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MitoPedia: SUIT A

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high-resolution terminology - matching measurements at high-resolution


MitoPedia: SUIT A

The MitoPedia terminology is developed continuously in the spirit of Gentle Science.


MitoFit
In the Library of SUIT protocols
ยป MitoPedia: SUIT - SUIT concept and a list of all SUIT protocols in the library of SUIT protocols.
ยปA: MitoPedia: SUIT A - you are here: these are SUIT protocols used for cohort studies.
ยปB: MitoPedia: SUIT B - these are published SUIT protocols considered less suitable for cohort studies.
ยปC: MitoPedia: SUIT C - these are SUIT protocols used for exploratory research.
See also:
ยป O2k-Protocols: O2k-Demo experiments



Library of SUIT protocols: SUIT A

SUIT codeSUIT numberSUIT categoryCoupling/pathway control diagramO2k-ApplicationReference
SUIT-001 O2 ce-pce D003D003_ce1;1Dig;1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10AsTm;11AzdRP1 ce-pceSUIT-RP1O2A - SUIT-001 - SUIT reference protocol Reference protocol RP1 for living cells (ce, ROUTINE respiration) and cells permeabilized in the respirometric chamber (pce)
SUIT-001 O2 ce-pce D004D004_ce1;1Dig;1PM;2D;2c;3U;4G;5S;(6Oct);7Rot;8Gp;9Ama;10AsTm;11AzdRP1 ce-pce bloodSUIT-RP1 for PBMC and PLTO2A - SUIT-001 - SUIT reference protocol Reference protocol RP1 for living PBMC and PLT cells (ce, ROUTINE respiration) and cells that are permeabilized in the chamber (pce)
SUIT-001 O2 mt D001D001_mt;1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10AsTm;11AzdRP1 mtSUIT-RP1O2SUIT-001 - SUIT reference protocol Reference protocol RP1 for isolated mitochondria, tissue homogenate and permeabilized cells
SUIT-001 O2 pfi D002D002_pfi;1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10AsTm;11AzdRP1 pfiSUIT-RP1O2SUIT-001 - SUIT reference protocol Reference protocol RP1 for permeabilized fibers
SUIT-002 O2 ce-pce D007D007_ce1;1Dig;1D;2M;3Oct;3c;4M;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13AzdRP2 ce-pceSUIT-RP2O2A - SUIT-002 - SUIT reference protocol Reference protocol RP2 for living cells (ce, ROUTINE respiration) and cells that are permeabilized in the chamber (pce)
SUIT-002 O2 ce-pce D007aD007a_ce1;1Dig;1D;2M;3Oct;3c;4M;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13AzdRP2 ce-pce bloodSUIT-RP2 for PBMC and PLTO2A - SUIT-002 - SUIT reference protocol Reference protocol RP2 for for living PBMC and PLT cells (ce, ROUTINE respiration) and cells that are permeabilized in the chamber (pce)
SUIT-002 O2 mt D005D005_mt;1D;2M;3Oct;3c;4M;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13AzdRP2 mt1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd.pngO2A - SUIT-002 - SUIT reference protocol Reference protocol RP2 for isolated mitochondria, tissue homogenate and permeabilized cells
SUIT-002 O2 pfi D006D006_pfi;1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13AzdRP2 pfiSUIT-RP2O2A -SUIT-002 - SUIT reference protocol Reference protocol RP2 for permeabilized fibers (pfi)
SUIT-003 AmR ce D058D058_ce1;ce1SOD;ce1HRP;ce1AmR;ce2Omy;ce3U;ce4Rot;ce5AmaAmR effect on ceCe1;ce1SOD;ce1HRP;ce1AmR;ce2Omy;ce3U;ce4Rot;ce5Ama.pngAmRB: Coupling control in intact cells with oligomycin to study the inhibitory effect of the AmR assay components on the respiration ; ce: non-permeabilized cells -SUIT-003
SUIT-003 O2 ce D009D009_ce1;ce2Omy;ce3U;ce4AmaCCP-ce shortCe1;ce2Omy;ce3U-.pngO2A: - SUIT-003 - Coupling-control protocol (CCP) in living cells (ce) with oligomycin (LEAK state)
SUIT-003 O2 ce D037D037_ce1;ce1Glc;(ce2Omy);ce3U;ce4AmaCCP-ce Crabtree_RCe1;ce1Glc;ce2(Omy);ce3U;ce4Ama.pngO2A SUIT-003 - Coupling-control protocol and Crabtree effect
SUIT-003 O2 ce D038D038_ce1;(ce2Omy);ce3U;ce3Glc;ce3'U;ce4AmaCCP-ce Crabtree_ECe1;ce2(Omy);ce3U;ce3Glc;ce3'U;ce4Ama.pngO2A Coupling-control protocol and Crabtree effect test -SUIT-003 complex cell coupling-control protocol
SUIT-003 O2 ce D039D039_ce1;(ce2Omy);ce3U;ce4Rot;ce5AmaCCP-ce microalgaeCe1;(ce2Omy);ce3U;ce4Rot;ce5Ama.jpgO2A: Coupling control in intact cells without oligomycin; ce: non-permeabilized cells -SUIT-003
SUIT-003 O2 ce D050D050_ce1;ce1Snv;(ce2Omy);ce3U;ce4Rot;ce5AmaCCP-ce SnvCe1;ce1Snv;(ce2Omy);ce3U;ce4Rot;ce5Ama.pngO2A: - Coupling-control protocol for living cells with plasma membrane-permeable succinate (MitoKit-CII/Succinate-nv) - SUIT-003 - Plasma membrane-permeable succinate (NV118) in ROUTINE respiration. Coupling-control protocol (CCP) in living cells (ce)
SUIT-003 O2 ce-pce D020D020_ce1;ce1P;ce2Omy;ce3U;ce4Rot;ce5S;1Dig;1c;2Ama;3AsTm;4AzdCCVPCe1;ce1P;ce2Omy;ce3U;ce4Rot;ce5S;1Dig;1c;2Ama;3AsTm;4Azd.pngO2A SUIT-003 - Coupling-control protocol and respirometric cell viability test; ce: living cells, pce: permeabilized cells
SUIT-003 pH ce D067D067_ce1;ce1Glc;ce2Omy;ce3DG;ce4AmaCCP-Crabtree with glycolysis inhibitionSUIT-003 O2 ce D067 diagram.pngO2A Coupling-control protocol and Crabtree effect with glycolysis inhibition- SUIT-003
SUIT-004 O2 pfi D010D010_1PM;2D;2c;3U;4S;5Rot;6Ama;7AsTm;8AzdRP1-short pfi1PM;2D;2c;3U;4S;5Rot;6Ama;7AsTm;8Azd.pngO2SUIT-004 - Short protocol linked to SUIT-001 O2 pfi D002 SUIT RP1 (human skeletal muscle)
SUIT-005 O2 pfi D011D011_1OctM;2D;2c;3P;4S;5U;6Rot;7Ama;8AsTm;9AzdRP2-short pfi1OctM;2D;2c;3P;4S;5U;6Rot;7Ama;8AsTm;9Azd.pngO2A: - SUIT-005 short protocol linked to SUIT-002 O2 pfi D006 - SUIT RP2 (human skeletal muscle)
SUIT-006 AmR mt D048D048_1PM;2D;3Omy;4U;5AmaCCP mt PM - AmR1PM;2D;3Omy;4U;5Ama.pngAmRA: short protocol for simultaneous determination of O2 flux and H2O2 in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells)- SUIT-006
SUIT-006 Fluo mt D034D034_1PM;2D;3Omy;4U;5AmaCCP mt PM - Fluo1PM;2D;3Omy;4U;5Ama.pngFluoA: short protocol for simultaneous determination of O2 flux and mitochondrial membrane potential in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells) -SUIT-006
SUIT-006 MgG ce-pce D085D085_ce1;1Dig;1PM;2D;3Cat;4U;5AmaCCP MgG ce-pceCe1;1Dig;1PM;2D;3Cat;4U;5Ama.pngMgGA: short coupling-control protocol for simultaneous determination of O2 flux and mitochondrial ATP production in permeabilized cells -SUIT-006
SUIT-006 MgG mt D055D055_1PM;2D;3Cat;4U;5AmaCCP MgG mt1PM;2D;3Cat;4U;5Ama.pngMgGA: short coupling-control protocol for simultaneous determination of O2 flux and mitochondrial ATP production in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells) -SUIT-006
SUIT-006 O2 ce-pce D029D029_ce1;1Dig;1PM;2D;2c;3Omy;4U;5AmaCCP ce-pce PMCe1;1Dig;1PM;2D;2c;3Omy;4U;5Ama.pngO2A to analyse the coupling control on permeabilized cells in N(PM) pathway control state- SUIT-006
SUIT-006 O2 mt D022D022_1SRot;2D;2c;(3Omy);4U;5AmaCCP mt S(Rot)1SRot;2D;2c;3(Omy);4U;5Ama.pngO2A - SUIT-006 - Protocol for coupling control assessment in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells)
SUIT-006 O2 mt D047D047_1PM;2D;2c;3Omy;4U;5AmaCCP mt PM1PM;2D;2c;3Omy;4U;5Ama.pngO2A SUIT-006 - is a coupling-control protocol in the N-pathway for isolated mitochondria, tissue homogenate and cells permeabilized before addition to the O2k chamber.
SUIT-006 Q ce-pce D073D073_ce1;1Dig;1Q2;1Rot;1S;2D;(3Omy);4U;5Anox;6Ama.pngCCP ce-pce S(Rot)Ce1;1Dig;1Q2;1Rot;1S;2D;(3Omy);4U;5Anox;6Ama.pngQA Coupling control protocol to analyze mitochondrial O2 consumption and the redox state of the Q-pool in S(Rot)-pathway control state in permeabilized cells- SUIT-006
SUIT-006 Q mt D071D071_mt;1Q2;1Rot;1S;2D;(3Omy);4U;5Anox;6Ama.pngCCP mt S(Rot)Mt;1Q2;1Rot;1S;2D;(3Omy);4U;5Anox;6Ama.pngQA Coupling-control protocol to analyze mitochondrial O2 consumption and the redox state of the Q-pool in S(Rot)-pathway control state in isolated mitochondria- SUIT-006
SUIT-007 O2 ce-pce D030D030_ce1;1Dig;1G;2D;2c;3M;4U;5AmaGlutamate anaplerotic pathwayCe1;1Dig;1G;2D;2c;3M;4U;5Ama.pngO2A pce: permeabilized cells - SUIT-007
SUIT-008 O2 ce-pce D025D025_ce1;1Dig;1PM;2D;2c;3G;4S;5U;6Rot;7Ama;8AsTm;9AzdQ-junction ce-pceCe1;1Dig;1PM;2D;2c;3G;4S;5U;6Rot;7Ama;8AsTm;9Azd.pngO2A: for cells-permeabilized cells Lemieux 2017 Sci Rep - SUIT-008
SUIT-008 O2 mt D026D026_1PM;2D;2c;3G;4S;4D;5U;6Rot;7Ama;8AsTm;9AzdQ-junction mtprep1PM;2D;2c;3G;4S;5U;6Rot;7Ama;8AsTm;9Azd.pngO2A: SUIT protocol for mt; mt: isolated mitochondria and tissue homogenate - SUIT-008
SUIT-008 O2 pfi D014D014_1PM;2D;2c;3G;4S;4D;5U;6Rot;7Ama;8AsTm;9Azd1PM;2D;2c;3G;4S;5U;6Rot;7Ama;8AsTm;9Azd.pngO2A - Lemieux 2017 Sci Rep - SUIT-008- O2 pfi D014 protocol was used a a reference protocol in MitoEAGLE project (WG2) to evaluate the researcher skills preparing permeabilized fibers
SUIT-010 O2 ce-pce D008D008_ce1;ce2Rot;ce3S;ce4D;1Dig;1cDig titration-pceRespirometric test of optimum digitonin concentrationO2A -SUIT-010 - Optimum digitonin concentration for pce
SUIT-011 O2 pfi D024D024_1GM;2D;2c;3S;4U;5Rot;6AmaNS physiological maximum capapcity in fibres1GM;2D;2c;3S;4U;5Rot;6Ama.pngO2A pfi: permeabilized fibers - SUIT-011
SUIT-012 O2 ce-pce D052D052_ce1;1Dig;1PM;2D;2c;3G;4U;5AmaN(PGM)Ce1;1Dig;1PM;2D;2c;3G;4U;5Ama.pngO2A Linear coupling control with NADH-linked substrates for pce - SUIT-012
SUIT-012 O2 mt D027D027_1PM;2D;2c;3G;4U;5AmaN CCP mtprep1PM;2D;2c;3G;4U;5Ama.pngO2A Linear coupling control with NADH-linked substrates for isolated mitochondria, tissue homogenate and permeabilized cells- SUIT-012
SUIT-013 AmR ce D023D023_ce1O2 dependence of H2O2 production ceSUIT013 AmR ce D023.pngAmRA SUIT-013
SUIT-014 O2 pfi D042D042_1GM;2D;2c;3P;4S;5U;6Rot;7AmaNS(PGM)1GM;2D;2c;3P;4S;5U;6Rot;7Ama.pngO2A SUIT-014
SUIT-015 O2 pti D043D043_1OctM;2D;2c;3G;4P;5S;6U;7Rot;8AmaFNS(Oct,PGM)1OctM;2D;3G;4P;5S;6U;7Rot;8Ama.pngO2A pti: permeabilized tissue- SUIT-015
SUIT-016 O2 pfi D044D044_1OctM;2D;3G;4S;5Rot;6Omy;7U;8AmaFNS(Oct,GM)1OctM;2D;3G;4S;5Rot;6Omy;7U;7c;8Ama.pngO2A pfi: permeabilized fibers- SUIT-016
SUIT-017 O2 mt D046D046_1OctM;2D;2c;3G;3c;4S;5U;6Rot;7AmaFNS(Oct,GM)1OctM;2D;2c;3G;4S;5U;6Rot;7Ama.pngO2A: SUIT-017
SUIT-017 O2 pfi D049D049_1OctM;2D;2c;3G;4S;5U;6Rot;7AmaFNS(Oct,GM)1OctM;2D;2c;3G;4S;5U;6Rot;7Ama.pngO2A: SUIT-017
SUIT-018 AmR ce-pce D068D068_ce1;1Dig;1GMS;2D;3AmaO2 dependence-H2O2Ce1;1Dig;2GMS;3D;4Ama.pngAmRA -SUIT-018 - SUIT protocol for simultaneous determination of O2 and H2O2 flux in permeabilized cells at various oxygen concentrations
SUIT-018 AmR mt D031D031_mt;1GMS;2D;3AmaO2 dependence-H2O21GMS;2D;3Ama.pngAmRA -SUIT-018 - SUIT protocol for simultaneous determination of O2 and H2O2 flux in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells) at low oxygen concentration (tissue normoxia)
SUIT-018 AmR mt D040D040_mt;1GMS;2D;3AmaNS(GM)SUIT-018AmRA: simultaneous determination of O2 and H2O2 flux in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells)-SUIT-018
SUIT-018 AmR mt D041D041_mt;1GMS;2D;3AmaO2 dependence-H2O21GMS;2D;3Ama.pngAmRA -SUIT-018
SUIT-019 O2 pfi D045D045_1PalM;2D;2c;3Oct;4P;5G;6U;7S;8Rot;9AmaFNS(PalOct,PGM)1PalM;2D;2c;3Oct;4P;5G;6U;7S;8Rot;9Ama.pngO2A SUIT-019
SUIT-020 Fluo mt D033D033_1PM;2D;3G;4S;5Rot;6Omy;7U;8AmaNS(PGM)1PM;2D;3G;4S;5Rot;6Omy;7U;8Ama.pngFluoA:short protocol for simultaneous determination of O2 flux and mitochondrial membrane potential in mitochondrial preparations (isolated mitochondria, permeabilized cells, and tissue homogenate)- SUIT-020
SUIT-020 O2 mt D032D032_1PM;2D;2c;3G;4S;5Rot;6Omy;7U;8AmaQ-junction additivity and respiratory control for membrane potential1PM;2D;2c;3G;4S;5Rot;6Omy;7U;8Ama.pngO2A: short protocol for determination of O2 flux in mitochondrial preparations (isolated mitochondria, permeabilized cells, and tissue homogenate)-SUIT-020
SUIT-021 Fluo mt D036D036_1GM;2D;3S;4Rot;5Omy;6U;7AmaNS(GM)1GM;2D;3S;4Rot;5Omy;6U;7Ama.pngFluoA: short protocol for simultaneous determination of O2 flux and mitochondrial membrane potential in mitochondrial preparations (isolated mitochondria, permeabilized cells, and tissue homogenate)-SUIT-021
SUIT-021 O2 mt D035D035_1GM;2D;2c;3S;4Rot;5Omy;6U;7AmaNS(GM)1GM;2D;2c;3S;4Rot;5Omy;6U;7Ama.pngO2A:short protocol for simultaneous determination of O2 flux and mitochondrial membrane potential in mitochondrial preparations (isolated mitochondria, permabilized cells, and tissue homogenate)-SUIT-021
SUIT-022 O2 ce D051D051_ce1;ce2KCN;ce3SHAMAOX-ce CN+SHAMCe1;ce2KCN;ce3SHAM.v2.png Ce1;ce2KCN;ce3SHAM.pngO2A Determination of the respiration due to the alternative oxidase pathway -SUIT-022
SUIT-023 O2 ce D053D053_ce1;ce2SHAM;ce3KCNAOX-ce SHAM+CNCe1;ce2SHAM;ce3KCN.pngO2A Determination of the respiration due to the cytochrome c oxidase pathway -SUIT-023
SUIT-024 O2 ce-pce D056D056_ce1;1Dig;1PM;2T;2D;3Omy;4AmaN(PM)Ce1;1Dig;1PM;2T;2D;3Omy;4Ama.pngO2Determination of the presence of ATPases in mitochondrial preparations - SUIT-024
SUIT-025 O2 mt D057D057_1D;2M;3Oct;3c;4M;5P;6G;7S;8Rot;9AmaFNS(Oct,PGM)1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Rot;9Ama.pngO2 - SUIT-025
SUIT-026 AmR ce-pce D087D087_ce1;1Dig;1S;2Rot;3D;4AmaRETCe1;1Dig;1S;2Rot;3D;4Ama.pngAmRA - SUIT-026 - Protocol for the evaluation of ROS production by RET in permeabilized cells
SUIT-026 AmR mt D064D064_1S;2Rot;3D;4AmaRETSUIT-026 AmR mt D064.pngAmRA - SUIT-026 - Protocol for the evaluation of ROS production by RET in mtprep
SUIT-026 AmR mt D077D077_1S;2Rot;3D;4AmaRETSUIT-026 AmR mt D064.pngAmRA - SUIT-026 - Protocol for the evaluation of ROS production by RET in mtprep
SUIT-027 O2 ce-pce D065D065_ce1;1Dig;1M;2D;3M;4P;5GMalate anaplerosisCe1;1Dig;1M;2D;3M;4P;5G;6Ama.pngO2A - SUIT-027 - Protocol for analysis of the malate anaplerotic pathway
SUIT-029 O2 mt D066D066_mt;1PM;2T;2D;2c;3Omy;4U;5G;6S;6U;7Rot;8AmaQC_imt_PM_T+OXPHOS+c+Omy_ET_G+S+Rot1PM;2T;2D;2c;3Omy;4U;5G;6S;6U;7Rot;8Ama.pngO2A: quality control evaluation of mitochondrial preparations - SUIT-029
SUIT-031 O2 ce-pce D079D079_ce1;1Dig;1PM;2D;2c;3S;4Rot;5U;6AmaPM+S+RotCe1;1Dig;1PM;2D;2c;3S;4Rot;5U;6Ama.pngO2A to analyse O2 flux in the NS- pathway control state in permeabilized cells- SUIT-031
SUIT-031 O2 mt D075D075_1mt;1PM;2D;2c;3S;4Rot;5U;6AmaPM+S+Rot1PM;2D;2c;3S;4Rot;5U;6Ama.pngO2A to analyse O2 flux in the NS- pathway control state on isolated mitochondria- SUIT-031
SUIT-031 Q ce-pce D074D074_ce1;1Dig;1Q2;1PM;2D;3S;4Rot;5U;6Anoxia;7AmaPM+S+RotCe1;1Dig;1Q2;1PM;2D;3S;4Rot;5U;6Anox;7Ama.pngQA to analyse O2 flux and the Q-redox state in the NS- pathway control state on permeabilized cells- SUIT-031
SUIT-031 Q mt D072D072_mt;1Q2;1PM;2D;3S;4Rot;5U;6Anox;7AmaPM+S+RotMt;1Q2;1PM;2D;3S;4Rot;5U;6Anox;7Ama.pngQA to analyse O2 flux and the Q-redox state in the N-,NS- and S- pathway control state on isolated mitochondria- SUIT-031