MiPNet09.12 O2k-Titrations
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O2k manual titrations: SUIT protocols with mitochondrial preparations. |
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Oroboros (2019-10-23) Mitochondr Physiol Network
Abstract: Oroboros (2019) O2k manual titrations: SUIT protocols with mitochondrial preparations. Mitochondr Physiol Network 09.12(19): 1-4.
A list of substrates, uncouplers, and inhibitors used in SUIT protocols in high-resolution respirometry, with information on abbreviations, stock concentrations, final experimental concentrations, volumes titrated into the 2 mL O2k-chamber and 0.5 mL O2k-Chamber sV (O2k-sV-Module), and syringes used for manual titrations.
- Β» O2k-Protocols
- Β» Product: Oroboros O2k, O2k-Catalogue
β’ O2k-Network Lab: AT_Innsbruck_Oroboros
Labels: MiParea: Instruments;methods
Regulation: ADP, Inhibitor, Substrate, Uncoupler, Amino acid
HRR: O2k-Protocol
O2k-chemicals and media
Note: Titration of chemicals in respirometry
- Overshoots or short interruptions of the flux after the injection of chemicals do not have a meaning in respirometric measurements. A disturbance of the signal is only observed due to the injected chemical and does not represent mitochondrial respiration. With the addition of a chemical solved in ethanol a little bit of oxygen is always introduced, since ethanol has higher oxygen solubility than water.
Further information
- Β» MitoPedia: SUIT - Substrate-uncoupler-inhibitor-titration (SUIT) protocols for respirometry
- Β» MiPNet03.02 - Chemicals and media for respirometry
- Β» MiPNet19.14 - SOP for manual O2k-titrations with Hamilton microsyringes.