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Mitochondrial membrane potential

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Revision as of 12:59, 5 October 2012 by Gnaiger Erich (talk | contribs) (Created page with "{{MitoPedia |abbr=mt-membrane potantial }} {{MitoPedia methods}} {{MitoPedia topics}} This page is a guide to various wiki.oroboros.at pages that complement [http://www.oroboros....")
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high-resolution terminology - matching measurements at high-resolution


Mitochondrial membrane potential

Description

Abbreviation: mt-membrane potantial




This page is a guide to various wiki.oroboros.at pages that complement [1] with up-to-date information and discussions. The general manual for the OROBOROS Ion Selective Electrode system (ISE System) is in [MiPNet15.03]. While working with the potentiometric (pX) channel of the O2k, the general guidelines for avoiding damage to the oxygraph by ESD should be followed.

A rudimentary protocol for measuring mitochondrial membrane potential (MiPNet14.05), its mathematical appendix and the most up-to-date spreadsheet templates, DatLab templates, and DatLab demo files, can be found here.

Before measuring membrane potential via TPP+ is even started, the TPP+ inhibitory effect in the studied system should be explored.How to get from the measured TPP+ concentration to the mitochondrial membrane potential: Calculation of mitochondrial membrane potential from measurements with a TPP electrode. This covers also the influence of Unspecific binding.

Practical difficulties in applying the method to permeabilized fibers are discussed in Mitochondrial membrane potential of permeabilized fibers. Some tips for Cleaning the TPP+ electrodesSome important performance parameters of the TPP electrode are summarized on this poster. In any way we hope you will join us for one of our TPP special interest group during an International Oxygraph Course.