SUIT-020 O2 mt D032: Difference between revisions
No edit summary |
No edit summary |
||
Line 9: | Line 9: | ||
::: '''[[SUIT protocol pattern]]:''' orthogonal 1PM;2D;3G;4S;5Rot;6Omy;7U;8Ama | ::: '''[[SUIT protocol pattern]]:''' orthogonal 1PM;2D;3G;4S;5Rot;6Omy;7U;8Ama | ||
This is a protocol to investigate the N and NS-pathway control state on isolated mitochondria and tissue homogenate. Omy has to be titrated in small amount in order to avoid overtitration and therefore, inhibition of the flux in the ET-state. | This is a protocol to investigate the N and NS-pathway control state on isolated mitochondria and tissue homogenate. Omy has to be titrated in small amount in order to avoid overtitration and therefore, inhibition of the flux in the ET-state. | ||
Originially this is a short protocol for simultaneous determination of O<sub>2</sub> flux and | Originially this is a short protocol for simultaneous determination of O<sub>2</sub> flux and mitochondrial membrane potential on isolated mitochondria and tissue homogenate. It is used as a control experiment for [[SUIT-020 Fluo mt D033]] without using the fluorescent dye in order to detect the effect of it on the O<sub>2</sub> flux as a control. | ||
__TOC__ | __TOC__ |
Revision as of 12:45, 28 January 2019
Description
Abbreviation: NS(PGM)
Reference: A: SUIT-020 short protocol for determination of O2 flux in mitochondrial preparations (isolated mitochondria and tissue homogenate).
SUIT number: D032_1PM;2D;3G;4S;5Rot;6Omy;7U;8Ama
O2k-Application: Fluo
- SUIT-category: NS(PGM)
- SUIT protocol pattern: orthogonal 1PM;2D;3G;4S;5Rot;6Omy;7U;8Ama
This is a protocol to investigate the N and NS-pathway control state on isolated mitochondria and tissue homogenate. Omy has to be titrated in small amount in order to avoid overtitration and therefore, inhibition of the flux in the ET-state. Originially this is a short protocol for simultaneous determination of O2 flux and mitochondrial membrane potential on isolated mitochondria and tissue homogenate. It is used as a control experiment for SUIT-020 Fluo mt D033 without using the fluorescent dye in order to detect the effect of it on the O2 flux as a control.
Communicated by Komlodi T (last update 2019-01-28)
DLP applications
- SUIT-020 Fluo mt D033 simultaneous determination of O2 flux and mt-membrane potential using any fluorescence dye (Safranin, TMRM etc) on isolated mitochondria and tissue homogenate.
- SUIT-020 Fluo mt D036 simultaneous determination of O2 flux and mt-membrane potential using any fluorescence dye (Safranin, TMRM etc) on isolated mitochondria and tissue homogenate.
- SUIT-020 O2 mt D032 determination of O2 flux on isolated mitochondria and tissue homogenate as a control for SUIT-020 Safr mt D033.
- SUIT-020 O2 mt D035 determination of O2 flux on isolated mitochondria and tissue homogenate as a control for SUIT-020 Safr mt D036.
References
Strengths and limitations
- + Information in one protocol about the NS, S and N-pathway control state.
- + This protocol can be used as a control for SUIT-020 Fluo mt D033, SUIT-020 Fluo mt D036.
- + CIV activity and Cytochrome c test can be performed.
- - Omy concentration has to be determined. Higher concentration of Omy than required can inhibit the ET state.
Compare SUIT protocols
MitoPedia concepts: SUIT protocol, SUIT A, Find
MitoPedia methods:
Respirometry