Difference between revisions of "Mitochondrial membrane potential"
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Revision as of 22:32, 9 September 2018
- high-resolution terminology - matching measurements at high-resolution
Mitochondrial membrane potential
Description
The mitochondrial membrane potential, mtMP, is the electric part of the protonmotive force, ΞpH+.
ΞΟ = ΞpH+ - ΞΒ΅H+ / F
mtMP or ΞΟ is the potential difference across the inner mitochondrial (mt) membrane, expressed in the electric unit of volt [V]. Electric force of the mitochondrial membrane potential is the electric energy change per βmotiveβ electron or per electron moved across the transmembrane potential difference, with the number of βmotiveβ electrons expressed in the unit coulomb [C].
The chemical part of the protonmotive force, Β΅H+ / F stems from the difference of pH across the mt-membrane. It contains a factor that bridges the gap between the electric force [J/C] and the chemical force [J/mol]. This factor is the Faraday constant, F, for conversion between electric force expressed in joules per coulomb or Volt [V=J/C] and chemical force with the unit joules per mole or Jol [Jol=J/mol],
F = 96.4853 kJol/V = 96,485.3 C/mol
Abbreviation: mtMP, ΞΟ
Reference: Mitchell 1961 Nature, Gnaiger 2014 Preface MiP2014
Communicated by Gnaiger E 2012-10-05, edited 2016-02-06, 2017-09-05.
Template:Keywords Membrane potential
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
Different methods for measurements of mt-membrane potential
- mt-Membrane potential can either be measured in the O2k-FluoRespirometer fluorometrically by using the fluorophores TMRM or Safranin, or potentiometrically with the O2k-TPP+ ISE-Module electrode by using the ion reporter TPP+. All mentioned ion indicator molecules inhibit respiration, which makes it essential to test the optimum concentration.
High-resolution respirometry and mt-membrane potential
- The O2k-MultiSensor system provides a potentiometric and a fluorometric module for measurement of the mt-membrane potential.
- Β» O2k-Manual TPP: MiPNet15.03 O2k-MultiSensor-ISE
- Β» TPP: O2k-technical_support
- Β» MiPNet20.13 Safranin mt-membranepotential
- Β» TMRM: O2k-technical_support
MitoPedia concepts:
Respiratory state,
Recommended,
Ergodynamics
MitoPedia methods:
Respirometry,
Fluorometry
MitoPedia topics:
EAGLE
