OctPGM pathway control state: Difference between revisions
From Bioblast
No edit summary |
No edit summary |
||
Line 6: | Line 6: | ||
'''SUIT protocols:''' [[SUIT-002]] | '''SUIT protocols:''' [[SUIT-002]] | ||
:This substrate combination supports N-linked flux which is typically higher than FAO capacity (F/FN< | :This substrate combination supports N-linked flux which is typically higher than FAO capacity (F/FN<1 in the OXPHOS state). In SUIT-RP1, PMOct is induced after PM(E), to evaluate any additive effect of adding Oct. In SUIT-RP2, FAO OXPHOS capacity is measured first, testing for the effect of increasing malate concentration (compare [[malate anaplerotic pathway control state]], M alone), and pyruvaten and glutamate is added to compare FAO as the background state with FN as the reference state. | ||
|info=[[MiPNet21.06 SUIT reference protocol]] | |info=[[MiPNet21.06 SUIT reference protocol]] | ||
}} | }} | ||
Line 12: | Line 12: | ||
|mitopedia concept=SUIT state | |mitopedia concept=SUIT state | ||
}} | }} | ||
{{MitoPedia methods}} | |||
{{MitoPedia O2k and high-resolution respirometry}} | |||
{{MitoPedia topics}} | |||
::: ''More details'' ย | ::: ''More details'' ย | ||
::::ยป [[FN]] | ::::ยป [[FN]] |
Revision as of 09:36, 23 January 2019
Description
OctPGM: Octanoylcarnitine & Pyruvate & Glutamate & Malate.
MitoPathway control state: FN
SUIT protocols: SUIT-002
- This substrate combination supports N-linked flux which is typically higher than FAO capacity (F/FN<1 in the OXPHOS state). In SUIT-RP1, PMOct is induced after PM(E), to evaluate any additive effect of adding Oct. In SUIT-RP2, FAO OXPHOS capacity is measured first, testing for the effect of increasing malate concentration (compare malate anaplerotic pathway control state, M alone), and pyruvaten and glutamate is added to compare FAO as the background state with FN as the reference state.
Abbreviation: OctPGM
Reference: MiPNet21.06 SUIT reference protocol
MitoPedia concepts:
SUIT state
- More details
OctPGML
FN pathway in the LEAK state can be evaluated in the following SUIT protocols:
OctPGMP
FN pathway in the OXPHOS state can be evaluated in the following SUIT protocols:
-
- DL-Protocol for isolated mitochondria and tissue homogenate (mt): SUIT-002 O2 mt D005
- DL-Protocol for permeabilized fibers (pfi): SUIT-002 O2 pfi D006
- DL-Protocol for permeabilized cells (pce): SUIT-002 O2 pce D007
-
OctPGME
FN pathway in the ET state can be evaluated in the following SUIT protocols: