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Steady state

From Bioblast


high-resolution terminology - matching measurements at high-resolution


Steady state

Description

A system is in a steady state if the levels of a specific variable (e.g. O2 flux, H2O2 flux, mitochondrial membrane potential) in the system do not change over time. High-resolution respirometry allows for the measurement of different parameters (e.g. O2 flux, H2O2 flux, mitochondrial membrane potential) at a steady state. Combination with the Titration-Injection microPump (TIP2k) allows operation with programmable titration regimes and at quasi steady-states, yielding an expanded flexibility in experimental design by combining the technical advantages of closed and open systems approaches.


Reference: MiPNet06.01_O2k-Overview, Harrison 2015 J Appl Physiol


MitoPedia concepts: "MitoFit Quality Control System" is not in the list (MiP concept, Respiratory state, Respiratory control ratio, SUIT concept, SUIT protocol, SUIT A, SUIT B, SUIT C, SUIT state, Recommended, ...) of allowed values for the "MitoPedia concept" property. MitoFit Quality Control System"MitoFit Quality Control System" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property. 


MitoPedia methods: Respirometry, Fluorometry, Spectrophotometry 


MitoPedia O2k and high-resolution respirometry: DatLab 



Communicated by Doerrier C, 2020-04-20, updated 2020-04-23, 2020-05-28

DatLab oxygen flux: performance and data analysis

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