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Difference between revisions of "Sek 2019 ASMRM2019"

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{{Abstract
{{Abstract
|title=Sęk A, Kampa RP, Kulawiak B, Bauer T, Szewczyk A, Bednarczyk P (2019) Localization of the BK<sub>Ca</sub> channel in the inner mitochondria membrane of human bronchial epithelial cell line. ASMRM2019.
|title=Sęk A, Kampa RP, Kulawiak B, Bauer T, Szewczyk A, Bednarczyk P (2019) Localization of the BK<sub>Ca</sub> channel in the inner mitochondrial membrane of human bronchial epithelial cell line. ASMRM2019.
|info=[http://asmrm.org/index.php?gt=meet/meet01 ASMRM 2019]
|info=[http://asmrm.org/index.php?gt=meet/meet01 ASMRM 2019]
|authors=Sek A, Kampa RP, Kulawiak B, Bauer T, Szewczyk A, Bednarczyk P
|authors=Sek A, Kampa RP, Kulawiak B, Bauer T, Szewczyk A, Bednarczyk P
|year=2019
|year=2019
|event=[[Asia Society for Mitochondrial Research and Medicine]]
|event=[[Asia Society for Mitochondrial Research and Medicine]]
|abstract=Mitochondria are dynamic, bioenergetic intracellular organelles composed of two membranes a smooth outer membrane and a markedly folded inner mitochondrial membrane (IMM). In the IMM there are located different proteins such as ion channels mediating ion fluxes. Potassium transport via ion channels into the mitochondrial matrix can protect the injured cardiac and neuronal tissues. This ion transport seems to be a crucial mechanism to control the inner membrane potential, volume of the mitochondrial matrix and the rate of generation of reactive oxygen species. Possibly, activation of mitochondrial large-conductance Ca2+-regulated potassium (mitoBKCa) channel can provide beneficial effects on the epithelia exposed to harmful factors.
|abstract=Mitochondria are dynamic, bioenergetic intracellular organelles composed of two membranes a smooth outer membrane and a markedly folded inner mitochondrial membrane (IMM). In the IMM there are located different proteins such as ion channels mediating ion fluxes. Potassium transport via ion channels into the mitochondrial matrix can protect the injured cardiac and neuronal tissues. This ion transport seems to be a crucial mechanism to control the inner membrane potential, volume of the mitochondrial matrix and the rate of generation of reactive oxygen species. Possibly, activation of mitochondrial large-conductance Ca<sub>2+</sub>-regulated potassium (mitoBK<sub>Ca</sub>) channel can provide beneficial effects on the epithelia exposed to harmful factors.


The aim of studies is the biophysical and pharmacological characterization of potassium channels in the inner mitochondrial membrane of bronchial epithelial cells (16HBE14o-).
The aim of studies is the biophysical and pharmacological characterization of potassium channels in the inner mitochondrial membrane of bronchial epithelial cells (16HBE14o-).


Presence of the BKCa channel in the inner mitochondrial membrane of human bronchial epithelial cells was proved using the patch-clamp technique. The mitoBKCa channel activity was inhibited by paxilline, potassium channel blocker. The channel was activated by Ca2+ and NS11021 known as a specific activator of the BKCa channel. The large conductance potassium channel interactions with flavonoids was also studied.. Additionally, the presence in the inner mitochondrial membrane proteins of pore-forming α subunits and auxiliary β subunits of the BKCa channel was confirmed using the Western Blot analysis. Genes expression of the BKCa channel subunits were studied with the use reverse transcriptase-PCR analysis.
Presence of the BK<sub>Ca</sub> channel in the inner mitochondrial membrane of human bronchial epithelial cells was proved using the patch-clamp technique. The mitoBK<sub>Ca</sub> channel activity was inhibited by paxilline, potassium channel blocker. The channel was activated by Ca<sub>2+</sub> and NS11021 known as a specific activator of the BK<sub>Ca</sub> channel. The large conductance potassium channel interactions with flavonoids was also studied. Additionally, the presence in the inner mitochondrial membrane proteins of pore-forming α subunits and auxiliary β subunits of the BK<sub>Ca</sub> channel was confirmed using the Western Blot analysis. Genes expression of the BK<sub>Ca</sub> channel subunits were studied with the use reverse transcriptase-PCR analysis.


We believe that our results of BKCa channels in mitochondria of human epithelial will allow for better understanding of their role in cytoprotective mechanisms.
We believe that our results of BK<sub>Ca</sub> channels in mitochondria of human epithelial will allow for better understanding of their role in cytoprotective mechanisms.
|editor=[[Plangger M]],
|editor=[[Plangger M]],
}}
}}
{{Labeling}}
{{Labeling
|area=mt-Membrane, Pharmacology;toxicology
|organism=Human
|tissues=Endothelial;epithelial;mesothelial cell
|enzymes=Inner mt-membrane transporter
|topics=Ion;substrate transport
|additional=Flavonoids,
}}
== Affiliations and support ==
== Affiliations and support ==
::::Sek(1,2), Kampa RP(1,3), Kulawiak B(1), Bauer T(2), Szewczyk A(1), Bednarczyk P(3)
::::Sek(1,2), Kampa RP(1,3), Kulawiak B(1), Bauer T(2), Szewczyk A(1), Bednarczyk P(3)

Latest revision as of 06:58, 21 February 2020

Sęk A, Kampa RP, Kulawiak B, Bauer T, Szewczyk A, Bednarczyk P (2019) Localization of the BKCa channel in the inner mitochondrial membrane of human bronchial epithelial cell line. ASMRM2019.

Link: ASMRM 2019

Sek A, Kampa RP, Kulawiak B, Bauer T, Szewczyk A, Bednarczyk P (2019)

Event: Asia Society for Mitochondrial Research and Medicine

Mitochondria are dynamic, bioenergetic intracellular organelles composed of two membranes a smooth outer membrane and a markedly folded inner mitochondrial membrane (IMM). In the IMM there are located different proteins such as ion channels mediating ion fluxes. Potassium transport via ion channels into the mitochondrial matrix can protect the injured cardiac and neuronal tissues. This ion transport seems to be a crucial mechanism to control the inner membrane potential, volume of the mitochondrial matrix and the rate of generation of reactive oxygen species. Possibly, activation of mitochondrial large-conductance Ca2+-regulated potassium (mitoBKCa) channel can provide beneficial effects on the epithelia exposed to harmful factors.

The aim of studies is the biophysical and pharmacological characterization of potassium channels in the inner mitochondrial membrane of bronchial epithelial cells (16HBE14o-).

Presence of the BKCa channel in the inner mitochondrial membrane of human bronchial epithelial cells was proved using the patch-clamp technique. The mitoBKCa channel activity was inhibited by paxilline, potassium channel blocker. The channel was activated by Ca2+ and NS11021 known as a specific activator of the BKCa channel. The large conductance potassium channel interactions with flavonoids was also studied. Additionally, the presence in the inner mitochondrial membrane proteins of pore-forming α subunits and auxiliary β subunits of the BKCa channel was confirmed using the Western Blot analysis. Genes expression of the BKCa channel subunits were studied with the use reverse transcriptase-PCR analysis.

We believe that our results of BKCa channels in mitochondria of human epithelial will allow for better understanding of their role in cytoprotective mechanisms.


Bioblast editor: Plangger M


Labels: MiParea: mt-Membrane, Pharmacology;toxicology 


Organism: Human  Tissue;cell: Endothelial;epithelial;mesothelial cell 

Enzyme: Inner mt-membrane transporter  Regulation: Ion;substrate transport 



Flavonoids 

Affiliations and support

Sek(1,2), Kampa RP(1,3), Kulawiak B(1), Bauer T(2), Szewczyk A(1), Bednarczyk P(3)
  1. Lab Intracellular Ion Channels, Nencki Inst Experimental Biology, Warsaw, Poland
  2. Fac Chemistry, Univ Warsaw, Warsaw, Poland
  3. Dept Biophysics, Warsaw Univ Life Sciences (SGGW), Warsaw, Poland
This study was supported by a grant 2016/21/B/NZ1/02769 from the National Science Centre, Poland (to PB), partially by Nencki Institute. Work implemented as a part of Operational Project Knowledge Education Development 2014-2020 cofinanced by European Social Fund; POWER.03.02.00-00-I007/16-00 (to A. Sęk)