Amytal: Difference between revisions
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== Application in [[HRR]] == | |||
:::'''Amy: Amytal'''ย (Amytal sodium salt,ย C<sub>11</sub>H<sub>17</sub>N<sub>2</sub>O<sub>3</sub>Na);ย Sigma A 4430, 50 g; M = 248.25 gยทmol<sup>-1</sup>. ''The product is withdrawn from Sigma but some residual amounts are still available.'' | |||
::::<span style="color:#8B008B"> '''Caution:''' Light sensitive and toxic!</span> | |||
::::'''Preparation of 200 mM stock solution''' (dissolved in ethanol abs.): | |||
::::# Weigh 49.65 mg of amytal. | |||
::::# Dissolve in 10 mL 50% ethanol. | |||
::::# Divide into 500 ยตL aliquots (use dark glass vials - light sensitive). | |||
::::# Store at -20 ยฐC. | |||
{{MitoPedia methods|type=Inhibitor ETS | {{MitoPedia methods|type=Inhibitor ETS | ||
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Latest revision as of 11:53, 22 June 2021
Description
Amytal sodium salt (synonym: amobarbital; 5-Ethyl-5-isoamylbarbituric acid) is a barbiturate drug and an inhibitor of Complex I.
Abbreviation: Amy
Application in HRR
- Amy: Amytal (Amytal sodium salt, C11H17N2O3Na); Sigma A 4430, 50 g; M = 248.25 gยทmol-1. The product is withdrawn from Sigma but some residual amounts are still available.
- Caution: Light sensitive and toxic!
- Preparation of 200 mM stock solution (dissolved in ethanol abs.):
- Weigh 49.65 mg of amytal.
- Dissolve in 10 mL 50% ethanol.
- Divide into 500 ยตL aliquots (use dark glass vials - light sensitive).
- Store at -20 ยฐC.
MitoPedia topics:
Inhibitor