NextGen-O2k Scientific developments
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The revolutionary all-in-one instrument to conquer mitochondrial disease.
WP2: Scientific developments
Abstract
- The aim of this workpackage is to test measurement conditions for the newly developed modules and the development of specific applications.
- Determination of optimal Q-sensor measurement conditions
- Determination of optimal measurement conditions for the PB-Module
- Development of protocols for scientific, pre-clinical and algal biotechnology applications
- Data handling and quality control
- The aim of this workpackage is to test measurement conditions for the newly developed modules and the development of specific applications.
Achievements
- Project Year 1: During testing in the first project year, optimal measurement conditions were determined for using the Q-sensor with a variety of biological samples. Simultaneously testing began of the beta version of DatLab. New SUIT protocols were developed for general, pre-clinical, and algal biotechnology applications. A focus has also been given to internal and external quality control measures.
- Project Year 2: In the second year of the project, experiments with microalgae began, including determining the process for permeabilization and SUIT protocol development. Two preprints were published on microalgae as a result of this work. A further preprint was published on data from Q-Module experiments about the coupling and pathway control of coenzyme Q redox state and respiration. In addition to these developments, NADH-Module testing also started.
Progress and next steps
See » Track record
Luiza Cardoza, PhD. testing the Q-Module and DatLab8
Microalgae for testing PB module
NextGen-O2k MitoFit Preprints
| Link | View | Reference | Year |
|---|---|---|---|
| Donnelly 2023 MitoFit | MitoFit Preprints 2023.2.  | Donnelly C, Komlódi T, Cecatto C, Cardoso LHD, Compagnion AC, Matera A, Tavernari D, Zanou N, Kayser B, Gnaiger E, Place N (2023) Functional hypoxia reduces mitochondrial calcium uptake. MitoFit Preprints 2023.2. https://doi.org/10.26124/mitofit:2023-0002 — 2024-11-17 published in Redox Biol. | 2023 |
| Went 2021 MitoFit PB | MitoFit Preprints 2021.05.  | Went N, Di Marcello M, Gnaiger E (2021) Oxygen dependence of photosynthesis and light-enhanced dark respiration studied by High-Resolution PhotoRespirometry. MitoFit Preprints 2021.05. https://doi.org/10.26124/mitofit:2021-0005 | 2021 |
| Huete-Ortega 2020 MitoFit Preprint Arch EA | MitoFit Preprint Arch EA20.1. | Huete-Ortega M, Di Marcello M, Iglesias-Gonzalez J, Gnaiger E (2020) High-resolution respirometry for chloroplast and mitochondrial bioenergetics in Chlamydomonas reinhardtii ― towards biotechnology exploitations. https://doi.org/10.26124/mitofit:ea20.algaeurope.0001 | 2020 |
