Talk:Pyruvate: Difference between revisions
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::::*ย 10,000 times (2019-08-07) | ::::*ย 10,000 times (2019-08-07) | ||
::::*ย 5,000 times (2016-03-21) | ::::*ย 5,000 times (2016-03-21) | ||
== Application in [[HRR]] (updated instructions) == | |||
::: '''P: Pyruvate''' (pyruvic acid, sodium salt, C<sub>3</sub>H<sub>3</sub>O<sub>3</sub>Na); Sigma P 2256, 25 g, store at 4 ยฐC; M = 110.0 gยทmol<sup>-1</sup> | |||
:::: It is possible to weigh the powder beforehand in the Eppendorf-type tubes and store these tubes at 4 ยฐC, to be diluted only on the day of use. | |||
:::: 2021-02: The preparation instructions were updated to take the volume of the solute (P) into account. The concentrations prepared following the former instructions (see Discussion section) are sufficiently high for SUIT protocol titrations. | |||
:::: '''Preparation of 2 M stock solution''' (200 ยตL, dissolved in H<sub>2</sub>O)ย for use in '''2-mL O2k-chamber''': | |||
::::# Prepare fresh everyday. | |||
::::# Weigh 44 mg of pyruvic acid directly into a 0.5 mL Eppendorf tube. | |||
::::# Add 180 ยตL H<sub>2</sub>O. | |||
::::# Adjust pH to 7.0. | |||
:::ยป '''O2k manual titrations'''ย [[MiPNet09.12 O2k-Titrations]] | |||
::::* Titration volume ('''2-mL O2k-chamber'''): 5 ยตL using a 25 ยตL Hamilton syringe. | |||
::::* Final concentration: 5 mM. | |||
:::: '''Preparation of 2.5 M stock solution''' (200 ยตL, dissolved in H<sub>2</sub>O)ย for use in '''0.5-mL O2k-chamber''': | |||
::::# Prepare fresh everyday. | |||
::::# Weigh 55 mg of pyruvic acid directly into a 0.5 mL Eppendorf tube. | |||
::::# Add 175 ยตL H<sub>2</sub>O. | |||
::::# Adjust pH to 7.0. | |||
:::ยป '''O2k manual titrations'''ย [[MiPNet09.12 O2k-Titrations]] | |||
::::* Titration volume ('''0.5-mL O2k-chamber'''): 1 ยตL using a 10 ยตL Hamilton syringe. | |||
::::* Final concentration: 5 mM. |
Revision as of 12:36, 15 February 2021
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Application in HRR (updated instructions)
- P: Pyruvate (pyruvic acid, sodium salt, C3H3O3Na); Sigma P 2256, 25 g, store at 4 ยฐC; M = 110.0 gยทmol-1
- It is possible to weigh the powder beforehand in the Eppendorf-type tubes and store these tubes at 4 ยฐC, to be diluted only on the day of use.
- 2021-02: The preparation instructions were updated to take the volume of the solute (P) into account. The concentrations prepared following the former instructions (see Discussion section) are sufficiently high for SUIT protocol titrations.
- P: Pyruvate (pyruvic acid, sodium salt, C3H3O3Na); Sigma P 2256, 25 g, store at 4 ยฐC; M = 110.0 gยทmol-1
- Preparation of 2 M stock solution (200 ยตL, dissolved in H2O) for use in 2-mL O2k-chamber:
- Prepare fresh everyday.
- Weigh 44 mg of pyruvic acid directly into a 0.5 mL Eppendorf tube.
- Add 180 ยตL H2O.
- Adjust pH to 7.0.
- ยป O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 5 ยตL using a 25 ยตL Hamilton syringe.
- Final concentration: 5 mM.
- Preparation of 2.5 M stock solution (200 ยตL, dissolved in H2O) for use in 0.5-mL O2k-chamber:
- Prepare fresh everyday.
- Weigh 55 mg of pyruvic acid directly into a 0.5 mL Eppendorf tube.
- Add 175 ยตL H2O.
- Adjust pH to 7.0.
- ยป O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (0.5-mL O2k-chamber): 1 ยตL using a 10 ยตL Hamilton syringe.
- Final concentration: 5 mM.