Difference between revisions of "OctPM pathway control state"
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[[File:SUIT-Reference-Protocol 1.jpg|700px | | [[File:SUIT-Reference-Protocol 1.jpg|700px |1PM;2D;3U;4G;5S;6Oct;7Rot;8Gp-]]<br /> | ||
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[[File:SUIT-Reference-Protocol 2.jpg|700px |SUIT RP2]]<br /> | [[File:SUIT-Reference-Protocol 2.jpg|700px |SUIT RP2]]<br /> | ||
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Revision as of 12:29, 22 January 2018
- high-resolution terminology - matching measurements at high-resolution
OctPM pathway control state
Description
OctPM: Octanoylcarnitine & Pyruvate & Malate.
MitoPathway control state: FN
SUIT protocol: 1PM;2D;3U;4G;5S;6Oct;7Rot;8Gp- - SUIT RP1, SUIT_FNSGp(PGM)02 - SUIT RP1
This substrate combination supports N-linked flux which is typically higher than FAO capacity (F/FN<0 in the OXPHOS state). In SUIT-RP1, PMOct is induced after PM(E), to evaluate any additive effect of adding Oct. In SUIT-RP2, FAO OXPHOS capacity is measured first, testing for the effect of increasing malate concentration (compare malate anaplerotic pathway control state, M alone), and pyruvate is added to compare FAO as the background state with FN as the reference state.
Abbreviation: OctPM
Reference: MiPNet21.06 SUIT reference protocol
MitoPedia concepts:
SUIT state
OctPM(L)
OctPM(P)
- SUIT_FNSGp(PGM)02_4P: 1D 2Oct 3Mtit&Mc (&McNADH) 4P 5G 6S 7U 8Gp 9Rot 10Ama 11Tm 12Azd
OctPM(E)
- 1PM;2D;3U;4G;5S;6Oct;7Rot;8Gp-_4Oct: 1PM 2D&Dc (&DcNADH) 3U 4Oct 5G 6S 7Rot 8Gp 9Ama 10Tm 11Azd
