Glucose: Difference between revisions

Revision as of 13:47, 18 May 2017

high-resolution terminology - matching measurements at high-resolution

Glucose

Description

Glucose, also known as D-glucose or dextrose, is a monosaccharide and an important carbohydrate in biology. Cells use it as the primary source of energy and a metabolic intermediate.

Abbreviation: Glc

Reference: MiPNet15.09. Yeast: HRR Reference Assay

MitoPedia topics: Substrate and metabolite

Application in HRR

Glc: Glucose (D-(+)-Glucose, C₆H₁₂0₆); Sigma G 8270, 100 g, store at R.T.; FW = 180.16

Preparation of 2 M stock solution (dissolved in H₂O):

1) weigh 360.32 mg of Glucose

2) dissolve 1 ml H₂O in 1,5 ml Eppendorf

Oxygraph-2k Titration:

Titration volume: 20 µl using a 50 µl syringe
Final conc. in 2 ml O2k-chamber: 20 mM

Preparation of 2.2 M stock solution (dissolved in H₂O):

1) weigh 79.2 mg of Glucose

2) dissolve 0.2 ml H₂O in 0,5 ml Eppendorf

Oxygraph-2k Titration:

Titration volume: 5 µl using a 25 µl syringe
Final conc. in 2 ml O2k-chamber: 5.5 mM (1 g/L)
Titration volume: 10 µl using a 25 µl syringe
Final conc. in 2 ml O2k-chamber: 11 mM (2 g/L)

@@ Line 18: / Line 18: @@
 ::1) weigh 360.32 mg of Glucose
-::2) dissolve 1 ml H<sub>2</sub>O in 1,5ml Eppendorf
+::2) dissolve 1 ml H<sub>2</sub>O in 1,5 ml Eppendorf
 '''Oxygraph-2k Titration:'''
@@ Line 24: / Line 24: @@
 ::* Titration volume: 20 µl using a 50 µl syringe
 ::* Final conc. in 2 ml O2k-chamber: 20 mM
+'''Preparation of 2.2 M stock solution''' (dissolved in H<sub>2</sub>O):
+::1) weigh 79.2 mg of Glucose
+::2) dissolve 0.2 ml H<sub>2</sub>O in 0,5 ml Eppendorf
+'''Oxygraph-2k Titration:'''
+::* Titration volume: 5 µl using a 25 µl syringe
+::* Final conc. in 2 ml O2k-chamber: 5.5 mM (1 g/L)
+::* Titration volume: 10 µl using a 25 µl syringe
+::* Final conc. in 2 ml O2k-chamber: 11 mM (2 g/L)