Krumschnabel 1994 Experientia: Difference between revisions
No edit summary |
Gnaiger Caro (talk | contribs) No edit summary |
||
Line 14: | Line 14: | ||
|tissues=Liver | |tissues=Liver | ||
|preparations=Intact cells | |preparations=Intact cells | ||
|injuries= | |injuries=Ischemia-reperfusion | ||
|couplingstates=ROUTINE | |couplingstates=ROUTINE | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
}} | }} |
Revision as of 14:05, 16 June 2015
Krumschnabel G, Wieser W (1994) Inhibition of the sodium pump does not cause a stoichiometric decrease of ATP-production in energy limited fish hepatocytes. Experientia 50:483-485. |
Krumschnabel G, Wieser W (1994) Experientia
Abstract: In isolated goldfish hepatocytes underaerobic conditions the energy requirement for the sodium pump (calculated from Rb+ flux) is closely matched by the ouabain-sensitive fraction of oxygen consumption, whereas during in vitroanoxia (cyanide inhibition of the electron transport chain) the measured ATP demand of the sodium pump clearly exceeds ouabain-sensitive ATP production by anaerobic glycolysis. We conclude that when the energy status of cells is low, part or all of the ATP spared by the inhibition of a particular function may be used for fuelling other ATP-consuming functions. โข Keywords: goldfish, hepatocyte, sodium pump, protein synthesis, membrane-metabolic coupling, lactate, ouabain
โข O2k-Network Lab: AT Innsbruck OROBOROS
Labels: MiParea: Respiration
Stress:Ischemia-reperfusion
Tissue;cell: Liver Preparation: Intact cells
Coupling state: ROUTINE
HRR: Oxygraph-2k