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SUIT-008 O2 pfi D014

From Bioblast


high-resolution terminology - matching measurements at high-resolution


SUIT-008 O2 pfi D014

Description

1PM;2D;3G;4S;5U;6Rot-.png

Abbreviation: NS(PGM)

Reference: A: SUIT-008 protocol in MitoEAGLE project (WG2) for testing the skills preparing permeabilized fibers Lemieux 2017 Sci Rep

SUIT number: D14_1PM;2D;2c;3G;4S;4D;5U;6Rot;7Ama;8AsTm;9Azd

O2k-Application: O2

SUIT-category: NS(PGM)
SUIT protocol pattern: diametral 1PM;2D;3G;4S;5U;6Rot


1PM;2D;2c;3G;4S;5U;6Rot-

1PM;2D;2c;3G;4S;5U;6Rot;7Ama.png 1PM;2D;2c;3G;4S;5U;6Rot;7Ama

SUIT states: 1-2PM(LPc) 3PGM 4-5PGMS(PE) 6S 7ROX
Step Respiratory state Pathway control Entry through electron transfer Complex Comments
1PM PM(L) N CI
2D PM(P) N CI
2D(2c) PM(c) N CI
3G PGM(P) N CI
4S PGMS(P) NS CI&II
5U PGMS(E) NS CI&II
6Rot S(E) S CII
7Ama ROX


1PM;2D;3G;3c;4S;5U;6Rot-

1PM;2D;3G;3c;4S;5U;6Rot;7Ama.png 1PM;2D;3G;3c;4S;5U;6Rot;7Ama

SUIT states: 1-2PM(LP) 3PGM(c) 4-5PGMS(PE) 6S 7ROX
Step Respiratory state Pathway control Entry through electron transfer Complex Comments
1PM PM(L) N CI
2D PM(P) N CI
3G PGM(P) N CI
G(c) PGM(c) N CI Limitation: Cytochrome c should be added after step 2D, such that all OXPHOS states can be compared in the presence of cytochrome c. See 1PM;2D;2c;3G;4S;5U;6Rot-.
4S PGMS(P) NS CI&II
5U PGMS(E) NS CI&II
6Rot S(E) S CII
7Ama ROX


References

 YearReferenceOrganismTissue;cell
Lemieux 2017 Sci Rep2017Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers. Sci Rep 7:2840. doi:10.1038/s41598-017-02789-8MouseHeart


MitoPedia concepts: MiP concept, SUIT protocol, Recommended 


MitoPedia methods: Respirometry