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Doerrier 2020 PaduaMuscleDays Poster

From Bioblast
Doerrier C, Gama-Perez P, Chabi B, Ost M, Distefano G, Pesta D, Dahdah N, Lemieux H, Holody C, Carpenter RG, Tepp K, Puurand M, Kaambre T, Dubouchaud H, Cortade F, Calabria E, Casado M, Fernandez-Ortiz M, Acuna-Castroviejo D, Villena JA, Grefte S, Keijer J, O'Brien K, Sowton A, Murray AJ, Campbell MD, Marcinek DJ, Nollet E, Wuest R, Dayanidhi S, Soendergaard SD, Chroeis KM, Gonzalez-Franquesa A, Goodpaster BH, Coen PM, Larsen S, Gnaiger E, Garcia-Roves PM (2020) Commitment to reproducibility in mitochondrial respiration studies with permeabilized muscle fibers. PaduaMuscleDays.

Link: Padova Muscle Days

Doerrier C, Gama-Perez P, Chabi B, Ost M, Distefano G, Pesta D, Dahdah N, Lemieux H, Holody C, Carpenter RG, Tepp K, Puurand M, Kaambre T, Dubouchaud H, Cortade F, Calabria E, Casado M, Fernandez-Ortiz M, Acuna-Castroviejo D, Villena JA, Grefte S, Keijer J, O'Brien K, Sowton A, Murray AJ, Campbell MD, Marcinek DJ, Nollet E, Wuest R, Dayanidhi S, Soendergaard SD, Chroeis KM, Gonzalez-Franquesa A, Goodpaster BH, Coen PM, Larsen S, Gnaiger E, Garcia-Roves PM (2020)

Event: 2020 PaduaMuscleDays Padova IT

The exponential increase of scientific publications in the mitochondrial field shows the growing interest in mitochondria. However, the lack of methodological consistency in many published projects on mitochondrial respiratory function complicates a quantitative inter/intra-laboratory comparison of datasets. This deficiency manifests the need to improve the quality in science[1]. In this context, the MitoEAGLE COST Action is a powerful framework committed to evaluate and enhance the reproducibility in mitochondrial physiology as a basis to establish a novel mitochondrial database related to Evolution, Age, Gender, Lifestyle and Environment. Permeabilized muscle fibers are widely used to evaluate mitochondrial function in health and disease[2]. Therefore, our main goals are to: 1) compare protocols used in different research laboratories, 2) analyze factors which contribute to experimental variability, 3) define optimal experimental conditions in muscle studies, 4) elaborate guidelines for evaluating mitochondrial function in muscle tissue, 5) establish reference values on mitochondrial respiration, particularly as a test of the skills in preparing high-quality permeabilized muscle fibers, and 6) generate a database. To achieve our aims, two unique studies are currently in progress: 1) 17 international research groups performing independently experiments on respiration in permeabilized fibers of mouse soleus muscle, following the same experimental procedure[3]; 2) a blinded international study measuring simultaneously in the same laboratory respiration of permeabilized human skeletal fibers by high-resolution respirometry and assessing the effect of different experimental conditions[4]. Our results contribute to face the reproducibility crisis and provide the basis for establishing the first database on mitochondrial respiratory parameters in muscle tissues.

Keywords: Mitochondria, mitochondrial database, mitochondrial respiration, reproducibility crisis, permeabilized muscle fibers, reference values Bioblast editor: Plangger M O2k-Network Lab: AT Innsbruck Oroboros, FR Montpellier Wrutniak-Cabello C, DE Nuthetal Klaus S, US FL Orlando Goodpaster BH, DE Duesseldorf Roden M, ES Barcelona Garcia-Roves PM, CA Edmonton Lemieux H, AT Innsbruck Gnaiger E, AT Innsbruck MitoFit, EE Tallinn Kaambre T, IT Verona Calabria E, ES Valencia Casado Pinna M, ES Granada Acuna-Castroviejo D, NL Nijmegen Koopman WJH, NL Wageningen Keijer J, US AK Fairbanks O'Brien K, UK Cambridge Murray AJ, US WA Seattle Marcinek DJ, NL Amsterdam Wuest RC, US IL Chicago Dayanidhi S, DK Copenhagen Larsen S


Labels: MiParea: Respiration 


Organism: Human, Mouse  Tissue;cell: Skeletal muscle  Preparation: Permeabilized tissue 


Coupling state: LEAK, OXPHOS, ET  Pathway: N, S, NS, ROX  HRR: Oxygraph-2k  Event: Poster  MitoEAGLE 

Affiliations and support

Carolina Doerrier (1), Pau Gama-Perez (2), Beatrice Chabi (3), Mario Ost (4), Giovanna Distefano (5), Dominik Pesta (6,7), Norma Dahdah (2), Hélène Lemieux (8), Claudia Holody (8), Rowan G Carpenter (8), Kersti Tepp (9), Marju Puurand (9), Tuuli Kaambre (9), Hervé Dubouchaud (10), Fabienne Cortade (3), Elisa Calabria (11), Marta Casado (12), Marisol Fernandez-Ortiz (13), Dario Acuna-Castroviejo (13), Josep A Villena (14), Sander Grefte (15), Jaap Keijer (15), Kristin O'Brien (16), Alice Sowton (16), Andrew James Murray (16), Matthew D Campbell (17), David J Marcinek (17), Edgar Nollet (18), Rob Wuest (18), Sudarshan Dayanidhi (19), Stine Dam Soendergaard (20), Karoline Maise Chroeis (20), Alba Gonzalez-Franquesa (21), Bret H Goodpaster (5), Paul M Coen (5), Steen Larsen (20), Erich Gnaiger (1,22), Pablo Miguel Garcia-Roves (2)
  1. Oroboros Instruments, Innsbruck, Austria
  2. Dept Physiological Sciences, Univ Barcelona Bellvitge Biomedical Research Inst (IDIBELL), Spain
  3. DMEM, INRA, Univ Montpellier, France
  4. German Inst Human Nutrition Potsdam-Rehbruecke, Potsdam, Germany
  5. Translational Research Inst Metabolism Diabetes, Florida Hospital, Orlando, FL, USA
  6. Inst Clinical Diabetology, German Diabetes Center, Leibniz Center Diabetes Research Heinrich-Heine Univ Düsseldorf
  7. German Center Diabetes Research, Munich, Neuherberg; Germany
  8. Fac Saint-Jean, Univ Alberta, Canada
  9. Lab Chemical Biology, National Inst Chemical Physics Biophysics, Tallinn, Estonia
  10. Lab Bioénergétique Fondamentale Appliquée, Univ Grenoble Alpes, INSERM, Grenoble, France
  11. Dept Neurological Movement Sciences, Univ Verona, Italy
  12. Dept Molecular Cellular Pathology Therapy, Inst Biomedicina de Valencia, Spain
  13. Biomedical Research Center, Univ Granada, Spain
  14. Metabolism Obesity Lab, Vall d’Hebron Research Inst, Barcelona, Spain
  15. Human Animal Physiology, Wageningen Univ, The Netherlands
  16. Dept Physiology, Development Neuroscience, Univ Cambridge, UK
  17. Dept Radiology, Univ Washington, South Lake Union, USA
  18. Dept Human Movement Sciences, Fac Behavioural Movement Sciences, Vrije Univ Amsterdam, The Netherlands
  19. Rehabilitation Inst Chicago, Feinberg School Medicine, Northwestern Univ, USA
  20. Dept Biomedical Sciences, Center Healthy Aging, Fac Health Sciences, Denmark
  21. The Novo Nordisk Center Basic Metabolic Research, Section Integrative Physiology; Univ Copenhagen, Denmark
  22. D Swarovski Research Lab, Dept Visceral, Transplant Thoracic Surgery, Med Univ Innsbruck, Austria. - [email protected]
Support: MitoEAGLE Task Group WG2. Contribution to European Union Framework Programme Horizon 2020 COST Action CA15203 MitoEAGLE.

References

  1. Baker M (2016) 1,500 scientists lift the lid on reproducibility. Survey sheds light on the ‘crisis’ rocking research. Nature 533:452–4.
  2. Gnaiger E (2009) Capacity of oxidative phosphorylation in human skeletal muscle. New perspectives of mitochondrial physiology. Int J Biochem Cell Biol 41:1837-45.
  3. Chabi B, Ost M, Gama-Perez P, Dahdah N, Lemieux H, Holody CD, Carpenter RG, Tepp K, Puurand M, Kaambre T, Dubouchaud H, Cortade F, Pesta D, Calabria E, Casado M, Fernandez-Ortiz M, Acuña-Castroviejo D, Villena JA, Grefte S, Keijer J, O'Brien K, Sowton A, Murray AJ, Campbell MD, Marcinek DJ, Nollet E, Wüst R, Dayanidhi S, Gnaiger E, Doerrier C, Garcia-Roves PM (2019) Generating reference values on mitochondrial respiration in permeabilized muscle fibers. MitoFit Preprint Arch. doi:10.26124/mitofit:ea19.MitoEAGLE.0001.
  4. Doerrier C, Gama-Perez P, Distefano G, Pesta D, Soendergaard SD, Chroeis KM, Gonzalez-Franquesa A, Goodpaster BH, Coen P, Larsen S, Gnaiger E, Garcia-Roves PM (2019) Inter-laboratory harmonization of respiratory protocols in permeabilized human muscle fibers. MitoFit Preprint Arch. doi:10.26124/mitofit:ea19. MiPSchool .0009.