H₂O):

Preparation

Weigh 2 mg of superoxide dismutase.
Dissolve in 1134 µl H₂O.
Divide into 30 µl portions into 0.2 ml Eppendorf tubes.
Store frozen at -20 °C in the desiccator.

@@ Line 4: / Line 4: @@
 |info=[[Fridovich_1997_J Biol Chem]]
 }}
-{{MitoPedia methods}}
 {{MitoPedia topics
 |mitopedia topic=Enzyme
 }}
 [[File:O2-.jpg|right|60px|Superoxide anion]]
-== Application in fluorometry  ==
+== Application in [[HRFR]] ==
-* Add SOD for conversion of O<sub>2</sub><sup>•-</sup> and [[MiPNet20.14_AmplexRed_H2O2-production|measurement of H<sub>2</sub>O<sub>2</sub> with Amplex Red]].
+::::* Add SOD for conversion of O<sub>2</sub><sup>•-</sup> and [[MiPNet20.14_AmplexRed_H2O2-production|measurement of H<sub>2</sub>O<sub>2</sub> with Amplex ultrared]].
+:::: '''SOD: Superoxide dismutase from bovine liver''', Sigma S8409, 15 KU, store at -20°C; MW = 32.000,00 g/mol
-'''SOD:  Superoxide Dismutase from bovine liver''', Sigma S8409, 15 KU, store at -20°C; MW = 32.000,00 g/mol
+:::: '''Preparation of 5000 U/ml stock solution''' (dissolved in H<sub>2</sub>O):
+== Preparation ==
-'''Preparation of 5000U/ml stock solution''' (dissolved in H<sub>2</sub>O):
+::::# Weigh 2 mg of superoxide dismutase.
+::::# Dissolve in 1134 µl H<sub>2</sub>O.
-::1) Weigh 2 mg of Superoxide dismutase
+::::# Divide into 30 µl portions into 0.2 ml Eppendorf tubes.
-::2) Dissolve in 1134µl H<sub>2</sub>O
+::::# Store frozen at -20 °C in the desiccator.
-::3) Divide into 30µl portions into 0.2 ml eppendorf tubes
-::4) Store frozen at -20°C in the Desiccator