COST Action proposal: EAGLE
The objective of the EAGLE project is to improve our knowledge on mitochondrial function in health and disease related to Evolution, Age, Gender, Lifestyle and Environment. Every study of mitochondrial (mt) function and disease is faced with EAGLE as the essential background conditions characterizing the individual patient, subject, study group, species, tissue or even cell line. To address the complex interrelationships of EAGLE with an initial focus on humans and mouse models, the network will enhance the value of each individual study by data sharing and analysis beyond the published record. Highlighting the topic of gender and mitochondrial function, unique new information will emerge from the development of a European database. Protocols, technologies and standard procedures will be compared and strategies defined for improvement of quality control. An interlaboratory ring test will be established as a world-wide innovation in the field of mitochondrial respiratory physiology. The expertise gained and new standards developed will be integrated into a strategic dissemination and education programme for mitochondrial phenotyping, aiming at an expanding European and global EAGLE network and database on mitochondrial physiology and medicine, complementary to established mtDNA databases.
Working groups (WG)
WG1. EAGLE data base on gender and mt-function in health and disease
- Mainly published data from WG participants will be shared in the detail beyond the published record (anonymised data on individuals rather than group averages; tabulated results rather than only figures; conversion to common SI units; comparability of normalization). Measures of mitochondrial respiratory function in human and mouse tissues, blood cells and cell lines will be structured according to the EAGLE background, with specific emphasis on gender-specific mt-function and gender medicine.
WG2. Challenges on quality control for sharing data in mitochondrial physiology
- Basic concepts and communication: Consolidation of concepts and nomenclature on mitochondrial respiratory states and control parameters.Laboratory standards: Protocols, technologies and standard procedures; data base on laboratory protocols.Data sharing in mitochondrial physiology: Quality control, documentation, bioinformatics standards.
WG3. Interlaboratory test and outreach
- An interlaboratory ring test will be introduced in practice as a world-wide innovation in the field of mitochondrial respiratory physiology: design and implementation; reference sample dissemination; test protocol(s); data analysis; joint publication.Comparison of results between and within instrumental platforms.Logistics for extended future collaboration in Europe, North and South America, Asia, Australia and Africa.
WG4. Training and data sharing
- Recommendations for quality control, data reporting and data sharing beyond the published record.Concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation.Summary on strategic dissemination and education programme for MitoGlobal EAGLE.
Erich Gnaiger, Ao.Univ.-Prof., Ph.D.
Medical University of Innsbruck
Department of Visceral, Transplant and Thoracic Surgery
D. Swarovski Research Laboratory
A-6020 Innsbruck, Austria
Disclosure: Dr Erich Gnaiger is founder and CEO of OROBOROS INSTRUMENTS – www.oroboros.at
COST EAGLE Consortium
COST Member States
- Austria, Belgium, Bosnia and Herzegovina, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, The Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey, United Kingdom and the former Yugoslav Republic of Macedonia.
- COST cooperating state: Israel.