Difference between revisions of "PGM-pathway control state"
m (Cardoso Luiza moved page PGM to PGM-pathway control state: To match nomenclature of other pages: PM-pathway control state, GM-pathway control state) |
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|description=[[File:PGM.jpg|left|200px|PGM]] '''PGM''': [[Pyruvate]] & [[Glutamate]] & [[Malate]]. | |description=[[File:PGM.jpg|left|200px|PGM]] '''PGM''': [[Pyruvate]] & [[Glutamate]] & [[Malate]]. | ||
'''MitoPathway control state:''' [[ N| NADH | '''MitoPathway control state:''' [[ N| NADH electron transfer-pathway state]] | ||
'''SUIT protocol:''' [[SUIT-001]], [[SUIT-012]], [[SUIT-014]] | '''SUIT protocol:''' [[SUIT-001]], [[SUIT-012]], [[SUIT-014]] | ||
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}} | }} | ||
::: ''More details'' | ::: ''More details'' | ||
::::» [[NADH | ::::» [[NADH electron transfer-pathway state]] | ||
::::» [[Additive effect of convergent electron flow]] | ::::» [[Additive effect of convergent electron flow]] | ||
::::» [[Respiratory_complexes#Respiratory_complexes_-_more_than_five |Respiratory complexes - more than five]] | ::::» [[Respiratory_complexes#Respiratory_complexes_-_more_than_five |Respiratory complexes - more than five]] |
Revision as of 14:36, 26 August 2020
Description
PGM: Pyruvate & Glutamate & Malate.
MitoPathway control state: NADH electron transfer-pathway state
SUIT protocol: SUIT-001, SUIT-012, SUIT-014
Pyruvate (P) is oxidatively decarboxylated to acetyl-CoA and CO2, yielding NADH catalyzed by pyruvate dehydrogenase. Malate (M) is oxidized to oxaloacetate by mt-malate dehydrogenase located in the mitochondrial matrix. Condensation of oxaloacate with acetyl-CoA yields citrate (citrate synthase). Glutamate&malate is a substrate combination supporting an N-linked pathway control state, when glutamate is transported into the mt-matrix via the glutamate-aspartate carrier and reacts with oxaloacetate in the transaminase reaction to form aspartate and oxoglutarate. Glutamate as the sole substrate is transported by the electroneutral glutamate-/OH- exchanger, and is oxidized in the mitochondrial matrix by glutamate dehydrogenase to α-ketoglutarate ( 2-oxoglutarate), representing the glutamate-anaplerotic pathway control state. 2-oxoglutarate (α-ketoglutarate) is formed from isocitrate (isocitrate dehydrogenase, from oxaloacetate and glutamate by the transaminase, and from glutamate by the glutamate dehydrogenase.
Abbreviation: PGM
Reference: Gnaiger 2014 MitoPathways - Chapter 5.3
MitoPedia concepts:
SUIT state
PGML
PGM pathway in the LEAK state can be evaluated in the following SUIT protocols:
PGMP
PGM pathway in the OXPHOS state can be evaluated in the following SUIT protocols:
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- DL-Protocol for permeabilized fibers (pfi): SUIT-008 O2 pfi D014
- DL-Protocol for permeabilized cells (pce): SUIT-008 O2 ce-pce D025
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PGME
PGM pathway in the ET state can be evaluated in the following SUIT protocols:
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- DL-Protocol for isolated mitochondria and tissue homogenate (mt):SUIT-001 O2 mt D001
- DL-Protocol for permeabilized fibers (pfi):SUIT-001 O2 pfi D002
- DL-Protocol for permeabilized cells (pce): SUIT-001 O2 ce-pce D003
- DL-Protocol for permeabilized PBMC and PLT: SUIT-001 O2 ce-pce D004
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