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Difference between revisions of "Appaix 2002 Biochim Biophys Acta"

From Bioblast
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|organism=Rat
|organism=Rat
|tissues=Cardiac muscle
|tissues=Cardiac muscle
|preparations=Permeabilized Cells, Isolated Mitochondria
|preparations=Permeabilized cells, Isolated Mitochondria
|topics=Coupling; Membrane Potential, ATP; ADP; AMP; PCr
|topics=Coupling; Membrane Potential, ATP; ADP; AMP; PCr
|discipline=Mitochondrial Physiology
|discipline=Mitochondrial Physiology
}}
}}

Revision as of 07:28, 5 April 2012

Publications in the MiPMap
Appaix F, Guerrero K, Rampal D, Izikki M, Kaambre T, Sikk P, Brdiczka D, Riva-Lavieille C, Olivares J, Longuet M, Antonsson B, Saks VA (2002) Bax and heart mitochondria: uncoupling and inhibition of respiration without permeability transition. Biochim Biophys Acta 1556: 155-167.


Appaix F, Guerrero K, Rampal D, Izikki M, Kaambre T, Sikk P, Brdiczka D, Riva-Lavieille C, Olivares J, Longuet M, Antonsson B, Saks VA (2002) Biochim Biophys Acta

Abstract: The effects of Bax (full-length, FL, and C-terminal truncated, Ξ”C) on respiration rate, membrane potential, MgATPase activity and kinetics of regulation of respiration were studied in isolated rat heart mitochondria and permeabilized cardiomyocytes. The results showed that while both Bax-FL and Bax-Ξ”C permeabilized the outer mitochondrial membrane, released cytochrome c and reduced the respiration rate, the latter could be fully restored by exogenous cytochrome c only in the case of Bax-Ξ”C, but not in presence of Bax-FL. In addition, Bax-FL but not Bax-Ξ”C increased the MgATPase activity, and their effects on the mitochondrial membrane potential were quantitatively different. None of these effects was sensitive to cyclosporin A (CsA).

It is concluded that Bax-FL affects both the outer and the inner mitochondrial membranes by: (1) opening large pores in the outer membrane; (2) inhibiting some segments of the respiratory chain in the inner membrane; and (3) uncoupling the inner mitochondrial membrane by increasing proton leak without opening the permeability transition pore (PTP). β€’ Keywords: Mitochondrion, Apoptosis, Heart, Oxidative phosphorylation, Bax

β€’ O2k-Network Lab: DE_Konstanz_Brdiczka D, EE_Tallinn_Saks VA, FR_Grenoble_Saks VA


Labels:


Organism: Rat  Tissue;cell: Cardiac muscle"Cardiac muscle" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property.  Preparation: Permeabilized cells, Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property. 

Regulation: Coupling; Membrane Potential"Coupling; Membrane Potential" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., ATP; ADP; AMP; PCr"ATP; ADP; AMP; PCr" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property. 


HRR: Oxygraph-2k