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Property "Has-20abstract" (as page type) with input value "-5B-5BImage:MITOEAGLE-2Dlogo.jpg-7Cleft-7C100px-7Clink=http:-2F-2Fwww.mitoglobal.org-2Findex.php-2FMITOEAGLE-7CCOST-20Action-20MitoEAGLE-5D-5D-0AIn-20recent-20years-20there-20has-20been-20an-20increasing-20interest-20in-20mitochondrial-20(mt)-20research-20in-20many-20physio-2Dpathological-20conditions.-20In-20this-20context,-20the-20evaluation-20of-20mt-2Drespiration-20is-20crucial-20for-20understanding-20mt-2Dphysiology-20and-20bioenergetics-20in-20health-20and-20disease.-20However,-20despite-20of-20the-20growing-20relevance-20in-20mt-2Dresearch,-20there-20is-20no-20database-20which-20provides-20information-20about-20mt-2Dfunction.-20-5B-5BMitoEAGLE-20network-5D-5D-20provides-20a-20powerful-20tool-20for-20establishing-20a-20useful-20mt-2Ddatabase-20in-20different-20species,-20tissues,-20and-20sample-20preparations.-20However,-20the-20comparison-20of-20reported-20data-20on-20mt-2Drespiration-20from-20the-20scientific-20literature-20and-20inter-2Fintra-2Dlaboratory-20comparisons-20are-20very-20difficult.-20In-20permeabilized-20muscle-20fibers-20(pfi)-20one-20of-20the-20critical-20issues-20comparing-20respiratory-20data-20is-20the-20quality-20of-20the-20mitochondrial-20preparations.-20Therefore,-20the-20generation-20of-20reference-20values-20which-20allow-20the-20evaluation-20of-20the-20skills-20preparing-20high-2Dquality-20pfi,-20constitutes-20one-20of-20the-20main-20aims-20of-20the-20-5B-5BWG2-5D-5D-20(muscle)-20in-20the-20-5B-5BMitoEAGLE-5D-5D-20project.-20For-20this-20purpose,-20as-20a-20first-20step,-20we-20performed-20a-20pilot-20study-20collecting-20mt-2Drespiratory-20data-20(pfi-20from-20mouse-20soleus)-20in-20different-20laboratories-20following-20the-20same-20experimental-20procedure.-20In-20this-20previous-20pilot-20study,-20we-20observed-20heterogeneous-20results-20between-20the-20groups-20involved-20-5B1-5D.-20In-20the-20present-20study,-20we-20addressed-20some-20potential-20aspects-20which-20could-20trigger-20this-20variability.-20-0A-0AMitochondrial-20respiration-20was-20evaluated-20by-20-5B-5Bhigh-2Dresolution-20respirometry-5D-5D-20in-20pfi-20from-20soleus-20muscle-20from-20-27-27N-27-27=15-20C57BL6-2FJ-20male-20mice-20using-20the-20-5B-5BSUIT-2D008-20O2-20pfi-20D014-5D-5D-20protocol-20(Fig.-201)-20-5B2-5D.-20Experiments-20were-20performed-20in-20parallel-20in-20the-20same-20laboratory-20by-20two-20researchers-20from-20different-20research-20groups.-0A-20-0AOur-20results-20did-20not-20exhibit-20significant-20differences-20on-20mitochondrial-20respiration-20by-20comparing-20inspanidual-20mechanical-20sample-20preparations-20and,-20the-20effect-20of-20chemicals-20prepared-20for-20this-20study-20in-20both-20laboratories.-20On-20the-20other-20hand,-20we-20compared-20the-20results-20obtained-20for-20each-20researcher-20in-20the-20former-20and-20present-20study.-20We-20detected-20significant-20differences-20in-20oxygen-20consumption-20between-20both-20studies.-20Our-20findings-20demonstrate-20two-20sources-20of-20variability-20in-20mitochondrial-20respiration.-20First,-20ADP-20storage-20(time-20and-2For-20temperature)-20seems-20to-20be-20a-20critical-20aspect-20affecting-20oxygen-20fluxes.-20Wet-20weight-20is-20the-20other-20important-20issue-20that-20must-20be-20considered-20and-20well-2Dexplained-20for-20generating-20reliable-20results-20in-20the-20future.-20-0A-0AThe-20achievement-20of-20the-20reference-20values-20constitutes-20the-20starting-20point-20for-20obtaining-20reliable-20data-20in-20any-20project,-20avoiding-20an-20under-2Foverestimation-20that-20could-20mask-2Finfluence-20relevant-20results-20in-20a-20study.-20This-20fact-20will-20contribute-20to-20decrease-20and-20maybe-20avoid-20non-2Dreproducible-20data-20between-20different-20laboratories.-20Finally,-20this-20study-20will-20benefit-20the-20elaboration-20of-20a-20public-20mt-2Ddatabase-20in-20muscle-20permeabilized-20tissues." contains invalid characters or is incomplete and therefore can cause unexpected results during a query or annotation process.

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