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Talk:MiPNet23.15 O2k-pH ISE-Module

From Bioblast

O2k Series B and C, pX upgrade (installed before 2011)

connecting the MS connector to the oxygraph
connecting the electrodes to the MS connector
Connect:To connect two electrodes (pH + reference electrode) to the MultiSensor BNC plug of the O2k (Series B and C), a MultiSensor Connector has to be installed. The MultiSensor Connector has a black cable with a male BNC plug and a 2 mm plug on its instrument side (facing the O2k), and a female BNC plug and a 2 mm plug on the opposite side for connecting the electrodes. Additionally, an Allen key and a cable with a 2 mm pin and a spade terminal are included in the MultiSensor Connector set.
First this additional cable should be attached to the O2k housing. Loosen one of the lower screws on the front side, bottom panel of the O2k (using the supplied Allen key), insert the connection of the thin black cable (spade terminal, red) and tighten the screw again. This additional cable provides a grounding connection to the O2k which improves signal stability. The cable can be left attached to the Oxygraph even when the MultiSensor Connector is not in use and not attached to the O2k.
For measurements connect the black cable of the MultiSensor Connector with the BNC plug to the multi-sensor BNC port of the Oxygraph, marked β€œpX”. Then plug the 2 mm pin of the cable attached to the Oxygraph housing into the 2 mm plug of the MultiSensor Connector that is situated on the same side as the black cable. To have the BNC cable and the 2 mm plug in correct positions versus the Oxygraph just turn the MultiSensor Connector over, if necessary. The reference electrode is then connected to the front side 2 mm plug and the measuring electrode (pH) to the front side BNC plug of the MultiSensor Connector.
setting the gain for Series A to C at the bottom side of the O2k
Gain: The gain and offset of both potentiometric MultiSensor channels can be adjusted by turning the O2k housing on its side. At the bottom of the O2k there are 4 adjustable screws and a label indicating their functions.
Turning the screws clockwise increases; counter clockwise decreases gain and offset settings. For pH measurements between pH 4 and 10 it should not be necessary to change these settings. However, the settings might have been changed before for work with a different electrode. If you have set up your system and get a raw voltage beyond +9 or -9 volts, decrease the gain by one or two counter clockwise turns of the screw, repeat if necessary. Importantly, the raw voltage displayed in DatLab is already the amplified signal. It is usually not necessary to change the offset setting.

O2k Series B and C, pX upgrade (installed after 2011-01-01)

Connect:For O2k Series B to C with a pX upgrade installed after 2010, pH and reference electrodes are directly connected to the plugs on the front side of the O2k housing, as described above for O2k Series D (and higher). No special MultiSensor Connector is needed for such instruments. Insert the connector of the pH electrode into the BNC plug (nearest to the chamber) and the connector of the reference electrode to the 2 mm pin plug beside the BNC plug (MiPNet22.11 O2k-FluoRespirometer manual).
Gain: The gain of both potentiometric MultiSensor channels can be adjusted by turning the O2k housing on its side. At the bottom of the O2k there are 2 rotating switches and a label indicating their functions. The gain can be set to 10, 20, 40, or 80. The factory setting is a gain of 20. Usually a gain of 20 will also be very suitable for pH measurements. For some specific, extremely high-resolution pH measurements a higher gain might be advantageous to avoid limitation of resolution by digital noise. It is expected that for most users there never will arise the necessity to change the gain setting.
If you have set up your system and get a raw voltage beyond +9 or -9 volts, decrease the gain. Importantly, the raw voltage displayed in DatLab is already the amplified signal.