Baris 2015 Am J Physiol Regul Integr Comp Physiol: Difference between revisions
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{{Publication | {{Publication | ||
|title=Baris TZ, Crawford DL, Oleksiak MF (2015) Acclimation and acute temperature effects on population differences in oxidative phosphorylation. Am J Physiol Regul Integr Comp Physiol | |title=Baris TZ, Crawford DL, Oleksiak MF (2015) Acclimation and acute temperature effects on population differences in oxidative phosphorylation. Am J Physiol Regul Integr Comp Physiol 310:R185-96. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/26582639 PMID: 26582639] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/26582639 PMID: 26582639] | ||
|authors=Baris TZ, Crawford DL, Oleksiak MF | |authors=Baris TZ, Crawford DL, Oleksiak MF | ||
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{{Labeling | {{Labeling | ||
|area=Respiration, Comparative MiP;environmental MiP | |area=Respiration, Comparative MiP;environmental MiP | ||
| | |injuries=Temperature | ||
|organism=Fishes | |||
|tissues=Heart | |tissues=Heart | ||
| | |preparations=Permeabilized tissue | ||
|couplingstates=LEAK, OXPHOS, | |couplingstates=LEAK, OXPHOS, ET | ||
| | |pathways=N, S, CIV, NS, Other combinations, ROX | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional= | |additional=2015-12 | ||
}} | }} |
Latest revision as of 16:52, 9 November 2017
Baris TZ, Crawford DL, Oleksiak MF (2015) Acclimation and acute temperature effects on population differences in oxidative phosphorylation. Am J Physiol Regul Integr Comp Physiol 310:R185-96. |
Baris TZ, Crawford DL, Oleksiak MF (2015) Am J Physiol Regul Integr Comp Physiol
Abstract: Temperature changes affect metabolism on acute, acclimatory and evolutionary time scales. To better understand temperature's affect on metabolism at these different time scales, we quantified cardiac oxidative phosphorylation (OxPhos) in three Fundulus taxa acclimated to 12ยฐC and 28ยฐC and measured at three acute temperatures (12ยฐC, 20ยฐC, and 28ยฐC). The Fundulus taxa (northern Maine and southern Georgia F. heteroclitus, and a sister taxa, F. grandis) were used to identify evolved changes in OxPhos. Cardiac OxPhos metabolism was quantified by measuring six traits: State 3 (ADP and substrate dependent mitochondrial respiration), E State (uncoupled mitochondrial activity), Complex I, II, and IV activities, and LEAK ratio. Acute temperature affected all OxPhos traits. Acclimation only significantly affected State 3 and LEAK ratio. Populations were significantly different for State 3. In addition to direct effects, there were significant interactions between acclimation and population for Complex I and between population and acute temperature for State 3. Further analyses suggest that acclimation alters the acute temperature response for State 3, E State, and Complexes I and II: at the low acclimation temperature, the acute response was dampened at low assay temperatures, and at the high acclimation temperature, the acute response was dampened at high assay temperatures. Closer examination of the data also suggests that differences in State 3 respiration and Complex I activity between populations were greatest between fish acclimated to low temperatures when assayed at high temperatures, suggesting that differences between the populations become more apparent at the edges of their thermal range. โข Keywords: Fundulus, State 3, Enzyme complexes, Mitochondria
โข O2k-Network Lab: US FL Miami Crawford DL
Labels: MiParea: Respiration, Comparative MiP;environmental MiP
Stress:Temperature Organism: Fishes Tissue;cell: Heart Preparation: Permeabilized tissue
Coupling state: LEAK, OXPHOS, ET
Pathway: N, S, CIV, NS, Other combinations, ROX
HRR: Oxygraph-2k
2015-12