Speichinger 2019 Innov Surg Sci
| Speichinger F, Kamphues C, Gratl A, Frese J, Greiner A, Kreis M (2019) High Resolution Respirometry (HRR) of the smooth muscle in the intestine. Innov Surg Sci. |
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Speichinger F, Kamphues C, Gratl A, Frese J, Greiner A, Kreis M (2019)
Event: DGCH Annual Congress 2019
High Resolution Respirometry has been shown as an effective tool to measure the mitochondrial respiration in striated muscle, as well as the measurement of citrate synthase activity (CSA) has been shown to give information of the amount of mitochondria in a tissue. In patients with peripheral arterial disease it has been shown that the function and the amount of mitochondria depends on the blood supply measured by the HRR. So far, there is no knowledge about the High Resolution Respiratory in the smooth muscle of the intestine.The investigation of the mitochondria in the smooth muscle of the intestine could be of great interest e.g. because the occurrence of anastomotic leakage is still a relevant complication in the colorectal surgery with poorly understood mechanism.
10 patients were included. The HRR and measurement of the CSA was performed with all samples. Some of them have been analysed with the TEM.
Through High Resolution Respirometry performed by using an Oxygraph-2k we were able to evaluate the mitochondrial respiration of the smooth muscle in the intestine. According to our findings of striated muscle in patients with peripheral arterial disease we observed similar results on a lower level, as expected due to the lower content of mitochondria in the smooth muscle. After correction for the CSA we could see similar ratios and proved that the HRR is transferable to smooth muscle tissue. Further evidence for the portability of smooth muscle we examine the Tunica muscularis of the intestine by transmission electron microscopy (TEM) and compared it with the results of straited muscle with similar results.
By High Resolution Respirometry using an Oxygraph-2k we were able to measure the activity and amount of mitochondria of the smooth muscle in the intestine. Thereby we established a new tool for further questions such as the function of mitochondria in reduced blood circulations in the intestine associated with anastomotic leakage.
• Bioblast editor: Plangger M
Labels: MiParea: Respiration Pathology: Cardiovascular
Organism: Human Tissue;cell: Other cell lines
HRR: Oxygraph-2k
Affiliations
- Charité - Unversitätsmedizin Berlin CBF, Berlin, Germany
