Difference between revisions of "MiPNet15.03 O2k-MultiSensor-ISE"

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|description='''Ion selective electrodes (ISE)''' are components of O2k-MultiSensor-ISE Modules for measurement of mt-membrane potential (TPP<sup>+</sup>; [[safranin]]), [[calcium]], or [[pH]] simulaneously with O<sub>2</sub> concentration and respiration.
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::::» [[Tetraphenylphosphonium#O2k-technical_support |TPP: O2k-technical_support mt-membrane potential]]
 
::::» [[Tetraphenylphosphonium#O2k-technical_support |TPP: O2k-technical_support mt-membrane potential]]
 
::::» [[Calcium#O2k-technical_support |Calcium: O2k-technical_support]]
 
::::» [[Calcium#O2k-technical_support |Calcium: O2k-technical_support]]
::::» Other ions beside TPP+ TPMP+, and Ca2+: There are ion selective membranes available, e.g. from Sigma Aldrich, for a wide variety of species. If cut to a circle with a diameter of 4 mm these membranes can be used in the ISE system. Many ions may also be detected by fluorimetric based methods.
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::::» Other ions beside TPP<sup>+</sup>, TPMP<sup>+</sup>, and Ca<sup>2+</sup>: There are ion selective membranes available, ''e.g.'', from Sigma Aldrich, for a wide variety of ionic species. If cut to a circle with a diameter of 4 mm these membranes can be used in the ISE system. Many ions may also be detected by fluorimetric based methods.
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== Keywords: Force and membrane potential ==
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{{Keywords Force and membrane potential}}
  
 
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Revision as of 14:20, 7 November 2019

Oroboros Instruments

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MiPNet15.03 O2k-MultiSensor-ISE

Publications in the MiPMap
O2k-Manual
O2k-MultiSensor system with ion selective electrodes (ISE).

» Bioblast pdf »Versions

Oroboros (2018-01-25) Mitochondr Physiol Network

Abstract: Fasching M, Gnaiger E (2018) O2k-MultiSensor system with ion selective electrodes (ISE). Mitochondr Physiol Network 15.03(08):1-26. -

O2k-Manual: The ion selective electrode (ISE) is a modular O2k-MultiSensor extension of the Oroboros O2k. ISE yield a potentiometric (voltage) signal simultaneously with the oxygen signal in both chambers of the O2k. The ISE system consists of separate reference and measuring electrodes selective for hydrophobic cations (TPP+, TPMP+), Ca2+, Mg2+, etc. This O2k-Manual describes the handling and application of the ISE system.

» Product: O2k-TPP+ ISE-Module, Oroboros O2k-Catalogue

Keywords: HRR, O2k-MultiSensor

O2k-Network Lab: AT_Innsbruck_Oroboros


Description

Ion selective electrodes (ISE) are components of O2k-MultiSensor-ISE Modules for measurement of mt-membrane potential (TPP+; safranin), calcium, or pH simulaneously with O2 concentration and respiration.

Template NextGen-O2k.jpg
O2k-Open Support


MitoPedia O2k and high-resolution respirometry: O2k-Open Support 

» TPP: O2k-technical_support mt-membrane potential
» Calcium: O2k-technical_support
» Other ions beside TPP+, TPMP+, and Ca2+: There are ion selective membranes available, e.g., from Sigma Aldrich, for a wide variety of ionic species. If cut to a circle with a diameter of 4 mm these membranes can be used in the ISE system. Many ions may also be detected by fluorimetric based methods.

Keywords: Force and membrane potential


Questions.jpg


Click to expand or collaps
» Keywords: Force and membrane potential
Fundamental relationships
» Force
» Affinity
» Flux
» Advancement
» Advancement per volume
» Stoichiometric number
mt-Membrane potential and protonmotive force
» Protonmotive force
» Mitochondrial membrane potential
» Chemical potential
» Faraday constant
» Format
» Uncoupler
O2k-Potentiometry
» O2k-Catalogue: O2k-TPP+ ISE-Module
» O2k-Manual: MiPNet15.03 O2k-MultiSensor-ISE
» TPP - O2k-Procedures: Tetraphenylphosphonium
» Specifications: MiPNet15.08 TPP electrode
» Poster
» Unspecific binding of TPP+
» TPP+ inhibitory effect
O2k-Fluorometry
» O2k-Catalogue: O2k-FluoRespirometer
» O2k-Manual: MiPNet22.11 O2k-FluoRespirometer manual
» Safranin - O2k-Procedures: MiPNet20.13 Safranin mt-membranepotential / Safranin
» TMRM - O2k-Procedures: TMRM
O2k-Publications
» O2k-Publications: mt-Membrane potential
» O2k-Publications: Coupling efficiency;uncoupling



Labels: MiParea: Instruments;methods 





HRR: Oxygraph-2k, TPP, O2k-Manual 

O2k-MultiSensor, DatLab, DL6, DL6a7