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- Reverse electron flow from CII to CI + (Reverse electron flow from CII to CI stimu … Reverse electron flow from CII to CI stimulates production of [[ROS]] when mitochondria are incubated with succinate without rotenone in the LEAK state at a high [[mt-membrane potential]]. Depolarisation of the mt-membrane potential (''e.g.'' after ADP addition to stimulate OXPHOS) leads to inhibition of RET and therefore, decrease of RET-initiated ROS production. RET can be also measured when mitochondria are respiring using [[Glycerophosphate |Gp]] without rotenone in the [[LEAK respiration|LEAK]] state. Addition of I<sub>Q</sub>-side inhibitors (ubiquinone-binding side of CI) of [[Complex I |CI]] usually block RET. The following SUIT protocols allow you to measure RET-initiated H<sub>2</sub>O<sub>2</sub> flux in [[mitochondrial preparations]]: [[SUIT-009]] and [[SUIT-026]].[[SUIT-026]].)
