MiPNet08.12 IOC22: Difference between revisions
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== Further information == | == Further information == | ||
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* Version 7: 2011-12-11 | * Version 7: 2011-12-11 |
Revision as of 15:08, 29 November 2013
Gnaiger E, Doeller JE, Kraus D, Shiva S, Brookes PS, Darley-Usmar VM (2003) NO effect on mitochondrial oxygen kinetics at low oxygen. Oxygraph-2k workshop Report. Mitochondr Physiol Network 08.12. |
ยป Open Access-MiPNet08.12.pdf; Versions
OROBOROS (2003) Mitochondr Physiol Network
Abstract: O2k-Protocol for NO effect on mitochondrial oxygen kinetics at low oxygen
A single pilot experiment was carried out during an Oxygraph-2k workshop on high-resolution respirometry (IOC22). Respiration of isolated rat liver mitochondria was inhibited by addition of NO, which increased the sensitivity to oxygen >25-fold when compared to the half-saturation oxygen pressure, p50, in the absence of NO. Oxygen kinetics followed a monophasic hyperbolic function up to 2.2 kPa with NO (p50=0.93 kPa), compared to the standard oxygen range to 1.1 kPa without NO (p50=0.035 kPa).
Reference: Biochim Biophys Acta 1797: 557-565 (2010)
See also: Combined O2 and NO measurement and oxygen kinetics
- >> O2k-Protocols:Overall contents
- >> Product: OROBOROS Oxygraph-2k, O2k-Catalogue
โข O2k-Network Lab: AT_Innsbruck_OROBOROS, US_AL Birmingham_Kraus DW
Labels: MiParea: Respiration, Instruments;methods
Stress:Hypoxia, RONS; Oxidative Stress"RONS; Oxidative Stress" is not in the list (Cell death, Cryopreservation, Ischemia-reperfusion, Permeability transition, Oxidative stress;RONS, Temperature, Hypoxia, Mitochondrial disease) of allowed values for the "Stress" property. Organism: Rat Tissue;cell: Liver Preparation: Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Regulation: Inhibitor, O2"O2" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property. Coupling state: OXPHOS
HRR: Oxygraph-2k, NO, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
O2k-Demo
Further information
- Version 7: 2011-12-11
- Version 1: 2003