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Difference between revisions of "O2k-Open Support"

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{{Template:OROBOROS support page name}}
{{Template:OROBOROS support page name}}
 
{{NextGen-O2k H2020-support}}
<big>'''We provide service and support based on open innovation'''</big>     
[[File:O2k-support system.jpg|right|350px|Oroboros support system]]
[[File:O2k-Support.jpg|right|200px|O2k-Open Support]]
:::::::::::: <big>''Currently Asked Questions - »'''[[O2k-Open Support alert]]'''« </big>  
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== O2k-Open Support: technical help ==
== O2k-Open Support: technical help ==
[[File:O2k-support system.jpg|right|350px|Oroboros support system]]
 
:::: '''O2k-Open Support''' includes [[Open Access]] pages that help to optimally use the O2k, to apply instrumental O2k-Quality Control, and perform user-service when needed.
:::: '''O2k-Open Support''' includes [[Open Access]] pages that help to optimally use the O2k, to apply instrumental O2k-Quality Control, and perform user-service when needed. Support regarding data analysis and/or interpretation is provided on an individual basis, for which consultancy fees apply.         
::::::# [[MitoPedia: O2k]]: find information quickly with MitoPedia, the Bioblast glossary with lists of key words and links to relevant pages. (1) [[O2k-Open_Support#MitoPedia:_O2k-Open_Support |O2k-Open Support Glossary]], (2) [[MitoPedia: O2k hardware]], (3) [[MitoPedia: DatLab]]
::::::# '''MitoPedia: O2k''': find information quickly with MitoPedia, the Bioblast glossary with lists of key words and links to relevant pages.
::::::## '''[[MitoPedia: O2k-Open Support]]'''
::::::## [[MitoPedia: O2k hardware]]
::::::## [[MitoPedia: DatLab]]
::::::# [[O2k-Procedures]]: explain various applications of the O2k with O2k-Demo experiments
::::::# [[O2k-Procedures]]: explain various applications of the O2k with O2k-Demo experiments
::::::# [[O2k-Manual]]: topic specific manuals with step by step help
::::::# [[O2k-Manual]]: topic specific manuals with step by step help
::::::# [[O2k-Videosupport]]
::::::# [[O2k-Videosupport]]: provides valuable assistance, complementary to the O2k-Manual
::::::# [[O2k-Products]]: is not merely a 'List of products', it presents a guided tour to the O2k and integrates the concept of [[Gentle Science| Gentle Science and Open Science]].
::::::# [[O2k-Products]]: is not merely a 'List of products', it presents a guided tour to the O2k and integrates the concept of [[Gentle Science| Gentle Science and Open Science]].


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== O2k-Quality Control ==
::: '''<big>O2k-Quality Control</big>'''
[[File:MitoFit-QCS.jpg|right|400px|MitoFit-QCS]]
[[File:MitoFit-QCS.jpg|right|400px|MitoFit-QCS]]
:::: Many commonly raised issues of O2k-technical support are solved in the newest version of [[DatLab]].       
:::: Many commonly raised issues of O2k-technical support are solved in the newest version of [[DatLab]].       


:::: The '''O2 sensor and O2k chamber tests''' are important to eliminate instrumental artefacts (instrumental quality control, QC), distinguished from assay QC addressing problems of [[solution protocols]] (preparation of chemicals), [[sample]] preparation protocols (such as [[mitochondrial preparations]]), and [[SUIT protocols]].  
:::: The '''O<sub>2</sub> sensor and O2k chamber tests''' are important to eliminate instrumental artefacts (instrumental quality control, QC), distinguished from assay QC addressing problems of [[solution protocols]] (preparation of chemicals), [[sample]] preparation protocols (such as [[mitochondrial preparations]]), and [[SUIT protocols]].  
::::# Clean O2k-chambers and contamination-free incubation medium are required.  
::::# Clean O2k-chambers and contamination-free incubation medium are required.  
::::# Analysis of DatLab files recorded in instrumental QC tests is essential.         
::::# Analysis of DatLab files recorded in instrumental QC tests is essential.         
::::# Biological experiments are not suitable for trouble shooting.  
::::# Biological experiments are not suitable for trouble shooting.  


:::: The '''sequence''' of instrumental QC tests is strictly
::::# O2 sensor test
::::# O2k-chamber test       
[[File:O2 sensor test.jpg|right|400px|O2 sensor test|link=MiPNet06.03 POS-calibration-SOP]]
=== O2 sensor test ===


=== 1. O<sub>2</sub> sensor test ===
:::: The [[OroboPOS|OroboPOS]] (polarographic oxygen sensor) requires regular service at intervals - these may be more than 1 year. SOPs are available to determine if a sensor service is required at an earlier or later date:
:::: The [[OroboPOS|OroboPOS]] (polarographic oxygen sensor) requires regular service at intervals - these may be more than 1 year. SOPs are available to determine if a sensor service is required at an earlier or later date:
::::» O2 sensor test: [[Oxygen_sensor_test |'''QC1: Oxygen sensor test''']]       
::::» O<sub>2</sub> sensor test: [[Oxygen_sensor_test |'''QC1: Oxygen sensor test''']]       


=== O2k-chamber test ===
=== 2. O2k-chamber test ===
:::: If all quality control criteria of the O<sub>2</sub> sensor test are met, the operator can be assured that the quality of the sensor signal is acceptable. Next, the quality of the O2k-chamber assembly has to be tested, described in detail:
::::» O2k-chamber test: [[Oxygen flux - instrumental background |'''QC2: Instrumental O<sub>2</sub> background test''']]     


:::: If all quality control criteria of the O2 sensor test are met, the operator can be assured that the quality of the sensor signal is acceptable. Next, the quality of the O2k-chamber assembly has to be tested, described in detail:
::::» O2k-chamber test: [[Oxygen flux - instrumental background |'''QC2: Instrumental O<sub>2</sub> background''']]     


== O2k-Open Support: Installation and startup support session ==
:::::» '''[[MiPNet26.07 Installation and startup support session]]'''


=== Search for defective components: the 2-chamber design of the O2k ===


:::: The '''two chamber design''' of the O'''2k''' has many benefits, including advantages in '''trouble shooting'''. By switching components between sides A and B of the O2k, it is usually possible to locate an instrumental problem, finding selectively a specific defective component of the O2k. This component can then be serviced by the end user or replaced by Oroboros without the need to ship the entire O2k.     
:::: Upon special request and subject to an additional fee, Oroboros Instruments offers a start-up visit by a scientific application specialist.
:::::» '''[[O2k On-site Training]]'''




== O2k-Open Support by Oroboros experts ==
[[File:O2k-Support.jpg|right|200px|O2k-Open Support]]
 
== O2k-Open Support agreement ==
::::* O2k-Open Support is given to individual users by Oroboros experts through our [[O2k-ticket system]].  
::::* We welcome and value feedback. Technical and scientific questions, which are not yet addressed in the available guidelines or are difficult to trace on our website, are relevant to many specialists and beginners in the field.
::::* O2k-Open Support aims at sharing our support communication openly with the scientific community, (''1'') improving the scope and documentation of the O2k-technology, and (''2'') providing [[Open Access]] educational examples on answering questions and finding solutions - in the spirit of [[Gentle_Science|Gentle Science]].
::::* O2k-Open Support by Oroboros experts does not replace the obligation to consult the [[O2k-Manual|O2k-Manual]] and relevant chapters on [[O2k-Procedures]] prior to contacting the O2k-support team.


=== O2k-Open Support agreement ===
:::: The Oroboros experts will be happy to help in using our O2k-Open Support system.
::::*O2k-Open Support aims at sharing our support communication openly with the scientific community.
::::» [[Search for defective O2k components]]
::: We would like to publish your latest question and example files on our website. You can decide if your name, institutional and Email address are included.
:::: '''»''' In case you need further technical support and for your valuable feedback, please contact [https://www.oroboros.at/index.php/o2k-technical-support '''O2k-Tech Support''']     
::: Kindly have a look at http://wiki.oroboros.at/index.php/O2k-Open_Support#O2k-Open_Support_by_Oroboros_experts and give us your confirmation via return email.
::::» [[O2k-Open Support agreement]]
::: Please note that regular consultancy fees might apply, should you not agree with our O2k-Open Support agreement.
::::» [[O2k repair]]
::: We will be happy to assist you - in the spirit of Gentle Science ( http://www.bioblast.at/index.php/Gentle_Science ).
 
:::: [[File:O2k-Open Support procedure.png|left|700px|O2-Open Support]]
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=== Repair ===
 
:::: Hardware problems may require replacement of a defective spare part. Some electronic or mechanical defects may be solved only by repair of the O2k in the electronics workshop of Oroboros Instruments, ''e.g.'', a defective Peltier unit.
 
:::: A cost estimation for repair of the O2k-Main Unit due to an electronic or mechanical defect can only be given after inspection of the O2k in our electronics workshop in Austria. Once a cost estimation has been provided, the repair is carried out with the customer’s consent. For shipment of the O2k, follow the [[Shipping_an_oxygraph|detailed instructions]].     




[[File:O2k-Network.png|left|100px|O2k-Network|link=O2k-Network]]
[[File:O2k-Network.png|left|100px|O2k-Network|link=O2k-Network]]
== The O2k-Network ==
== The O2k-Network ==


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::::: '''»''' [[O2k-Network_discussion_forum|O2k-Network discussion forum]]       
::::: '''»''' [[O2k-Network_discussion_forum|O2k-Network discussion forum]]       


 
:::: '''»''' [[Oroboros Laboratory|Oroboros O2k-Laboratory]] - open for innovation and cooperation.  
== Contact ==
 
:::: '''»''' In case you need further technical support and for your valuable feedback, please contact [mailto:[email protected]| [email protected]]     
 
:::: '''»''' [[Oroboros_MitoFit_Laboratory|Oroboros MitoFit Laboratory]] - open for innovation and cooperation.  
:::: '''»''' [[Oroboros_Science_Scholarship|Oroboros Science Scholarships]] - are offered for focused research projects on mitochondrial physiology and high-resolution respirometry       
:::: '''»''' [[Oroboros_Science_Scholarship|Oroboros Science Scholarships]] - are offered for focused research projects on mitochondrial physiology and high-resolution respirometry       
== MitoPedia: O2k-Open Support ==
{{#ask:[[MitoPedia O2k and high-resolution respirometry::O2k-Open Support]]
| mainlabel=Term
|?Has abbr=Abbreviation
|?Description=Description
|format=broadtable
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::: <big>'''Search further keywords for O2k-Open Support'''</big> » [[:Category:O2k-Open Support |Alphabetical list of keywords]]


[[Category:O2k-Open Support]]
[[Category:O2k-Open Support]]

Latest revision as of 15:43, 9 August 2022


                  


O2k-Open Support

O2k-Open Support


Template NextGen-O2k.jpg
Oroboros support system
Currently Asked Questions - »O2k-Open Support alert« 

O2k-Open Support: technical help

O2k-Open Support includes Open Access pages that help to optimally use the O2k, to apply instrumental O2k-Quality Control, and perform user-service when needed. Support regarding data analysis and/or interpretation is provided on an individual basis, for which consultancy fees apply.
  1. MitoPedia: O2k: find information quickly with MitoPedia, the Bioblast glossary with lists of key words and links to relevant pages.
    1. MitoPedia: O2k-Open Support
    2. MitoPedia: O2k hardware
    3. MitoPedia: DatLab
  2. O2k-Procedures: explain various applications of the O2k with O2k-Demo experiments
  3. O2k-Manual: topic specific manuals with step by step help
  4. O2k-Videosupport: provides valuable assistance, complementary to the O2k-Manual
  5. O2k-Products: is not merely a 'List of products', it presents a guided tour to the O2k and integrates the concept of Gentle Science and Open Science.


O2k-Quality Control
MitoFit-QCS
Many commonly raised issues of O2k-technical support are solved in the newest version of DatLab.
The O2 sensor and O2k chamber tests are important to eliminate instrumental artefacts (instrumental quality control, QC), distinguished from assay QC addressing problems of solution protocols (preparation of chemicals), sample preparation protocols (such as mitochondrial preparations), and SUIT protocols.
  1. Clean O2k-chambers and contamination-free incubation medium are required.
  2. Analysis of DatLab files recorded in instrumental QC tests is essential.
  3. Biological experiments are not suitable for trouble shooting.


1. O2 sensor test

The OroboPOS (polarographic oxygen sensor) requires regular service at intervals - these may be more than 1 year. SOPs are available to determine if a sensor service is required at an earlier or later date:
» O2 sensor test: QC1: Oxygen sensor test

2. O2k-chamber test

If all quality control criteria of the O2 sensor test are met, the operator can be assured that the quality of the sensor signal is acceptable. Next, the quality of the O2k-chamber assembly has to be tested, described in detail:
» O2k-chamber test: QC2: Instrumental O2 background test


O2k-Open Support: Installation and startup support session

» MiPNet26.07 Installation and startup support session


Upon special request and subject to an additional fee, Oroboros Instruments offers a start-up visit by a scientific application specialist.
» O2k On-site Training


O2k-Open Support

O2k-Open Support agreement

The Oroboros experts will be happy to help in using our O2k-Open Support system.
» Search for defective O2k components
» In case you need further technical support and for your valuable feedback, please contact O2k-Tech Support
» O2k-Open Support agreement
» O2k repair


O2k-Network

The O2k-Network

The Oroboros O2k-Network serves to connect and support: Contact for advice on applications of the O2k and high-resolution respirometry - the global network in mitochondrial physiology.
» The O2k-Network Labs: » O2k-Network
» O2k-Network discussion forum
» Oroboros O2k-Laboratory - open for innovation and cooperation.
» Oroboros Science Scholarships - are offered for focused research projects on mitochondrial physiology and high-resolution respirometry