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| {{MitoPedia
| | #REDIRECT [[O2k-Fluo Smart-Module]] |
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| {{MitoPedia O2k and high-resolution respirometry
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| |mitopedia O2k and high-resolution respirometry=O2k hardware
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| __TOC__
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| {{Template:Technical support integrated}}
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| __TOC__
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| == O2k-Guide ==
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| [[File:Smart_Fluo-Sensor_-_connect_.png|thumb|150px|'''Orientation''' for connection]]
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| [[File:Smart_Fluo-Sensor_RIGHT.jpg|thumb|150px|'''OK''', fully inserted]]
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| [[File:Smart_Fluo-Sensor_WRONG.jpg|thumb|150px|'''WRONG''', not fully inserted]]
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| [[File:Smart_Fluo-Sensor_inserted.jpg|thumb|150px|'''Horizontal''' cable routing]]
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| === Select the Smart Fluo-Sensors ===
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| :::: Switching between different excitation wavelengths and filters is achieved by simply exchanging the Smart Fluo-Sensors. Two types of optical sensors are supplied with different LEDs for fluorescence excitation, and the effective spectra of the LEDs are modified by filters.
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| :::: Select the fluorescence sensor and filter set form the table on [[MiPNet17.05_O2k-Fluorescence_LED2-Module#Fluorophores|application-specific settings]]. Each fluorescence sensor is delivered with a mounted filter set:
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| ::::# [[Fluorescence-Sensor Green]]: 525 nm. The installed Filter-Cap is ready for H2O2 measurement with [[Amplex red|Amplex® UltraRed]].
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| ::::# [[Fluorescence-Sensor Blue]]: 465 nm. The installed Filter-Cap is ready for measurements of mt-membrane potential with [[safranin]]. A different filter is used for measurement with [[Magnesium green| Magnesium green®]] or Calcium green®.
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| '''O2k-Guide: Setup - next step [[Filter-Cap#Mounting_a_Filter-Cap|Mounting a Filter-Cap]]'''
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| === Connect Smart Fluo-Sensor to O2k ===
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| :::: Connect the Smart Fluo-Sensor cable to the Fluo plug, insert the male plug of the cable into the female Fluo plug. The red dot on the male plug faces straight upwards. Each Smart Fluo-Sensor can be used on O2k-Chamber A or B. The blue frame of the chamber window and the Smart Fluo-Sensor are specially designed to only connect at a specific orientation, when the unbowed notches align. In this optimal position, the Smart Fluo-Sensor is carefully inserted in the window opening until the sensor completely and gapless covers the chamber window. In this position, the cable routing must be horizontal.
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| :::: To remove the Sensor, carefully pull out the Smart Fluo-Sensor while slightly turning. Do not pull on the cable!
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| === Stoppers ===
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| :::: Use only [[Stopper\black PEEK\conical Shaft\central Port|black PEEK stoppers]] in conjunction with fluorometric measurements. The black stoppers can be used for all HRFR applications in general. See [[MiPNet22.11_O2k-FluoRespirometer_manual]] for calibration of the O2k-chamber volume.
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| <!-- '''O2k-Guide: [[Fluorescence-Control_Unit#O2k-Guide|Electronic settings]]''' -->
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| [[Category:Technical service]]
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