Difference between revisions of "Starovlah 2019b MiP2019"

Revision as of 14:52, 26 September 2019

Mitochondrial dynamic markers and acrosomal reaction are disturbed in spermatozoa from stressed adult rats.

Link: MiP2019

Starovlah IM, Radovic SM, Kostic TS, Andric SA (2019)

Event: MiP2019

Mitochondria are key component of energy production, oxidative stress, calcium homeostasis, steroid biosynthesis, but also the key component of stress response. However, the molecular adaptation of spermatozoa from stressed males was not described well.

The aim of this study was to determine the functionality and molecular adaptation of spermatozoa from stressed rat by applying in vivo and in vitro approach. For in vivo experimental model, psychophysiological stress by immobilization (IMO), was performed for 3 hours in different time during the day (ZT3, ZT11, ZT23), for one (1xIMO) or ten (10xIMO) consecutive days. For in vitro approach, epididymal spermatozoa from undisturbed rats were stimulated with stress hormones adrenaline and cortisol.

Results showed that number of spermatozoa significantly decreased in all 10xIMO rats comparing to control. Acrosomal status (response to acrosome-reaction-inducer progesterone) significantly decreased in spermatozoa from 1xIMO and 10xIMO rats comparing to control. The same effect was observed in spermatozoa stimulated in vitro with stress hormones. RQ-PCR results revealed that transcription of the main mitochondrial biogenesis markers Ppargc1a, Nrf1 and Nrf2 increased in spermatozoa from 10xIMO rats in ZT3 time point. In the same spermatozoa samples transcription of main markers of mitochondrial architecture Opa1, Mfn1, Mfn2 increased in ZT3 while decreased in ZT11 time point. Incubation of spermatozoa with adrenaline decreased level of Ppargc1a and Nrf2a transcripts, while cortisol decreased expression of mitochondrial transcription factor TFAM.

In summary, repeated psychophysical stress decreased the number and functionality of spermatozoa and disturbed transcriptional profile of their mitochondrial biogenesis and architecture markers.

• Bioblast editor: Plangger M, Tindle-Solomon L

Labels: MiParea: mtDNA;mt-genetics, nDNA;cell genetics, Comparative MiP;environmental MiP

Organism: Rat Tissue;cell: Heart

Affiliations and support

Lab Reproductive Endocrinology Signaling, Lab Chronobiology Aging, Centre Excellence CeRES, Fac Sciences, Univ Novi Sad, Novi Sad, Serbia. - [email protected]

This work was supported by the grant no. 173057 and grant no. 451-0302807 Centre of Excellence CeRES from the Ministry of Education, Science and Technological Development, Republic of Serbia, as well as grant no. 2130 from the Autonomous Province of Vojvodina.

@@ Line 1: / Line 1: @@
 {{Abstract
-|title=[[Image:Isidora Starovlah-MitoEAGLE2019.JPG|left|90px|Isidora Starovlah]] Psychophysiological stress changes transcriptional profile of molecular markers of mitochondrial dynamics in myocardium of adult male rats.
+|title=[[Image:Isidora Starovlah-MitoEAGLE2019.JPG|left|90px|Isidora Starovlah]] Mitochondrial dynamic markers and acrosomal reaction are disturbed in spermatozoa from stressed adult rats.
 |info=[[MiP2019]]
 |authors=Starovlah IM, Radovic SM, Kostic TS, Andric SA
@@ Line 6: / Line 6: @@
 |event=MiP2019
 |abstract=[[Image:MITOEAGLE-logo.jpg|left|100px|link=http://www.mitoglobal.org/index.php/MITOEAGLE|COST Action MitoEAGLE]]
-Stress is an energy dependent and demanded process and requires the mitochondria as mediators representing the site of crossing the convergent pathways. Although psychophysical stress is the most common stress in human society, the markers of mitochondrial dynamics in myocardium were not described.
+Mitochondria are key component of energy production, oxidative stress, calcium homeostasis, steroid biosynthesis, but also the key component of stress response. However, the molecular adaptation of spermatozoa from stressed males was not described well.
-The aim of this study was to determine the transcription profile of main markers of mitochondrial dynamics in myocardium of acutely or repeatedly stressed adult male rats. The psychophysiological stress was performed by immobilization (IMO) for 3 hours for one (1xIMO, acute stress) or ten (10xIMO, repeated stress) consecutive days.
+The aim of this study was to determine the functionality and molecular adaptation of spermatozoa from stressed rat by applying ''in vivo'' and ''in vitro'' approach. For ''in vivo'' experimental model, psychophysiological stress by immobilization (IMO), was performed for 3 hours in different time during the day (ZT3, ZT11, ZT23), for one (1xIMO) or ten (10xIMO) consecutive days. For ''in vitro'' approach, epididymal spermatozoa from undisturbed rats were stimulated with stress hormones adrenaline and cortisol.
-RQ-PCR-results revealed that transcription of PGC1, the master regulator of mitochondrial dynamics and integrator of environmental signals, decreased in myocardium from both, acutely and repeatedly stressed rats comparing to undisturbed controls. The transcripts of PGC1-downstream-targets Nrf1/Nrf2 declined only in 10xIMO group. Tfam significantly increased in hearts of both stressed groups. Markers of mitochondrial fusion/architecture, Mfn1 and Opa1, increased in myocardium from 10xIMO group, while Mfn2 remained unchanged. This was followed by reduction of Mtnd1 (mtDNA encoded transcript whose core subunit belongs to the minimal assembly required for catalysis). In the same samples, the transcription of main markers of mitophagy was opposite: Pink increased, but Prkn decreased. Mitochondrial functional markers (Ucp1, Ucp2, Ucp3) increased in myocardium from 10xIMO-group.
+Results showed that number of spermatozoa significantly decreased in all 10xIMO rats comparing to control. Acrosomal status (response to acrosome-reaction-inducer progesterone) significantly decreased in spermatozoa from 1xIMO and 10xIMO rats comparing to control. The same effect was observed in spermatozoa stimulated ''in vitro'' with stress hormones. RQ-PCR results revealed that transcription of the main mitochondrial biogenesis markers Ppargc1a, Nrf1 and Nrf2 increased in spermatozoa from 10xIMO rats in ZT3 time point. In the same spermatozoa samples transcription of main markers of mitochondrial architecture Opa1, Mfn1, Mfn2 increased in ZT3 while decreased in ZT11 time point. Incubation of spermatozoa with adrenaline decreased level of Ppargc1a and Nrf2a transcripts, while cortisol decreased expression of mitochondrial transcription factor TFAM.
-According to our best knowledge, these results are the first data showing the transcriptional profile of mitochondrial dynamics markers in myocardium of stressed organism and can be used as a solid base for further multidisciplinary research to clarify the molecular mechanisms.
+In summary, repeated psychophysical stress decreased the number and functionality of spermatozoa and disturbed transcriptional profile of their mitochondrial biogenesis and architecture markers.
 |editor=[[Plangger M]], [[Tindle-Solomon L]]
 }}