Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Difference between revisions of "Appaix 2002 Biochim Biophys Acta"

From Bioblast
Line 15: Line 15:
|organism=Rat
|organism=Rat
|tissues=Cardiac Muscle
|tissues=Cardiac Muscle
|preparations=Isolated Mitochondria
|preparations=Isolated Mitochondria, Permeabilized Cell or Tissue; Homogenate
|enzymes=Inner mtMembrane Transporter
|topics=Coupling; Membrane Potential, ATP; ADP; AMP; PCr
|topics=Respiration; OXPHOS; ETS Capacity, Coupling; Membrane Potential, Ion Homeostasis
|discipline=Mitochondrial Physiology
|discipline=Mitochondrial Physiology
}}
}}

Revision as of 08:52, 8 September 2011

Publications in the MiPMap
Appaix F, Guerrero K, Rampal D, Izikki M, Kaambre T, Sikk P, Brdiczka D, Riva-Lavieille C, Olivares J, Longuet M, Antonsson B, Saks VA (2002) Bax and heart mitochondria: uncoupling and inhibition of respiration without permeability transition. Biochim. Biophys. Acta 1556: 155-167.


Appaix F, Guerrero K, Rampal D, Izikki M, Kaambre T, Sikk P, Brdiczka D, Riva-Lavieille C, Olivares J, Longuet M, Antonsson B, Saks VA (2002) Biochim. Biophys. Acta

Abstract: The effects of Bax (full-length, FL, and C-terminal truncated, Ξ”C) on respiration rate, membrane potential, MgATPase activity and kinetics of regulation of respiration were studied in isolated rat heart mitochondria and permeabilized cardiomyocytes. The results showed that while both Bax-FL and Bax-Ξ”C permeabilized the outer mitochondrial membrane, released cytochrome c and reduced the respiration rate, the latter could be fully restored by exogenous cytochrome c only in the case of Bax-Ξ”C, but not in presence of Bax-FL. In addition, Bax-FL but not Bax-Ξ”C increased the MgATPase activity, and their effects on the mitochondrial membrane potential were quantitatively different. None of these effects was sensitive to cyclosporin A (CsA).

It is concluded that Bax-FL affects both the outer and the inner mitochondrial membranes by: (1) opening large pores in the outer membrane; (2) inhibiting some segments of the respiratory chain in the inner membrane; and (3) uncoupling the inner mitochondrial membrane by increasing proton leak without opening the permeability transition pore (PTP). β€’ Keywords: Mitochondrion, Apoptosis, Heart, Oxidative phosphorylation, Bax

β€’ O2k-Network Lab: DE_Konstanz_Brdiczka D, EE_Tallinn_Saks V, FR_Grenoble_Saks V


Labels:


Organism: Rat  Tissue;cell: Cardiac Muscle"Cardiac Muscle" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property.  Preparation: Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property., Permeabilized Cell or Tissue; Homogenate"Permeabilized Cell or Tissue; Homogenate" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property. 

Regulation: Coupling; Membrane Potential"Coupling; Membrane Potential" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., ATP; ADP; AMP; PCr"ATP; ADP; AMP; PCr" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property. 


HRR: Oxygraph-2k