MiPMap: Difference between revisions
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:: * Preparation of isolated mitochondria, permeabilized tissuem, homogenate. | :: * Preparation of isolated mitochondria, permeabilized tissuem, homogenate. | ||
:: * Which respiration medium is recommended? ->> Do not use media with K<sup>+</sup>, which inhibits kidney mitochondrial respiration. | :: * Which respiration medium is recommended? ->> Do not use media with K<sup>+</sup>, which inhibits kidney mitochondrial respiration. | ||
>> Is this an organ OR species issue? (according to [[Calzia E]] MiR05 works fine with kidney tissue from pig, but not in mouse) | |||
:: * Automatically generated list of references: | :: * Automatically generated list of references: | ||
Revision as of 16:59, 24 January 2013
Description
Mitochondrial Physiology Map - MiPMap
The project Mitochondrial Physiology Map is initiated to create a list of mitochondrial properties in cell types, tissues and species. As part of MitoPedia, MiPMap will provide an information synthase for Comparative Mitochondrial Physiology. This project will require global input to establish a comprehensive database.
Work in progress
MiPMap List of Mitochondrial Properties in Cell Types, Tissues and Species
1. Species
- For example, in this list you may find information on mouse cell lines and tissues, e.g. mouse kidney mitochondria:
- * Is malic enzyme present?
- * Is the P/E<1.0 like in mouse brain but different from mouse skeletal muscle?
- * Which UCPs are present?
- * Preparation of isolated mitochondria, permeabilized tissuem, homogenate.
- * Which respiration medium is recommended? ->> Do not use media with K+, which inhibits kidney mitochondrial respiration.
>> Is this an organ OR species issue? (according to Calzia E MiR05 works fine with kidney tissue from pig, but not in mouse)
- * Automatically generated list of references:
2. Cell types and Tissues
- Or you may find Skeletal muscle mitochondria (or split into aerobic/glycolytic, ..) studied across species:
- * Human - mitochondrial preparations (isolated mitochondria, permeabilized fibres, homogenate)
- * Human populations
- * Mouse / Mouse strains
- * Rat
- * Pig
- * Equine
- * Fish, Squid, ...
3. Organisms
Work in progress
4. Diseases
Disease models:
Cardiovascular
Heart failure,
Respiratory diseases
COPD,
Metabolic diseases
Diabetes,
Neurodegenerative diseases
Parkinson's disease, Alzheimer's disease
Initiation
- Presented at Bioblast 2012.
- Focus for MiPconference on Comparative Mitochondrial Physiology.
References and notes