Talk:O2k-Open Support

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Overview

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Frequently Asked Questions

» Oroboros FAQ

Technical service - pages in preparation

Technical service by telephone

We prefer to give technical assistance („trouble shooting“) via Email. We are aware that, especially if there is an urgent problem, some end-users would prefer to communicate by telephone. However, we are convinced that there are very good reasons for our approach:
Communication by Email
  • gives us the time to thoroughly read the customers problem description, analyze in detail the files sent to us and THINK about our proposals BEFORE suggesting them to you,
  • gives the customer time to read our suggestions carefully and to read any additional material (websites , manual !) that we proposed,
  • gives the customer time to properly collect (and document) the data that we asked her/him to send,
  • allows our technical service to be operated "by scientists, for scientists". The members of our technical service team work on their own project, keeping up to date with all technical development. However, this means they will not be available for phone conversation while running their experiments.
For a few special cases telephone conversations may actually make sense. In this case we will of course gladly arrange for such a phone call (planned beforehand).
When phoning Oroboros Instruments you will be connected to our administrative team. They will be glad to help you in administrative questions and point you to the right starting places for trouble shooting. Please understand that it will not be helpful to describe to them any technical details related to a problem on the phone. Even our technical staff prefer to get this information by e-mail, information relayed via non technical experts would be even less helpful. Therefore, this information should be sent by e-mail and include as much of the information gathered during trouble shooting (see the main page) as possible, including a DatLab file (not a screen shot) of an Oxygen sensor test.
Fasching Mario 09:35, 17 April 2013 (CEST)





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Different Temperature Settings Effects on initial O2 concentration in Chambers

I work with different temperature settings, e.g., 20, 30 37 celsius. Should I expect different initial O2 concentrations in chambers, to begin with?


Troubleshooting

  • The most frequent problems reported by customers

Most problems can be readily solved when consulting the O2k-Manual of the O2k. We thus ask our customers to see the relevant chapter of the Manual before contacting the customer support.


Sensor signal is not stable

1. Check if you ran through the SOPs for Calibration and quality control

Case examples:

Oxygen signal


One chamber works well, the other does not

Locating the problem:
1. If the problem persists, try to locate the problem running the following experiment, while permanently recording:
2. Fill the chambers with medium or water and air calibrate
3. Perform a stirrer test.
4. When the signal is stable, empty the chambers (keep recording!), mark the Event and describe it in the Event window
5. Remove the POS-sensors from the chambers, unscrew the OroboPOS from the Connectors and swap the POS-sensors between chambers
6. Insert the Connector-attached POS into chambers again, refill the chambers with pre-warmed medium, mark and denote the Event (e.g. Sensors swapped)
7. Let this run for a few minutes, then again empty the chambers, remove sensors from chambers, unscrew the OroboPOS from the Connectors and swap the POS-connectors between chambers (so you have the original combination of POS and Connector but at the other chamber)
8. Refill the chambers with pre-warmed medium, mark and denote the Event (e.g. Connectors swapped)
9. Let this run for a few minutes and then save the file (e.g. Swap components.DLD).


If you find that the problem follows the OroboPOS, this typically indicates that a POS-Service is required. In some case, a repeated POS-Service may be needed.

Should the problem be with the Connector, cleaning the OroboPOS-Connector is advised.


Sometimes cleaning of components does not solve the problem, but drying does:

Dry O2k components: In some cases, water (excessive spillage, high humidity) is the problem, causing problems at electrical connections.

1. Remove the OroboPOS with Connectors from the chamber
2. Unscrew the OroboPOS from the Connector
3. Put the Connector to 50°C for 2h for drying.
4. Gently dry the connecting part of the OroboPOS
5. (Application of a drop of contact oil (as previously recommended) is generally not required, but when done make sure only very little oil is applied to each of the golden pins and threads on POS connector and POS.)
6. Dry the socket where the Connector is plugged into the O2k, using a hair dryer or similar device.


Zero voltage is too high, zero oxygen cannot be reached

During Zero calibration (setting R0) the sensor signal (in Volt) at zero oxygen concentration is determined. If the signal does not decline rapidly to between 0% and 5% of the signal seen at air saturation then:
1. check your Dithionite powder. Dithionite may deteriorate over time. Thus, if even adding more does not help it must be renewed.
2. perform a POS-Service (sometimes a repeated service is required).

Case examples:

Oxygen signal
O2 calibration - DatLab


After performing the sensor service, mounting a new membrane and time for the sensor to stabilize, typical raw signals (in V) determined at air equilibration can be seen here: http://bioblast.at/index.php/Raw_signal


The glass chamber is dirty or appears opaque or the stirrer bar is jumping

Cleaning the glass chamber
Chamber assembly


Different signals between chambers

http://wiki.oroboros.at/index.php/MiPNet06.03_POS-calibration-SOP
http://wiki.oroboros.at/index.php/O2_calibration_-_DatLab
http://wiki.oroboros.at/index.php/Air_calibration
http://wiki.oroboros.at/index.php/Zero_calibration
http://wiki.oroboros.at/index.php/MiPNet19.18B_POS-service
http://wiki.oroboros.at/index.php/Instrumental_background_oxygen_flux