MiPNet11.05 imt-pti-pce: Difference between revisions
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Whereas isolated mitochondria remain one of the gold-standards in studies of bioenergetics and mitochondrial physiology, permeabilized tissues and cells have become an alternative with several advantages. But some disadvantages have to be considered, too, for optimum experimental design and critical evaluation of results. | Whereas isolated mitochondria remain one of the gold-standards in studies of bioenergetics and mitochondrial physiology, permeabilized tissues and cells have become an alternative with several advantages. But some disadvantages have to be considered, too, for optimum experimental design and critical evaluation of results. | ||
:>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents] | :>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents] | ||
:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS | :>> Product: [http://www.oroboros.at/?oxygraph OROBOROS O2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]] | ||
|mipnetlab=AT_Innsbruck_OROBOROS | |mipnetlab=AT_Innsbruck_OROBOROS | ||
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Revision as of 11:45, 13 May 2015
Isolated mitochondria or permeabilized tissues and cells. |
ยป Open Access.MiPNet11.05.pdf, Versions
OROBOROS (2014-04-06) Mitochondr Physiol Network
Abstract: Gnaiger E (2014) Isolated mitochondria or permeabilized tissues and cells. Mitochondr Physiol Network 11.05(06): 1-5.
Whereas isolated mitochondria remain one of the gold-standards in studies of bioenergetics and mitochondrial physiology, permeabilized tissues and cells have become an alternative with several advantages. But some disadvantages have to be considered, too, for optimum experimental design and critical evaluation of results.
- >> O2k-Protocols:Overall contents
- >> Product: OROBOROS O2k, O2k-Catalogue
โข O2k-Network Lab: AT_Innsbruck_OROBOROS
Labels: MiParea: mt-Biogenesis;mt-density
Preparation: Permeabilized cells, Permeabilized tissue, Isolated mitochondria
HRR: Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
Mt-Preparation
Further information
We recommend the new METTLER TOLEDO microbalances XSE105DU for determination of tissue wet weight (1 to 3 mg wet weight per chamber). We offer this microbalance as part of our systems-approach to high-resolution respirometry.