Startup O2k-Respirometer

From Bioblast
Jump to: navigation, search

Oroboros Instruments

News         O2k-Products         O2k-Purchase         O2k-Open Support         O2k-Publications         O2k-Workshops and Events         O2k-Network         O2k-Feedback         NextGen-O2k

Startup O2k-Respirometer


Description Startup O2k-Respirometer - the experimental system complete for basic high-resolution respirometry (HRR). The O2k-Respirometer includes the O2k-Main Unit with stainless steel housing, O2k-Assembly Kit, two OroboPOS (polarographic oxygen sensors) and OroboPOS-Service Kit, DatLab software, the ISS-Integrated Suction System, the O2k-Titration Set, and for performing high-resolution respirometry with reduced amounts of biological sample the O2k-sV-Module.

The O2k-Respirometer supports all add-on O2k-Modules of the Oroboros O2k, and is a component of the O2k-FluoRespirometer.

On-line display of oxygen flux (rate of respiration) is provided in addition to the conventional 'oxygraphic' plot of oxygen concentration over time. Highest signal stability minimizes the required amounts of biological sample, and provides the basis for resolution in the extreme low-oxygen range. Peltier temperature control provides a thermal stability at ±0.002 °C in the range of 4 °C to 47 °C at typical constant room temperature. Electronically controlled PVDF or PEEK stirrers are integrated in the two-chamber design of the O2k, and a barometric pressure transducer enables automatic oxygen calibrations implemented in the DatLab software.

The O2k is a sole source apparatus with no other instruments meeting its specifications.

Product ID 10022-03
Type O2k
Link Gnaiger_2008_POS
Startup O2k-Respirometer notitle.png

  • A PC is required (not included), connecting DatLab to the O2k via a USB port. For PC requirements, see here.


The Startup O2k-Respirometer consists of

TitleDescriptionProduct idProduct image
O2k-sV-ModuleO2k-sV-Module: Now included in all O2k-Packages, comprised of Duran glass chambers of 12 mm inner diameter specifically developed to perform high-resolution respirometry with reduced amounts of biological sample, and all components necessary for smaller operation volume (V) of 0.5 cm3 (0.5 mL). Current DatLab version is included in the delivery of this revolutionary module.

The O2k-sV-Module is compatible with all O2k-Series and it is designed specifically for respirometry using small amounts of sample.

The O2k-sV-Module cannot be used for simultaneous measurement of multiple parameters in the O2k-FluoRespirometer or in combination with the O2k-MultiSensor Modules.
OroboPOS-Service KitOroboPOS-Service_Kit, with O2k-Service Box including all oxygen sensor service accessories for membrane mounting and service of the OroboPOS.20610-03
ServiceBox G 2.jpg
DatLabDatLab is the O2k-Software for Data Acquisition & Analysis, specifically developed for high-resolution respirometry with the O2k-FluoRespirometer.

The newest DatLab version is DatLab 7.4, included in the O2k-FluoRespirometer.

The minimum computer requirements are Intel-Core-2 or equivalent CPU, 2GB RAM and Windows XP. However, we recommend Intel i5 or equivalent CPU, 4GB RAM, Windows 10 and SSD. For the proper display of DatLab on your computer, please make sure the “Language settings” are set to English.
ISS-Integrated Suction SystemISS-Integrated Suction System: Suction pump 230 or 120 V with stainless steel housing, 2 liter waste bottle, filter and tubing; for siphoning off excess medium from the O2k-Stopper and for emptying the O2k-Chambers. The ISS is included as a standard component of the O2k-FluoRespirometer. Media containing living cells or microorganisms, various poisons (inhibitors, uncouplers) and mixtures of proteins and substrates are safely disposed off in the 2-litre waste bottle.

The suction pump (230 V or 120 V) is enclosed in a stainless steel housing, which holds a readily accessible mains switch, two removable receptacles for the tip of the tubing, an easily removable and safe connection to the gas filter which further connects to the fully stabilized waste bottle. The waste bottle with filter can be simply removed for emptying, cleaning and drying.

For specific country configurations, see Product ID 28101, 28102, 28103.
O2k-Titration SetThe O2k-Titration Set consists of Hamilton microsyringes (6 x 10 mm3 and 3 spare plungers, 6 x 25 mm3, 1 x 50 mm3, 1 x 100 mm3, 1 x 500 mm3; fixed needles with rounded tips), provided in the Syringe Storage Box with Syringe Labels, a set of two Syringe Racks with Syringe Collars, and a set of two Tube Racks. The O2k-Titration Set is included in the O2k-FluoRespirometer and Startup O2k-Respirometer.20820-03
O2k-Titration Set.JPG
O2k-USB Flash DriveThe O2k-USB Flash Drive is a component of the Oroboros O2k containing: DatLab, O2k-Manual, O2k-Protocols, O2k-Publications, and info on O2k-Workshops.20900-02
O2k-Chamber HolderO2k-Chamber Holder (blue POM) for PVDF or PEEK stoppers, with O-ring\Viton\18x2 mm and V-ring\30-35-4.5 mm. Two units of this item are standard components mounted on the O2k-Main Unit.22100-01
Chamber holder PVDF Stopper.jpg
OroboPOS-ConnectorOroboPOS-Connector (blue POM), with male connection to OroboPOS sensor head (POS) and with cable and male plug fitting into O2k-Main Unit. Two units of this item are standard components of the O2k-Assembly Kit (O2k-FluoRespirometer), mounted on the OroboPOS-Holders at the O2k-Main Unit.22200-01
POS connector and cable connection.jpg
OroboPOS-HolderThe OroboPOS-Holder (POS-Holder), made from blue POM, is screwed into the copper block of the O2k-Main Unit, guiding the OroboPOS-Connector with the OroboPOS sensor head (POS) to the O2k-Chamber, and keeping the OroboPOS-Connector in a fixed position for sealing the O2k-Chamber with the OroboPOS-Seal Tip. In addition, the OroboPOS-Holder fixes the O2k-Chamber in an accurate rotational position by pressing against the angular cut of the glass chamber. Two units of this item are standard components mounted on the O2k-Main Unit.22300-01
Pos holder.JPG
O2k-ChamberO2k-Chamber: 16 mm inner diameter, Duran glass polished, with standard operation volume (V) of 2 cm3 (2 mL). Two units of this item are standard components of the O2k-FluoRespirometer. Separate glass chambers are available as spares. The optical properties of Duran allow application of fluorometric sensors (Duran optical properties).23100-01
Glass chamber.jpg
OroboPOSThe OroboPOS is a polarographic oxygen sensor (POS), with an amperometric mode of operation. The OroboPOS meets the highest quality criteria in terms of linearity, stability and sensitivity of the signal. The Clark type polarographic oxygen sensor (POS) remains the gold standard for measuring dissolved oxygen in biomedical, environmental and industrial applications over a wide dynamic oxygen range. It consists of a gold cathode, a silver/silverchloride anode and a KCl electrolyte reservoir separated from the sample by a 25 µm membrane (FEP). The main body of the OroboPOS is made of PEEK. With application of a polarization voltage (0.8 V), a current is obtained as an amperometric signal, which is converted to a voltage.26000-01

O2k-Core manual and selected protocols

» Contents: O2k Manual DatLab 7
ChapterViewSectionLast update
Gnaiger 2008 POSBioblast pdf
O2k-Protocols contents
Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley & Sons, Inc, Hoboken, NJ:327-52.
Gnaiger 2014 MitoPathwaysBioblast pdf
Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. Oroboros MiPNet Publications, Innsbruck:80 pp.
Gnaiger 2019 MitoPathwaysBioblast pdf 5th edition in preparation
Gnaiger E (2019) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Mitochondr Physiol Network 24.05. Oroboros MiPNet Publications, Innsbruck:112 pp.
MiPNet07.08 User informationBioblast pdf » Versions
O2k-Manual: user information. PLEASE STUDY THIS MANUAL.
MiPNet08.09 CellRespirationBioblast pdf  »Versions
O2k-Procedures contents
High-resolution respirometry and coupling control protocol with living cells: ROUTINE, LEAK, ET-pathway, ROX.
MiPNet12.01 Suppl T-issueBioblast pdf »Versions
O2k-Protocols contents
MitoPathways at the Q-junction: mouse skeletal muscle fibers.
MiPNet12.11 MitoRespirationBioblast pdf »Versions
Flux control ratios in isolated mitochondria. OXPHOS capacity and respiratory control.
MiPNet17.03 Shredder vs FibersBioblast pdf »Versions
Mitochondrial respiration in permeabilized fibers versus homogenate from fish liver and heart. An application study with the PBI-Shredder.
MiPNet19.18 O2k-Series G Core manualBioblast pdf »Versions
O2k-Core manual contents.
MiPNet19.18B POS-serviceBioblast pdf »Versions
Service of the polarographic oxygen sensor OroboPOS.
MiPNet19.18C DatLab 6: GuideBioblast pdf »Versions
DatLab-guide through the menus.
MiPNet19.18D O2k-Series G and DatLab 6: CalibrationBioblast pdf »Versions
O2k-calibration by DatLab 6.
MiPNet19.18E O2 flux analysis - DatLab 6Bioblast pdf »Versions
Oxygen flux analysis: DatLab real-time.
MiPNet21.16 DatLab 7 Innovations
About Oroboros
DatLab 7: innovations.
MiPNet22.16 DatLab 7 Innovations: DL-ProtocolsBioblast pdf » Versions
DatLab 7.4 Innovations: DL-Protocols
MiPNet23.12 DL-Protocols helpBioblast pdf » Versions
DatLab Protocols: Help DatLab 7.
MiPNet24.06 Oxygen flux analysis - DatLab 7.4Bioblast pdf » Versions
Oxygen flux analysis with DatLab 7.4.
MiPNet24.08 Safranin Analysis TemplateBioblast pdf » Versions
Excel template for safranin data analysis.
MiPNet24.09 General Template for Mt-membrane Potential AnalysisBioblast pdf » Versions
General template for mitochondiral membrane potential analysis.
Pesta 2012 Methods Mol BiolBioblast pdf
PMID: 22057559
Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibers from small biopsies of human muscle. Methods Mol Biol 810:25-58.

MitoPedia O2k and high-resolution respirometry: O2k hardware