Difference between revisions of "Atractyloside"
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{{MitoPedia | {{MitoPedia | ||
|abbr=Atr | |abbr=Atr | ||
|description='''Atractyloside''' is an inhibitor of the [[adenine nucleotide translocator|Adenine nucleotide translocator (ANT)]]. | |description='''Atractyloside''' is an inhibitor of the [[adenine nucleotide translocator|Adenine nucleotide translocator (ANT)]]. It is an extremely toxic glycoside that inhibits oxidative phosphorylation by blocking the transfer of adenosine nucleotides through the mitochondrial membrane. | ||
|info=[[MiPNet03.02]]; [[MiPNet09.12]] | |info=[[MiPNet03.02]]; [[MiPNet09.12]] | ||
|type=Inhibitor PS | |type=Inhibitor PS | ||
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|type=Inhibitor PS | |type=Inhibitor PS | ||
}} | }} | ||
With Atr, a very high concentration is required for full inhibition. [[carboxyatractyloside|Carboxyatractyloside]] (Cat) is considered to be much more specific than Atr, and the effective concentration required is much much lower. | |||
== Application in [[HRR]] == | |||
'''Atr: Atractyloside''' (Atractyloside dipotassium salt, C<sub>30</sub>H<sub>44</sub>O<sub>16</sub>S<sub>2</sub>K<sub>2</sub>); Sigma A 6882, 250 mg, store at R.T.: FW = 802.99 | |||
'''Preparation of 50 mM stock solution''' (dissolved in H20): | |||
::1) weigh 40.2 mg of Atractyloside | |||
::2) dissolve in 1 ml H<sub>2</sub>O (it dissolves better in warm water!) | |||
::3) divide into small aliquots | |||
::4) store frozen at -20 °C | |||
'''Oxygraph-2k Manual Titrations:''' [[MiPNet09.12]] | |||
::* Titration volume: 30 µl using a 50 µl syringe | |||
::* Final conc. in 2 ml O2k-chamber: 0.75 mM | |||
'''Comment:''' With Atr, a very high concentration is required for full inhibition. [[carboxyatractyloside|Carboxyatractyloside]] (Cat) is considered to be much more specific than Atr, and the effective concentration required is much much lower. |
Revision as of 08:30, 18 May 2012
Description
Atractyloside is an inhibitor of the Adenine nucleotide translocator (ANT). It is an extremely toxic glycoside that inhibits oxidative phosphorylation by blocking the transfer of adenosine nucleotides through the mitochondrial membrane.
Abbreviation: Atr
Reference: MiPNet03.02; MiPNet09.12
MitoPedia topics: Inhibitor
Application in HRR
Atr: Atractyloside (Atractyloside dipotassium salt, C30H44O16S2K2); Sigma A 6882, 250 mg, store at R.T.: FW = 802.99
Preparation of 50 mM stock solution (dissolved in H20):
- 1) weigh 40.2 mg of Atractyloside
- 2) dissolve in 1 ml H2O (it dissolves better in warm water!)
- 3) divide into small aliquots
- 4) store frozen at -20 °C
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 30 µl using a 50 µl syringe
- Final conc. in 2 ml O2k-chamber: 0.75 mM
Comment: With Atr, a very high concentration is required for full inhibition. Carboxyatractyloside (Cat) is considered to be much more specific than Atr, and the effective concentration required is much much lower.