Difference between revisions of "OctPM pathway control state"
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'''MitoPathway control state:''' [[FN]] | '''MitoPathway control state:''' [[FN]] | ||
'''SUIT protocol:'''ย [[SUIT-002]] | '''SUIT protocol:'''ย [[SUIT-002]], [[SUIT-005]] | ||
This substrate combination supports N-linked flux which is typically higher than FAO capacity (F/FN<0 in the OXPHOS state). In SUIT-RP1, PMOct is induced after PM(E), to evaluate any additive effect of adding Oct. In SUIT-RP2, FAO OXPHOS capacity is measured first, testing for the effect of increasing malate concentration (compare [[malate anaplerotic pathway control state]], M alone), and pyruvate is added to compare FAO as the background state with FN as the reference state. | This substrate combination supports N-linked flux which is typically higher than FAO capacity (F/FN<0 in the OXPHOS state). In SUIT-RP1, PMOct is induced after PM(E), to evaluate any additive effect of adding Oct. In SUIT-RP2, FAO OXPHOS capacity is measured first, testing for the effect of increasing malate concentration (compare [[malate anaplerotic pathway control state]], M alone), and pyruvate is added to compare FAO as the background state with FN as the reference state. | ||
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::::* DL-Protocol for permeabilized fibers (pfi): [[SUIT-002 O2 pfi D006]] | ::::* DL-Protocol for permeabilized fibers (pfi): [[SUIT-002 O2 pfi D006]] | ||
::::* DL-Protocol for permeabilized cells (pce): [[SUIT-002 O2 pce D007]] | ::::* DL-Protocol for permeabilized cells (pce): [[SUIT-002 O2 pce D007]] | ||
:::*[SUIT-005]] | :::*[[SUIT-005]] | ||
::::* DL-Protocol for permeabilized fibers (pfi):[[SUIT-005 O2 pfi D011]] | ::::* DL-Protocol for permeabilized fibers (pfi):[[SUIT-005 O2 pfi D011]] | ||
Revision as of 14:59, 22 January 2019
Description
OctPM: Octanoylcarnitine & Pyruvate & Malate.
MitoPathway control state: FN
SUIT protocol: SUIT-002, SUIT-005
This substrate combination supports N-linked flux which is typically higher than FAO capacity (F/FN<0 in the OXPHOS state). In SUIT-RP1, PMOct is induced after PM(E), to evaluate any additive effect of adding Oct. In SUIT-RP2, FAO OXPHOS capacity is measured first, testing for the effect of increasing malate concentration (compare malate anaplerotic pathway control state, M alone), and pyruvate is added to compare FAO as the background state with FN as the reference state.
Abbreviation: OctPM
Reference: MiPNet21.06 SUIT reference protocol
MitoPedia concepts:
SUIT state
OctPML
FN pathway in the LEAK state can be evaluated in the following SUIT protocols:
OctPMP
FN pathway in the OXPHOS state can be evaluated in the following SUIT protocols:
-
- DL-Protocol for isolated mitochondria and tissue homogenate (mt): SUIT-002 O2 mt D005
- DL-Protocol for permeabilized fibers (pfi): SUIT-002 O2 pfi D006
- DL-Protocol for permeabilized cells (pce): SUIT-002 O2 pce D007
- DL-Protocol for permeabilized fibers (pfi):SUIT-005 O2 pfi D011
-
OctPME
FN pathway in the ET state can be evaluated in the following SUIT protocols: