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Difference between revisions of "SUIT-008 O2 pfi D014"

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| SUIT 8_pfi
| SUIT 8_pfi
| SUIT protocol used in the MitoEAGLE project (WG2) for testing the skill preparing permeabilized fibers (pfi)
| SUIT protocol used in the MitoEAGLE project (WG2) for testing the skill preparing permeabilized fibers (pfi)
| style="text-align:center"| [[File:Download1.png|20px|link=http://www.bioblast.at/index.php/File:Pfi_1PM;2D;2c;3G;4S;4D;5U;6Rot;7Ama;8AsTm;9Azd.DLP]]
| style="text-align:center"| [[File:Download1.png|20px|link=http://www.bioblast.at/images/4/4d/Pfi_1PM%3B2D%3B2c%3B3G%3B4S%3B4D%3B5U%3B6Rot%3B7Ama%3B8AsTm%3B9Azd.DLP]]
| style="text-align:center"| work in progress
| style="text-align:center"| [[File:Download1.png|20px|link=http://www.bioblast.at/images/9/9b/Pfi_1PM%3B2D%3B2c%3B3G%3B4S%3B4D%3B5U%3B6Rot%3B7Ama%3B8AsTm%3B9Azd.xlsx]]
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Revision as of 09:42, 26 February 2018


high-resolution terminology - matching measurements at high-resolution


SUIT-008 O2 pfi D014

Description

1PM;2D;3G;4S;5U;6Rot-.png

Abbreviation: NS(PGM)

Reference: A: SUIT 8 is the SUIT protocol used in the MitoEAGLE project (WG2) for testing the skill preparing permeabilized fibers Lemieux 2017 Sci Rep

SUIT number: 8


MitoPedia concepts: SUIT protocol, SUIT A 

SUIT-category: NS(PGM)
SUIT protocol pattern: diametral.


1PM;2D;2c;3G;4S;5U;6Rot-

1PM;2D;2c;3G;4S;5U;6Rot;7Ama.png 1PM;2D;2c;3G;4S;5U;6Rot;7Ama

SUIT states: 1-2PM(LPc) 3PGM 4-5PGMS(PE) 6S 7ROX
Step Respiratory state Pathway control Entry through electron transfer Complex Comments
1PM PM(L) N CI
2D PM(P) N CI
2D(2c) PM(c) N CI
3G PGM(P) N CI
4S PGMS(P) NS CI&II
5U PGMS(E) NS CI&II
6Rot S(E) S CII
7Ama ROX


1PM;2D;3G;3c;4S;5U;6Rot-

1PM;2D;3G;3c;4S;5U;6Rot;7Ama.png 1PM;2D;3G;3c;4S;5U;6Rot;7Ama

SUIT states: 1-2PM(LP) 3PGM(c) 4-5PGMS(PE) 6S 7ROX
Step Respiratory state Pathway control Entry through electron transfer Complex Comments
1PM PM(L) N CI
2D PM(P) N CI
3G PGM(P) N CI
G(c) PGM(c) N CI Limitation: Cytochrome c should be added after step 2D, such that all OXPHOS states can be compared in the presence of cytochrome c. See 1PM;2D;2c;3G;4S;5U;6Rot-.
4S PGMS(P) NS CI&II
5U PGMS(E) NS CI&II
6Rot S(E) S CII
7Ama ROX


SUIT O2 DLP for SUIT 8 (DatLab 7.1)

DL-Protocols (DLP) for substrate-uncoupler-inhibitor titrations (see MitoPedia: SUIT) guide through a sequence of coupling and pathway control states. A specific SUIT protocol can be assigned to O2k-Chamber A or B or both. DL-Protocols for different SUIT protocols can be found in the library of protocols MitoPedia: SUIT. Instrumental DL-protocols are displayed in the DL-Protocol library.
Download instructions.
To start the download click on the Download1.png icon. Depending on your browser you will encounter different download scenarios.
(i) A window opens to select whether to "open file with" or to "save file". Please always choose the option "save file"; opening the file directly will not work due to safety reasons.
(ii) When you click the download icon, some browsers will display the text/content of the .DLP file instead of downloading it. In this case go back to this download page and right-click the download icon and choose "save linked file as" from the context-menu. A window will open to save the file. In some cases the suffix of the file might be wrong (e.g. ".HTML") - you must correct the suffix of the file to ".DLP".
DL-Protocol Description Download - Protocol .DLP Download - Analysis .XLSX
SUIT 8_pfi SUIT protocol used in the MitoEAGLE project (WG2) for testing the skill preparing permeabilized fibers (pfi) Download1.png Download1.png

References

 YearReferenceOrganismTissue;cell
Lemieux 2017 Sci Rep2017Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers. Sci Rep 7:2840. doi:10.1038/s41598-017-02789-8MouseHeart