MiPNet08.12 IOC22: Difference between revisions
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{{Publication | {{Publication | ||
|title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] NO effect on mitochondrial oxygen kinetics at low oxygen. Oxygraph-2k workshop Report. | |title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] NO effect on mitochondrial oxygen kinetics at low oxygen. Oxygraph-2k workshop Report. | ||
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|organism=Rat | |organism=Rat | ||
|tissues=Liver | |tissues=Liver | ||
|preparations=Isolated | |preparations=Isolated mitochondria | ||
|injuries=Hypoxia, RONS | |injuries=Hypoxia, Oxidative stress;RONS | ||
|topics=Inhibitor, O2 | |topics=Inhibitor, O2 | ||
|couplingstates=OXPHOS | |couplingstates=OXPHOS | ||
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|additional=O2k-Demo, O2k-MultiSensor | |additional=O2k-Demo, O2k-MultiSensor | ||
}} | }} | ||
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Revision as of 14:31, 13 February 2015
NO effect on mitochondrial oxygen kinetics at low oxygen. Oxygraph-2k workshop Report. |
ยป Open Access-MiPNet08.12.pdf; Versions
OROBOROS (2011-12) Mitochondr Physiol Network
Abstract: Gnaiger E, Doeller JE, Kraus D, Shiva S, Brookes PS, Darley-Usmar VM (2011) NO effect on mitochondrial oxygen kinetics at low oxygen. Oxygraph-2k workshop Report. Mitochondr Physiol Network 08.12(07).
A single pilot experiment was carried out during an Oxygraph-2k workshop on high-resolution respirometry (IOC22). Respiration of isolated rat liver mitochondria was inhibited by addition of NO, which increased the sensitivity to oxygen >25-fold when compared to the half-saturation oxygen pressure, p50, in the absence of NO. Oxygen kinetics followed a monophasic hyperbolic function up to 2.2 kPa with NO (p50=0.93 kPa), compared to the standard oxygen range to 1.1 kPa without NO (p50=0.035 kPa).
Reference: Biochim Biophys Acta 1797: 557-565 (2010)
See also: Combined O2 and NO measurement and oxygen kinetics
- >> Product: OROBOROS Oxygraph-2k, O2k-Catalogue
โข O2k-Network Lab: AT_Innsbruck_OROBOROS, US_AL Birmingham_Kraus DW
Labels: MiParea: Respiration, Instruments;methods
Stress:Hypoxia, Oxidative stress;RONS Organism: Rat Tissue;cell: Liver Preparation: Isolated mitochondria
Regulation: Inhibitor, O2"O2" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property. Coupling state: OXPHOS
HRR: Oxygraph-2k, NO, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
O2k-Demo, O2k-MultiSensor