Stirrer test

                  

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Stirrer test

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MitoPedia O2k and high-resolution respirometry: O2k-Open Support 

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Description

A stirrer test is performed in the Oroboros O2k for quick evaluation of the performance of the OroboPOS and for dynamic calibration. Stirring is stopped in both chambers and restarted after a selected period. The default period is 30 s, for experiments at 37 °C. At lower experimental temperature, this period should be prolonged (60 s at 25 °C). In the SOP (O2k Quality Control) for the O₂ sensor test, the stirrer test is performed in the 'open' chamber in conjunction with Air calibration. In general, the stirrer test can be performed equally with an open or closed chamber. Upon automatic re-start of the stirrer (On), the increase of the oxygen signal should be rapid and monoexponential.

DatLab 8

Last update: 2024-05-06

The stirrer test is initiated by pressing on Stirrer test or on the corresponding DL-Protocol window DL8-Protocol event in the sidebar.

DatLab 7

The stirrer test is initiated by pressing [F9] in DatLab or from the "Oroboros O2k" drop down menu.

Abbreviation: F9

Bioblast links: Oxygen signal - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>

Specific

» Oxygen signal

» O2k signal line

O2k-Procedures

» Air calibration

» Zero calibration

» High signal at zero oxygen

» POS calibration - static

» POS calibration - dynamic

» DL-Protocols

» Oxygen sensor test

» Stirrer test

» Open chamber

» Closed chamber

MiPNet O2k-Procedures

» POS-calibration

» O2k-Manual: POS-service

» O2k-cleaning

» O2k-Series G and DatLab 6: Calibration

General

» Oxygen, dioxygen, O₂

» Oxygen calibration - DatLab

» Oxygen solubility

» Oxygen solubility factor

» Oxygen pressure

» Concentration

» Activity

» Pressure - Pascal

» Barometric pressure

» High-resolution respirometry

» OroboPOS

» Polarographic oxygen sensor

» MitoFit Quality Control System

» Sensitivity

Other keyword lists

» Keywords: Concentration and pressure

MitoPedia O2k and high-resolution respirometry: DatLab