Difference between revisions of "O2k-Open Support"

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{{Template:OROBOROS support page name}}
 
{{Template:OROBOROS support page name}}
 
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[[File:O2k-support system.jpg|right|350px|Oroboros support system]]
 
<big>'''We provide service and support based on open innovation'''</big>       
 
<big>'''We provide service and support based on open innovation'''</big>       
[[File:O2k-Support.jpg|right|200px|O2k-Open Support]]
 
 
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== O2k-Open Support: technical help ==
 
== O2k-Open Support: technical help ==
[[File:O2k-support system.jpg|right|350px|Oroboros support system]]
 
 
:::: '''O2k-Open Support''' includes [[Open Access]] pages that help to optimally use the O2k, to apply instrumental O2k-Quality Control, and perform user-service when needed.
 
:::: '''O2k-Open Support''' includes [[Open Access]] pages that help to optimally use the O2k, to apply instrumental O2k-Quality Control, and perform user-service when needed.
::::::# '''MitoPedia: O2k''': find information quickly with MitoPedia, the Bioblast glossary with lists of key words and links to relevant pages. (1) [[O2k-Open_Support#MitoPedia:_O2k-Open_Support |O2k-Open Support Glossary]], (2) [[MitoPedia: O2k hardware]], (3) [[MitoPedia: DatLab]]
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::::::# '''MitoPedia: O2k''': find information quickly with MitoPedia, the Bioblast glossary with lists of key words and links to relevant pages.
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::::::## '''[[MitoPedia: O2k-Open Support]]'''
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::::::## [[MitoPedia: O2k hardware]]
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::::::## [[MitoPedia: DatLab]]
 
::::::# [[O2k-Procedures]]: explain various applications of the O2k with O2k-Demo experiments
 
::::::# [[O2k-Procedures]]: explain various applications of the O2k with O2k-Demo experiments
 
::::::# [[O2k-Manual]]: topic specific manuals with step by step help
 
::::::# [[O2k-Manual]]: topic specific manuals with step by step help
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::::::# [[O2k-Products]]: is not merely a 'List of products', it presents a guided tour to the O2k and integrates the concept of [[Gentle Science| Gentle Science and Open Science]].
 
::::::# [[O2k-Products]]: is not merely a 'List of products', it presents a guided tour to the O2k and integrates the concept of [[Gentle Science| Gentle Science and Open Science]].
  
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== O2k-Quality Control ==
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::: '''<big>O2k-Quality Control</big>'''
 
[[File:MitoFit-QCS.jpg|right|400px|MitoFit-QCS]]
 
[[File:MitoFit-QCS.jpg|right|400px|MitoFit-QCS]]
 
:::: Many commonly raised issues of O2k-technical support are solved in the newest version of [[DatLab]].       
 
:::: Many commonly raised issues of O2k-technical support are solved in the newest version of [[DatLab]].       
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:::: The '''sequence''' of instrumental QC tests is strictly  
 
:::: The '''sequence''' of instrumental QC tests is strictly  
::::# O2 sensor test
 
::::# O2k-chamber test       
 
 
[[File:O2 sensor test.jpg|right|400px|O2 sensor test|link=MiPNet06.03 POS-calibration-SOP]]
 
 
=== O2 sensor test ===
 
  
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=== 1. O2 sensor test ===
 
:::: The [[OroboPOS|OroboPOS]] (polarographic oxygen sensor) requires regular service at intervals - these may be more than 1 year. SOPs are available to determine if a sensor service is required at an earlier or later date:
 
:::: The [[OroboPOS|OroboPOS]] (polarographic oxygen sensor) requires regular service at intervals - these may be more than 1 year. SOPs are available to determine if a sensor service is required at an earlier or later date:
 
::::» O2 sensor test: [[Oxygen_sensor_test |'''QC1: Oxygen sensor test''']]       
 
::::» O2 sensor test: [[Oxygen_sensor_test |'''QC1: Oxygen sensor test''']]       
  
=== O2k-chamber test ===
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=== 2. O2k-chamber test ===
 
 
 
:::: If all quality control criteria of the O2 sensor test are met, the operator can be assured that the quality of the sensor signal is acceptable. Next, the quality of the O2k-chamber assembly has to be tested, described in detail:  
 
:::: If all quality control criteria of the O2 sensor test are met, the operator can be assured that the quality of the sensor signal is acceptable. Next, the quality of the O2k-chamber assembly has to be tested, described in detail:  
 
::::» O2k-chamber test: [[Oxygen flux - instrumental background |'''QC2: Instrumental O<sub>2</sub> background''']]       
 
::::» O2k-chamber test: [[Oxygen flux - instrumental background |'''QC2: Instrumental O<sub>2</sub> background''']]       
  
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[[File:O2k-Support.jpg|right|200px|O2k-Open Support]]
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== O2k-Open Support agreement ==
  
=== Search for defective components: the 2-chamber design of the O2k ===
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:::: The Oroboros experts will be happy to help in using our O2k-Open Support system.
 
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::::» [[Search for defective O2k components]]
:::: The '''two chamber design''' of the O'''2k''' has many benefits, including advantages in '''trouble shooting'''. By switching components between sides A and B of the O2k, it is usually possible to locate an instrumental problem, finding selectively a specific defective component of the O2k. This component can then be serviced by the end user or replaced by Oroboros without the need to ship the entire O2k.     
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:::: '''»''' In case you need further technical support and for your valuable feedback, please contact [mailto:support@oroboros.at| support@oroboros.at]     
 
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::::» [[O2k-Open Support agreement]]
 
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::::» [[O2k repair]]
== O2k-Open Support by Oroboros experts ==
 
 
 
::::* O2k-Open Support is given to individual users by Oroboros experts through our [[O2k-ticket system]].
 
::::* We welcome and value feedback. Technical and scientific questions, which are not yet addressed in the available guidelines or are difficult to trace on our website, are relevant to many specialists and beginners in the field.
 
::::* O2k-Open Support aims at sharing our support communication openly with the scientific community, (''1'') improving the scope and documentation of the O2k-technology, and (''2'') providing [[Open Access]] educational examples on answering questions and finding solutions - in the spirit of [[Gentle_Science|Gentle Science]].
 
::::* O2k-Open Support by Oroboros experts does not replace the obligation to consult the [[O2k-Manual|O2k-Manual]] and relevant chapters on [[O2k-Procedures]] prior to contacting the O2k-support team.
 
 
 
=== O2k-Open Support agreement ===
 
::::*O2k-Open Support aims at sharing our support communication openly with the scientific community.
 
::: We would like to publish your latest question and example files on our website. You can decide if your name, institutional and Email address are included.
 
::: Kindly have a look at http://wiki.oroboros.at/index.php/O2k-Open_Support#O2k-Open_Support_by_Oroboros_experts and give us your confirmation via return email.
 
::: Please note that regular consultancy fees might apply, should you not agree with our O2k-Open Support agreement.
 
::: We will be happy to assist you - in the spirit of Gentle Science ( http://www.bioblast.at/index.php/Gentle_Science ).
 
 
 
:::: [[File:O2k-Open Support procedure.png|left|700px|O2-Open Support]]
 
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=== Repair ===
 
 
 
:::: Hardware problems may require replacement of a defective spare part. Some electronic or mechanical defects may be solved only by repair of the O2k in the electronics workshop of Oroboros Instruments, ''e.g.'', a defective Peltier unit.
 
 
 
:::: A cost estimation for repair of the O2k-Main Unit due to an electronic or mechanical defect can only be given after inspection of the O2k in our electronics workshop in Austria. Once a cost estimation has been provided, the repair is carried out with the customer’s consent. For shipment of the O2k, follow the [[Shipping_an_oxygraph|detailed instructions]].     
 
  
  
 
[[File:O2k-Network.png|left|100px|O2k-Network|link=O2k-Network]]
 
[[File:O2k-Network.png|left|100px|O2k-Network|link=O2k-Network]]
 
 
== The O2k-Network ==
 
== The O2k-Network ==
  
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::::: '''»''' [[O2k-Network_discussion_forum|O2k-Network discussion forum]]       
 
::::: '''»''' [[O2k-Network_discussion_forum|O2k-Network discussion forum]]       
 
 
== Contact ==
 
 
:::: '''»''' In case you need further technical support and for your valuable feedback, please contact [mailto:support@oroboros.at| support@oroboros.at]     
 
  
 
:::: '''»''' [[Oroboros_MitoFit_Laboratory|Oroboros MitoFit Laboratory]] - open for innovation and cooperation.  
 
:::: '''»''' [[Oroboros_MitoFit_Laboratory|Oroboros MitoFit Laboratory]] - open for innovation and cooperation.  
 
:::: '''»''' [[Oroboros_Science_Scholarship|Oroboros Science Scholarships]] - are offered for focused research projects on mitochondrial physiology and high-resolution respirometry       
 
:::: '''»''' [[Oroboros_Science_Scholarship|Oroboros Science Scholarships]] - are offered for focused research projects on mitochondrial physiology and high-resolution respirometry       
 
 
== MitoPedia: O2k-Open Support ==
 
 
{{#ask:[[MitoPedia O2k and high-resolution respirometry::O2k-Open Support]]
 
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|?Has abbr=Abbreviation
 
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::: <big>'''Search further keywords for O2k-Open Support'''</big> » [[:Category:O2k-Open Support |Alphabetical list of keywords]]
 
  
 
[[Category:O2k-Open Support]]
 
[[Category:O2k-Open Support]]

Revision as of 11:23, 10 November 2019

OROBOROS INSTRUMENTS

OROBOROS support
        MitoPedia: O2k-Open SupportGlossary&FAQ         MitoPedia: O2k hardwareO2k−hardware         MitoPedia: DatLabDatLab         O2k-Procedures         O2k-Manual         O2k-Videosupport         O2k-Products        


O2k-Open Support

Oroboros support system

We provide service and support based on open innovation

O2k-Open Support: technical help

O2k-Open Support includes Open Access pages that help to optimally use the O2k, to apply instrumental O2k-Quality Control, and perform user-service when needed.
  1. MitoPedia: O2k: find information quickly with MitoPedia, the Bioblast glossary with lists of key words and links to relevant pages.
    1. MitoPedia: O2k-Open Support
    2. MitoPedia: O2k hardware
    3. MitoPedia: DatLab
  2. O2k-Procedures: explain various applications of the O2k with O2k-Demo experiments
  3. O2k-Manual: topic specific manuals with step by step help
  4. O2k-Videosupport
  5. O2k-Products: is not merely a 'List of products', it presents a guided tour to the O2k and integrates the concept of Gentle Science and Open Science.


O2k-Quality Control
MitoFit-QCS
Many commonly raised issues of O2k-technical support are solved in the newest version of DatLab.
The O2 sensor and O2k chamber tests are important to eliminate instrumental artefacts (instrumental quality control, QC), distinguished from assay QC addressing problems of solution protocols (preparation of chemicals), sample preparation protocols (such as mitochondrial preparations), and SUIT protocols.
  1. Clean O2k-chambers and contamination-free incubation medium are required.
  2. Analysis of DatLab files recorded in instrumental QC tests is essential.
  3. Biological experiments are not suitable for trouble shooting.
The sequence of instrumental QC tests is strictly

1. O2 sensor test

The OroboPOS (polarographic oxygen sensor) requires regular service at intervals - these may be more than 1 year. SOPs are available to determine if a sensor service is required at an earlier or later date:
» O2 sensor test: QC1: Oxygen sensor test

2. O2k-chamber test

If all quality control criteria of the O2 sensor test are met, the operator can be assured that the quality of the sensor signal is acceptable. Next, the quality of the O2k-chamber assembly has to be tested, described in detail:
» O2k-chamber test: QC2: Instrumental O2 background
O2k-Open Support

O2k-Open Support agreement

The Oroboros experts will be happy to help in using our O2k-Open Support system.
» Search for defective O2k components
» In case you need further technical support and for your valuable feedback, please contact support@oroboros.at
» O2k-Open Support agreement
» O2k repair


O2k-Network

The O2k-Network

The Oroboros O2k-Network serves to connect and support: Contact for advice on applications of the O2k and high-resolution respirometry - the global network in mitochondrial physiology.
» The O2k-Network Labs: » O2k-Network
» O2k-Network discussion forum
» Oroboros MitoFit Laboratory - open for innovation and cooperation.
» Oroboros Science Scholarships - are offered for focused research projects on mitochondrial physiology and high-resolution respirometry