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Difference between revisions of "SUIT-008 O2 pfi D014"

From Bioblast
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|SUIT number=8
|SUIT number=8
|description=[[File:1PM;2D;3G;4S;5U;6Rot-.png|300px]]
|description=[[File:1PM;2D;3G;4S;5U;6Rot-.png|300px]]
|info='''A''' [[Lemieux 2017 Sci Rep]]
|info='''A: SUIT 8 is the SUIT protocol used in the MitoEAGLE project (WG2) for testing the skill preparing permeabilized fibers''' [[Lemieux 2017 Sci Rep]]
}}
}}
{{MitoPedia concepts
{{MitoPedia concepts
|mitopedia concept=SUIT protocol, SUIT B
|mitopedia concept=SUIT protocol, SUIT A
}}
}}
::: '''[[Categories of SUIT protocols |SUIT-category]]:''' NS(PGM)
::: '''[[Categories of SUIT protocols |SUIT-category]]:''' NS(PGM)

Revision as of 15:39, 7 February 2018


high-resolution terminology - matching measurements at high-resolution


SUIT-008 O2 pfi D014

Description

1PM;2D;3G;4S;5U;6Rot-.png

Abbreviation: NS(PGM)

Reference: A: SUIT 8 is the SUIT protocol used in the MitoEAGLE project (WG2) for testing the skill preparing permeabilized fibers Lemieux 2017 Sci Rep

SUIT number: 8


MitoPedia concepts: SUIT protocol, SUIT A 

SUIT-category: NS(PGM)
SUIT protocol pattern: diametral.

References

 YearReferenceOrganismTissue;cell
Lemieux 2017 Sci Rep2017Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers. Sci Rep 7:2840. doi:10.1038/s41598-017-02789-8MouseHeart


1PM;2D;2c;3G;4S;5U;6Rot-

1PM;2D;2c;3G;4S;5U;6Rot;7Ama.png 1PM;2D;2c;3G;4S;5U;6Rot;7Ama

SUIT states: 1-2PM(LPc) 3PGM 4-5PGMS(PE) 6S 7ROX
Step Respiratory state Pathway control Entry through electron transfer Complex Comments
1PM PM(L) N CI
2D PM(P) N CI
D(c) PM(c) N CI
3G PGM(P) N CI
4S PGMS(P) NS CI&II
5U PGMS(E) NS CI&II
6Rot S(E) S CII
7Ama ROX


1PM;2D;3G;3c;4S;5U;6Rot-

1PM;2D;3G;3c;4S;5U;6Rot;7Ama.png 1PM;2D;3G;3c;4S;5U;6Rot;7Ama

SUIT states: 1-2PM(LP) 3PGM(c) 4-5PGMS(PE) 6S 7ROX
Step Respiratory state Pathway control Entry through electron transfer Complex Comments
1PM PM(L) N CI
2D PM(P) N CI
3G PGM(P) N CI
G(c) PGM(c) N CI Limitation: Cytochrome c should be added after step 2D, such that all OXPHOS states can be compared in the presence of cytochrome c. See 1PM;2D;2c;3G;4S;5U;6Rot-.
4S PGMS(P) NS CI&II
5U PGMS(E) NS CI&II
6Rot S(E) S CII
7Ama ROX