MiPNet11.05 imt-pti-pce: Difference between revisions
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|year=2005 | |year=2005 | ||
|journal=Mitochondr Physiol Network | |journal=Mitochondr Physiol Network | ||
|abstract= | |abstract='''O2k-Protocol for Isolated mitochondria or permeabilized tissues and cells''' | ||
'''O2k-Protocol for Isolated mitochondria or permeabilized tissues and cells''' | |||
Summary: Whereas isolated mitochondria remain one of the gold-standards in studies of bioenergetics and mitochondrial physiology, permeabilized tissues and cells have become an alternative with several advantages. But some disadvantages have to be considered, too, for optimum experimental design and critical evaluation of results. | Summary: Whereas isolated mitochondria remain one of the gold-standards in studies of bioenergetics and mitochondrial physiology, permeabilized tissues and cells have become an alternative with several advantages. But some disadvantages have to be considered, too, for optimum experimental design and critical evaluation of results. | ||
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|instruments=Protocol | |instruments=Protocol | ||
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==Further information == | |||
We recommend the new [http://at.mt.com/at/de/home/products/Laboratory_Weighing_Solutions/Analytical_Balances/Excellence_Analytical.html?com.broadvision.session.new=Yes METTLER TOLEDO] microbalances XS105DU or XS205DU for determination of tissue wet weight (1 to 3 mg wet weight per chamber).ย We offer these microbalances as part of our systems-approach to high-resolution respirometry. | |||
:>> [http://www.oroboros.at/?Mitochondria-Tissue www Tisues] | :>> [http://www.oroboros.at/?Mitochondria-Tissue www Tisues] | ||
Revision as of 13:51, 29 November 2013
Gnaiger E (2005) Isolated mitochondria or permeabilized tissues and cells. Mitochondr Physiol Network 11.05. |
ยป Open Access.MiPNet11.05.pdf, Versions
OROBOROS (2005) Mitochondr Physiol Network
Abstract: O2k-Protocol for Isolated mitochondria or permeabilized tissues and cells
Summary: Whereas isolated mitochondria remain one of the gold-standards in studies of bioenergetics and mitochondrial physiology, permeabilized tissues and cells have become an alternative with several advantages. But some disadvantages have to be considered, too, for optimum experimental design and critical evaluation of results.
- >> O2k-Protocols:Overall contents
- >> Product: OROBOROS Oxygraph-2k, O2k-Catalogue
โข O2k-Network Lab: AT_Innsbruck_OROBOROS
Labels: MiParea: mt-Biogenesis;mt-density
Preparation: Permeabilized cells, Permeabilized tissue, Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
HRR: Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
Further information
We recommend the new METTLER TOLEDO microbalances XS105DU or XS205DU for determination of tissue wet weight (1 to 3 mg wet weight per chamber). We offer these microbalances as part of our systems-approach to high-resolution respirometry.
- >> www Tisues
- Version 5: 2011-12-03
- Version 1: 2005